EST1A (SMG6) (NM_017575) Human Untagged Clone
CAT＃: SC315365
SMG6 (untagged)-Human Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG6), transcript variant 1
CNY 27360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C17orf31; EST1A; hSMG5/7a; SMG-6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315365 representing NM_017575.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAAGGGCTGGAGCGTGTGCGGATCTCCGCGTCGGAGCTGCGCGGGATCCTGGCTACTCTGGCC CCGCAGGCCGGGAGCAGAGAAAACATGAAGGAATTAAAGGAGGCCAGGCCGCGCAAAGATAACAGGCGT CCAGATCTGGAAATCTATAAGCCTGGCCTTTCTCGGCTAAGGAACAAGCCCAAAATCAAGGAACCCCCT GGGAGTGAGGAATTCAAAGATGAAATTGTTAATGACCGAGATTGCTCTGCTGTTGAAAATGGTACACAG CCCGTTAAAGATGTCTGCAAGGAACTGAACAACCAAGAGCAGAATGGTCCTATAGACCCAGAAAATAAT CGGGGACAAGAATCCTTTCCTAGGACTGCTGGACAAGAGGATCGTAGTCTAAAAATTATCAAAAGAACA AAGAAACCCGACCTGCAGATCTATCAGCCTGGACGACGTTTGCAGACTGTTAGCAAAGAATCCGCCAGT CGGGTGGAGGAGGAAGAAGTCCTCAACCAGGTAGAACAACTGAGAGTAGAGGAAGATGAGTGTAGGGGA AATGTTGCGAAGGAGGAAGTTGCGAATAAACCAGACAGGGCTGAGATAGAAAAGAGCCCAGGTGGTGGG AGAGTAGGGGCTGCAAAAGGAGAAAAAGGAAAGAGGATGGGAAAAGGGGAGGGGGTGAGGGAAACCCAC GACGACCCAGCCCGCGGGAGGCCGGGCTCCGCAAAGCGCTACTCCCGCTCAGACAAACGAAGGAATCGC TACCGCACGCGCAGCACCAGCTCAGCTGGCAGCAACAACAGCGCTGAGGGAGCTGGCCTGACGGATAAT GGATGTCGCCGCCGCCGACAGGATAGGACCAAGGAGAGGCCACGACTGAAGAAGCAAGTGTCTGTGTCC TCAACCGATTCCTTAGACGAGGACAGAATTGATGAGCCTGATGGATTAGGACCCAGGAGAAGTTCAGAA AGGAAGAGACATTTAGAAAGAAACTGGTCTGGCCGTGGGGAGGGTGAGCAGAAAAACAGTGCTAAAGAA TATCGAGGCACTCTTCGTGTCACTTTCGATGCAGAAGCCATGAACAAAGAGTCTCCCATGGTGAGGTCA GCCAGGGATGATATGGATAGAGGAAAGCCTGACAAAGGCTTGAGCAGTGGGGGCAAAGGCTCTGAGAAG CAGGAGTCCAAAAACCCGAAACAAGAACTTCGGGGTCGTGGTCGTGGCATTCTGATTTTGCCTGCCCAT ACCACCCTATCTGTCAATTCAGCAGGTTCTCCAGAGTCCGCGCCTTTGGGACCTCGGCTTTTGTTTGGA TCTGGTAGTAAGGGATCTCGGAGTTGGGGCCGTGGAGGCACCACACGCCGATTGTGGGACCCAAACAAT CCTGATCAGAAACCTGCTCTAAAGACTCAGACGCCCCAGCTACATTTCTTGGACACTGATGATGAAGTC AGCCCTACATCTTGGGGTGACTCACGCCAGGCTCAGGCATCTTACTATAAGTTTCAAAACTCTGACAAC CCCTATTATTACCCCCGGACACCAGGCCCTGCCTCCCAGTATCCCTATACGGGCTATAACCCTCTACAG TACCCAGTGGGCCCTACGAATGGTGTGTACCCAGGGCCTTACTACCCAGGCTACCCGACTCCGTCAGGA CAGTATGTGTGTAGCCCTCTACCTACCAGCACCATGAGTCCCGAGGAGGTAGAGCAGCACATGAGGAAC CTGCAGCAACAGGAGCTGCACAGGCTTCTCCGGGTGGCTGACAACCAGGAACTGCAGCTCAGCAACCTG CTCTCCAGGGACCGCATCAGTCCGGAGGGCCTGGAGAAGATGGCGCAACTCAGAGCTGAACTGCTGCAG CTATATGAGCGCTGTATTCTATTAGATATTGAGTTCTCTGATAATCAGAATGTGGATCAGATCCTGTGG AAGAATGCTTTCTATCAGGTGATTGAGAAGTTCAGGCAACTTGTCAAGGATCCGAATGTTGAGAACCCA GAACAGATTCGGAACAGACTTTTGGAGCTCTTGGATGAGGGTAGTGACTTCTTTGATAGTTTGCTTCAG AAGCTGCAGGTTACTTACAAGTTCAAACTGGAAGACTACATGGATGGTCTTGCCATTCGCAGCAAGCCA TTACGCAAGACAGTAAAATATGCCTTGATCAGTGCCCAGCGATGCATGATATGCCAAGGAGATATTGCT AGGTACCGGGAGCAAGCCAGTGATACAGCGAATTATGGGAAAGCACGCAGTTGGTACCTGAAGGCCCAG CACATTGCTCCCAAGAATGGGCGCCCCTATAACCAGTTGGCTTTGCTGGCAGTGTATACGAGGAGGAAG CTTGACGCTGTCTATTACTATATGCGCAGTTTAGCTGCCAGCAACCCTATCCTGACTGCCAAGGAGAGT CTCATGAGCTTGTTTGAAGAGACCAAGCGGAAGGCAGAACAGATGGAAAAGAAGCAACATGAGGAATTT GACCTGAGCCCTGACCAGTGGCGGAAAGGAAAGAAGTCTACTTTCCGGCATGTTGGAGATGACACCACT CGCCTGGAGATCTGGATTCATCCATCCCATCCACGGTCTTCCCAGGGCACTGAGTCTGGGAAGGATTCT GAGCAAGAGAATGGGCTGGGCAGCCTGAGTCCCAGTGATCTGAACAAAAGGTTCATCCTCAGTTTTCTC CATGCCCATGGGAAGCTGTTTACCCGGATTGGGATGGAGACATTCCCTGCAGTGGCTGAGAAGGTCCTC AAGGAGTTCCAGGTGTTACTGCAGCACAGCCCCTCTCCCATTGGAAGTACCCGCATGCTGCAGCTTATG ACCATCAATATGTTTGCAGTACACAACTCCCAGCTGAAAGACTGCTTCTCGGAGGAGTGCCGCTCTGTG ATCCAGGAACAAGCCGCAGCTCTGGGCTTGGCCATGTTTTCTCTACTGGTCCGCCGCTGCACCTGCTTA CTTAAGGAGTCCGCCAAAGCTCAGCTGTCCTCTCCTGAGGACCAGGATGACCAAGACGACATCAAGGTG TCTTCCTTTGTCCCGGACCTGAAGGAGCTGCTCCCCAGTGTCAAAGTCTGGTCAGATTGGATGCTCGGC TACCCGGACACCTGGAATCCTCCTCCCACATCCCTGGATCTGCCCTCGCATGTTGCTGTGGATGTATGG TCGACGCTGGCTGATTTCTGTAACATACTGACTGCAGTGAATCAGTCTGAGGTGCCACTGTACAAGGAC CCGGATGATGACCTCACCCTTCTTATCCTGGAAGAGGATCGGCTTCTCTCGGGCTTTGTCCCCTTGCTG GCTGCCCCTCAGGACCCCTGCTACGTGGAGAAAACCTCGGATAAGGTTATTGCAGCTGACTGCAAAAGG GTCACAGTGCTGAAGTATTTTCTGGAAGCCCTTTGTGGACAAGAAGAGCCTCTGCTGGCATTCAAGGGT GGAAAGTATGTGTCAGTGGCACCCGTCCCAGACACCATGGGAAAGGAAATGGGAAGCCAAGAGGGAACA CGACTGGAAGATGAGGAGGAGGATGTGGTGATTGAAGACTTTGAGGAAGATTCAGAGGCTGAAGGCAGC GGAGGCGAGGATGACATCAGGGAGCTTCGGGCCAAGAAGCTGGCTCTGGCCAGGAAGATAGCTGAGCAG CAGCGTCGCCAGGAAAAGATCCAGGCTGTCCTGGAGGACCACAGTCAGATGAGGCAGATGGAGCTCGAA ATCAGACCTTTGTTCCTCGTACCAGACACCAACGGCTTCATTGACCACCTGGCCAGTCTGGCGCGGCTG CTGGAGAGCAGGAAGTACATCCTGGTGGTGCCCCTCATCGTGATCAATGAGCTGGACGGCCTGGCCAAG GGGCAGGAGACAGACCACCGGGCTGGGGGCTACGCCCGTGTGGTACAAGAGAAGGCCCGCAAGTCCATC GAGTTCCTCGAGCAGCGATTCGAGAGTCGGGACTCTTGCCTGCGAGCCCTGACCAGCCGTGGCAATGAA CTCGAATCCATCGCCTTCCGCAGTGAGGACATCACTGGCCAGCTGGGTAACAACGATGATCTCATCCTG TCCTGCTGCCTCCACTACTGCAAAGACAAGGCTAAGGACTTCATGCCCGCCAGCAAAGAGGAGCCAATC CGGCTACTGCGGGAGGTGGTGCTGTTGACGGATGACCGGAACCTGCGTGTGAAGGCGCTCACAAGGAAT GTTCCTGTACGGGACATCCCAGCCTTCCTCACGTGGGCCCAGGTGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017575 |
| Insert Size | 4260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017575.4 |
| RefSeq Size | 5968 bp |
| RefSeq ORF | 4260 bp |
| Locus ID | 23293 |
| UniProt ID | Q86US8 |
| Domains | PINc |
| MW | 160.5 kDa |
| Gene Summary | This gene encodes a component of the telomerase ribonucleoprotein complex responsible for the replication and maintenance of chromosome ends. The encoded protein also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD. Alternatively spliced transcript variants encoding distinct protein isoforms have been described. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219132 | SMG6 (Myc-DDK-tagged)-Human Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG6), transcript variant 1 |
CNY 9712.00 |
|
| RC219132L3 | Lenti ORF clone of Human Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG6), transcript variant 1, Myc-DDK-tagged |
CNY 16060.00 |
|
| RC219132L4 | Lenti ORF clone of Human Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG6), transcript variant 1, mGFP tagged |
CNY 16060.00 |
|
| RG219132 | SMG6 (tGFP-tagged) - Human Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG6), transcript variant 1 |
CNY 15580.00 |
