ELYS (AHCTF1) (NM_015446) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ELYS; MST108; MSTP108; TMBS62
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315386 representing NM_015446. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGAGCGGCGCTGTGGAAGTATGCGAGACTTAAGAGCTCAAGTGACTAGTGGTCTCCTGCCA TTTCCAGAAGTGACTCTTCAAGCCCTTGGAGAAGACGAAATAACATTAGAATCTGTGCTTCGTGGAAAG TTTGCTGCAGGGAAAAATGGACTTGCTTGCTTGGCTTGTGGTCCACAACTTGAGGTAGTAAACTCTATA ACAGGAGAGCGATTGTCTGCTTACAGATTCAGTGGAGTCAATGAACAGCCTCCTGTAGTTTTAGCTGTG AAAGAATTCTCTTGGCAGAAGAGAACTGGATTATTAATAGGATTGGAAGAAACAGAAGGGAGTGTTCTC TGTCTTTATGACCTTGGAATATCAAAAGTAGTTAAAGCAGTTGTTCTTCCTGGAAGGGTAACAGCTATT GAACCTATAATTAATCATGGAGGAGCCAGTGCAAGCACTCAGCATTTACATCCAAGTCTGCGATGGCTT TTTGGAGTGGCAGCTGTGGTCACTGATGTTGGACAGATCCTTCTTGTTGACCTATGTTTGGATGACTTG TCATGCAATCAAAATGAAGTTGAAGCATCAGATCTTGAAGTTCTAACTGGTATCCCAGCTGAAGTACCA CACATTAGAGAAAGTGTAATGAGACAAGGGCGCCATCTGTGTTTCCAGTTAGTAAGTCCAACAGGAACA GCTGTTTCAACTCTTAGTTACATAAGCAGGACAAATCAGCTTGCTGTAGGTTTTTCTGATGGCTATCTA GCACTTTGGAACATGAAAAGCATGAAAAGAGAATATTACATACAATTGGAAAGTGGACAAGTTCCTGTA TATGCTGTCACTTTTCAAGAACCTGAGAATGATCCTCGGAATTGCTGTTACTTGTGGGCTGTTCAGTCT ACACAAGATAGTGAAGGGGATGTTTTGAGTTTGCATCTGCTGCAGCTGGCCTTTGGTAATAGAAAGTGT TTGGCATCAGGACAAATCTTATATGAGGGGTTAGAATACTGTGAAGAAAGATACACCCTGGACCTGACA GGTGGCATGTTCCCTTTGAGGGGACAGACGAGTAATACCAAATTGTTGGGATGCCAGAGTATAGAGAAA TTTCGATCTCATGGTGACAGGGAGGAAGGCGTGAATGAAGCTCTATCGCCTGACACTAGTGTTTCAGTC TTTACCTGGCAGGTGAATATATATGGACAGGGAAAGCCTTCTGTATATTTGGGGCTTTTTGATATAAAT CGTTGGTATCATGCACAAATGCCAGATTCGTTAAGGTCAGGAGAATATCTACATAATTGCTCTTATTTT GCACTGTGGTCATTGGAGTCTGTTGTAAGTAGGACTTCTCCACATGGCATCTTGGATATATTAGTACAT GAGAGAAGTTTAAATAGAGGAGTCCCTCCTTCATATCCACCTCCCGAGCAGTTTTTTAATCCAAGCACT TATAATTTTGATGCCACTTGTTTGTTAAACTCGGGAGTTGTTCATTTAACTTGTACTGGCTTTCAGAAG GAGACTTTGACTTTTTTAAAGAAATCAGGTCCATCACTCAATGAACTCATTCCTGATGGTTATAATCGA TGTCTTGTAGCTGGCCTTCTTTCCCCAAGATTTGTTGATGTTCAGCCTTCCAGTTTAAGCCAAGAAGAA CAGTTAGAAGCTATATTGTCAGCAGCAATTCAGACTAGTTCCCTGGGACTTTTGACTGGTTATATCCGA AGATGGATAACAGAAGAACAACCAAATTCTGCCACTAATTTGCGCTTTGTTCTTGAATGGACGTGGAAT AAAGTGGTTCTCACAAAAGAGGAATTTGACAGACTATGTGTGCCATTATTTGATGGTTCGTGTCATTTC ATGGATCCACAAACTATACAGTCTATCCAGCAATGCTATTTGCTTCTTAGCAATCTTAATATAGTCTTG AGCTGTTTTGCATCAGAAGCCCGAGAGATCACTGAGAGAGGACTGATAGACTTAAGCAATAAGTTTGTG GTTTCCCACCTCATCTGTCAGTATGCACAAGTGGTTCTTTGGTTCTCTCATTCTGGGCTTTTACCAGAA GGCATAGATGATTCTGTGCAGTTGTCAAGGTTATGCTACAACTACCCTGTAATTCAGAACTACTACACC AGTCGTCGACAGAAGTTTGAGCGTTTATCAAGAGGGAAGTGGAATCCCGATTGCTTGATGATTGATGGA CTGGTTTCTCAGTTAGGAGAGCGAATTGAGAAGTTGTGGAAACGAGATGAAGGAGGCACAGGAAAATAT CCTCCTGCTAGTCTGCATGCAGTACTTGATATGTACCTATTAGACGGCGTTACTGAAGCAGCCAAACAC TCTATTACCATTTATTTGCTACTTGATATTATGTATTCCTTTCCCAACAAAACAGACACTCCCATTGAA TCTTTCCCAACTGTATTTGCCATTTCTTGGGGCCAAGTTAAACTTATTCAGGGGTTTTGGTTGATAGAT CATAATGACTATGAGAGTGGTTTGGATCTTTTGTTTCATCCAGCTACTGCAAAACCTTTGTCATGGCAA CATTCAAAGATTATTCAGGCATTCATGAGTCAGGGCGAGCACAGACAAGCCCTCAGATATATTCAGACA ATGAAGCCAACAGTGTCCAGTGGTAACGATGTTATCCTTCACCTCACTGTTTTGCTTTTTAATAGGTGT ATGGTTGAAGCCTGGAATTTTTTGCGGCAACATTGCAATAGGTTGAATATAGAGGAGTTACTGAAGCAC ATGTATGAAGTCTGTCAGGAAATGGGCTTGATGGAAGATTTACTGAAGTTACCATTTACAGACACTGAG CAGGAATGTTTAGTGAAATTTTTGCAGTCCAGTGCCAGCGTTCAGAATCATGAATTCCTTTTAGTGCAC CATTTGCAGCGTGCCAATTATGTGCCTGCCTTGAAGCTGAACCAAACTCTGAAGATTAATGTTATGAAT GATCGTGATCCTCGTTTGCGGGAGAGATCACTGGCTCGAAATTCTATATTAGACCAGTATGGAAAAATC CTTCCTAGAGTCCATCGAAAATTAGCCATTGAACGAGCTAAGCCTTATCATCTGTCAACATCATCAGTT TTTCGATTAGTTTCTAGACCCAAACCATTATCAGCAGTTCCAAAGCAAGTTGTAACAGGAACTGTGTTG ACAAGATCTGTTTTCATCAACAATGTGTTATCTAAAATTGGAGAAGTTTGGGCAAGCAAAGAACCTATA AATAGCACCACACCTTTCAATAGTTCTAAAATAGAAGAACCATCTCCTATAGTGTATTCGCTCCCAGCT CCAGAGCTGCCTGAGGCATTTTTTGGAACACCAATTTCAAAAGCATCACAAAAAATTTCTAGACTGCTA GATTTGGTTGTTCAGCCTGTCCCCCGGCCTTCTCAGTGTTCGGAGTTTATTCAGCAAAGCTCCATGAAA TCTCCTTTGTACCTAGTATCCCGTTCACTGCCCTCAAGTTCGCAATTAAAAGGATCGCCTCAGGCCATC TCCAGGGCTTCAGAATTACATTTGCTTGAAACTCCTCTTGTAGTTAAGAAAGCTAAAAGTTTGGCCATG TCAGTTACTACTTCTGGATTTTCTGAGTTCACTCCTCAGTCCATCCTGAGGTCTACTCTTCGATCAACA CCTTTAGCATCTCCCTCTCCATCACCTGGAAGGTCTCCTCAACGACTTAAAGAAACTAGAATTTCATTT GTGGAAGAAGATGTCCACCCAAAATGGATTCCTGGGGCTGCAGATGATAGCAAATTAGAAGTATTTACT ACACCTAAAAAATGTGCAGTTCCAGTGGAAACTGAATGGCTGAAGAGCAAAGATAGGACCACATCTTTT TTCCTGAACAGCCCTGAAAAGGAGCATCAAGAAATGGATGAGGGGTCACAAAGTTTAGAGAAACTGGAT GTGAGCAAAGGAAACAGCAGTGTTTCAATCACATCCGATGAGACTACCTTAGAGTATCAGGATGCACCG TCACCGGAAGACCTTGAAGAGACTGTTTTCACGGCCTCTAAGCCCAAAAGCTCTTCCACTGCACTAACT ACTAATGTAACTGAACAAACTGAAAAGGATGGAGATAAAGATGTATTTGCATCAGAAGTAACTCCTTCA GACCTACAGAAACAAATGGGCAATTTAGAAGATGCAGAAACAAAGGATCTCTTAGTTGCAGCAGAGGCA TTTTCAGAATTGAATCACTTAAGCCCGGTTCAAGGAACTGAAGCTTCTCTTTGTGCACCATCAGTCTAT GAAGGGAAAATCTTCACCCAGAAGTCCAAGGTACCAGTGTTGGACGAAGGATTAACATCTGTTGAAACC TACACCCCTGCAATTAGAGCAAATGACAATAAATCTATGGCTGATGTCCTTGGTGATGGTGGAAACTCC TCGCTCACTATCTCTGAAGGTCCTATTGTCTCTGAGCGCAGGCTTAACCAGGAAGTAGCGCTGAACTTA AAAGAAGATCATGAAGTAGAAGTTGGTGTACTAAAAGAAAGTGTTGACTTACCAGAAGAAAAGCTTCCA ATTTCTGACAGCCCTCCTGATACTCAAGAAATTCATGTGATTGAACAAGAAAAGCTTGAAGCTCAAGAT TCAGGAGAAGAGGCTAGGAATCTTTCATTTAATGAGTTATATCCCTCTGGAACACTTAAGCTTCAGTAC AATTTTGATACTATTGACCAACAGTTTTGTGACTTAGCTGATAACAAAGACACTGCTGAATGTGACATT GCTGAAGTAGATGGGGAACTTTTTGTGGCTCAAAGCAACTTTACCTTGATATTGGAAGGTGAAGAAGGA GAAGTTGAGCCAGGTGATTTTGCATCATCTGATGTGTTACCTAAAGCAGCTAACACAGCAACTGAAGAA AAACTTGTATGCAGTGGGGAAAATGATAATCATGGACAAATTGCAAATTTGCCATCTGCCGTAACTAGT GACCAAAAGTCCCAAAAAGTAGACACTTTACCATATGTGCCTGAACCTATTAAAGTAGCAATTGCAGAA AATTTACTAGATGTAATTAAAGACACAAGAAGTAAAGAAATTACTTCAGATACAATGGAACAGTCCATT CATGAAACAATACCTTTAGTGAGCCAAAACATAATGTGTCCCACTAAATTGGTCAAATCTGCATTTAAG ACTGCTCAGGAAACAAGCACAATGACTATGAATGTCAGCCAGGTTGATGACGTGGTTTCCTCCAAAACT CGTACGAGAGGTCAACGTATCCAAAACGTGAATGTCAAATCAGCACAACAGGAAGCATCAGCAGATGTT GCTACTCCTAAGATGCCAGGGCAGTCAGTCAGGAAGAAAACTAGGAAGGCAAAAGAAATTTCTGAAGCT TCTGAAAACATCTATTCTGATGTCAGAGGACTATCTCAGAACCAGCAAATACCTCAAAATTCTGTTACG CCTAGGAGAGGAAGGAGAAAGAAAGAAGTTAATCAGGACATACTAGAAAACACCAGTTCTGTGGAACAA GAATTACAGATCACTACAGGTAGGGAATCAAAAAGATTAAAATCATCTCAGCTGTTGGAACCAGCAGTT GAAGAAACTACTAAAAAAGAAGTTAAGGTTTCATCTGTTACAAAAAGGACTCCTAGAAGAATTAAAAGA TCTGTAGAAAATCAGGAAAGTGTTGAAATTATAAATGATCTAAAAGTTAGTACGGTAACAAGTCCTAGC AGAATGATCAGAAAATTGAGAAGTACTAATTTAGATGCTTCTGAAAATACAGGAAATAAGCAAGATGAT AAATCCAGTGACAAGCAGCTGCGTATTAAACATGTTAGAAGGGTCAGAGGGAGAGAAGTTAGTCCATCA GATGTGAGAGAAGACTCCAACCTTGAGTCATCTCAGTTGACTGTTCAAGCAGAATTTGATATGTCTGCC ATACCTAGAAAACGTGGTAGACCAAGAAAAATCAATCCATCTGAAGATGTAGGATCTAAGGCTGTTAAG GAAGAGAGAAGCCCCAAGAAGAAAGAAGCTCCCAGCATTAGAAGGAGATCTACAAGAAATACCCCAGCT AAAAGTGAAAATGTTGATGTTGGAAAACCAGCTTTAGGAAAATCCATTTTAGTGCCAAACGAGGAACTT TCGATGGTGATGAGCTCTAAGAAAAAACTTACAAAAAAGACTGAAAGTCAAAGCCAAAAACGTTCATTG CACTCAGTATCAGAAGAACGCACAGATGAAATGACACATAAAGAAACAAATGAGCAGGAAGAAAGATTG CTCGCCACAGCTTCCTTCACTAAATCATCCCGCAGCAGCAGGACTCGGTCTAGCAAGGCCATCTTGTTG CCGGACCTTTCTGAACCAAACAATGAGCCTTTATTTTCTCCAGCGTCAGAAGTTCCAAGGAAAGCAAAA GCTAAAAAAATAGAGGTTCCTGCACAGCTGAAAGAATTAGTTTCGGATTTATCTTCTCAGTTTGTCATC TCACCTCCTGCTTTAAGGAGCAGACAAAAAAACACATCCAATAAGAACAAGCTTGAAGATGAACTGAAA GATGATGCACAATCAGTAGAAACTCTGGGAAAGCCAAAAGCGAAACGAATCAGGACGTCAAAAACAAAA CAAGCAAGCAAAAACACAGAAAAAGAAAGTGCTTGGTCACCTCCTCCCATAGAAATTCGGCTGATTTCC CCCTTGGCTAGCCCAGCTGACGGAGTCAAGAGCAAACCAAGAAAAACTACAGAAGTGACAGGAACAGGT CTTGGAAGGAACAGAAAGAAACTGTCTTCCTATCCAAAGCAAATTTTACGCAGAAAAATGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015446
Insert Size	6828 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015446.4
RefSeq Size	8643 bp
RefSeq ORF	6828 bp
Locus ID	25909
UniProt ID	Q8WYP5
Domains	AT_hook
Protein Families	ES Cell Differentiation/IPS
MW	253.5 kDa
Gene Summary	Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.[UniProtKB/Swiss-Prot Function]

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