ATP8B1 (NM_005603) Human Untagged Clone
CAT＃: SC315309
ATP8B1 (untagged)-Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1)
CNY 24040.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315309 representing NM_005603.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTACAGAAAGAGACTCAGAAACGACATTTGACGAGGATTCTCAGCCTAATGACGAAGTGGTTCCC TACAGTGATGATGAAACAGAAGATGAACTTGATGACCAGGGGTCTGCTGTTGAACCAGAACAAAACCGA GTCAACAGGGAAGCAGAGGAGAACCGGGAGCCATTCAGAAAAGAATGTACATGGCAAGTCAAAGCAAAC GATCGCAAGTACCACGAACAACCTCACTTTATGAACACAAAATTCTTGTGTATTAAGGAGAGTAAATAT GCGAATAATGCAATTAAAACATACAAGTACAACGCATTTACCTTTATACCAATGAATCTGTTTGAGCAG TTTAAGAGAGCAGCCAATTTATATTTCCTGGCTCTTCTTATCTTACAGGCAGTTCCTCAAATCTCTACC CTGGCTTGGTACACCACACTAGTGCCCCTGCTTGTGGTGCTGGGCGTCACTGCAATCAAAGACCTGGTG GACGATGTGGCTCGCCATAAAATGGATAAGGAAATCAACAATAGGACGTGTGAAGTCATTAAGGATGGC AGGTTCAAAGTTGCTAAGTGGAAAGAAATTCAAGTTGGAGACGTCATTCGTCTGAAAAAAAATGATTTT GTTCCAGCTGACATTCTCCTGCTGTCTAGCTCTGAGCCTAACAGCCTCTGCTATGTGGAAACAGCAGAA CTGGATGGAGAAACCAATTTAAAATTTAAGATGTCACTTGAAATCACAGACCAGTACCTCCAAAGAGAA GATACATTGGCTACATTTGATGGTTTTATTGAATGTGAAGAACCCAATAACAGACTAGATAAGTTTACA GGAACACTATTTTGGAGAAACACAAGTTTTCCTTTGGATGCTGATAAAATTTTGTTACGTGGCTGTGTA ATTAGGAACACCGATTTCTGCCACGGCTTAGTCATTTTTGCAGGTGCTGACACTAAAATAATGAAGAAT AGTGGGAAAACCAGATTTAAAAGAACTAAAATTGATTACTTGATGAACTACATGGTTTACACGATCTTT GTTGTTCTTATTCTGCTTTCTGCTGGTCTTGCCATCGGCCATGCTTATTGGGAAGCACAGGTGGGCAAT TCCTCTTGGTACCTCTATGATGGAGAAGACGATACACCCTCCTACCGTGGATTCCTCATTTTCTGGGGC TATATCATTGTTCTCAACACCATGGTACCCATCTCTCTCTATGTCAGCGTGGAAGTGATTCGTCTTGGA CAGAGTCACTTCATCAACTGGGACCTGCAAATGTACTATGCTGAGAAGGACACACCCGCAAAAGCTAGA ACCACCACACTCAATGAACAGCTCGGGCAGATCCATTATATCTTCTCTGATAAGACGGGGACACTCACA CAAAATATCATGACCTTTAAAAAGTGCTGTATCAACGGGCAGATATATGGGGACCATCGGGATGCCTCT CAACACAACCACAACAAAATAGAGCAAGTTGATTTTAGCTGGAATACATATGCTGATGGGAAGCTTGCA TTTTATGACCACTATCTTATTGAGCAAATCCAGTCAGGGAAAGAGCCAGAAGTACGACAGTTCTTCTTC TTGCTCGCAGTTTGCCACACAGTCATGGTGGATAGGACTGATGGTCAGCTCAACTACCAGGCAGCCTCT CCCGATGAAGGTGCCCTGGTAAACGCTGCCAGGAACTTTGGCTTTGCCTTCCTCGCCAGGACCCAGAAC ACCATCACCATCAGTGAACTGGGCACTGAAAGGACTTACAATGTTCTTGCCATTTTGGACTTCAACAGT GACCGGAAGCGAATGTCTATCATTGTAAGAACCCCAGAAGGCAATATCAAGCTTTACTGTAAAGGTGCT GACACTGTTATTTATGAACGGTTACATCGAATGAATCCTACTAAGCAAGAAACACAGGATGCCCTGGAT ATCTTTGCAAATGAAACTCTTAGAACCCTATGCCTTTGCTACAAGGAAATTGAAGAAAAAGAATTTACA GAATGGAATAAAAAGTTTATGGCTGCCAGTGTGGCCTCCACCAACCGGGACGAAGCTCTGGATAAAGTA TATGAGGAGATTGAAAAAGACTTAATTCTCCTGGGAGCTACAGCTATTGAAGACAAGCTACAGGATGGA GTTCCAGAAACCATTTCAAAACTTGCAAAAGCTGACATTAAGATCTGGGTGCTTACTGGAGACAAAAAG GAAACTGCTGAAAATATAGGATTTGCTTGTGAACTTCTGACTGAAGACACCACCATCTGCTATGGGGAG GATATTAATTCTCTTCTTCATGCAAGGATGGAAAACCAGAGGAATAGAGGTGGCGTCTACGCAAAGTTT GCACCTCCTGTGCAGGAATCTTTTTTTCCACCCGGTGGAAACCGTGCCTTAATCATCACTGGTTCTTGG TTGAATGAAATTCTTCTCGAGAAAAAGACCAAGAGAAATAAGATTCTGAAGCTGAAGTTCCCAAGAACA GAAGAAGAAAGACGGATGCGGACCCAAAGTAAAAGGAGGCTAGAAGCTAAGAAAGAGCAGCGGCAGAAA AACTTTGTGGACCTGGCCTGCGAGTGCAGCGCAGTCATCTGCTGCCGCGTCACCCCCAAGCAGAAGGCC ATGGTGGTGGACCTGGTGAAGAGGTACAAGAAAGCCATCACGCTGGCCATCGGAGATGGGGCCAATGAC GTGAACATGATCAAAACTGCCCACATTGGCGTTGGAATAAGTGGACAAGAAGGAATGCAAGCTGTCATG TCGAGTGACTATTCCTTTGCTCAGTTCCGATATCTGCAGAGGCTACTGCTGGTGCATGGCCGATGGTCT TACATAAGGATGTGCAAGTTCCTACGATACTTCTTTTACAAAAACTTTGCCTTTACTTTGGTTCATTTC TGGTACTCCTTCTTCAATGGCTACTCTGCGCAGACTGCATACGAGGATTGGTTCATCACCCTCTACAAC GTGCTGTACACCAGCCTGCCCGTGCTCCTCATGGGGCTGCTCGACCAGGATGTGAGTGACAAACTGAGC CTCCGATTCCCTGGGTTATACATAGTGGGACAAAGAGACTTACTATTCAACTATAAGAGATTCTTTGTA AGCTTGTTGCATGGGGTCCTAACATCGATGATCCTCTTCTTCATACCTCTTGGAGCTTATCTGCAAACC GTAGGGCAGGATGGAGAGGCACCTTCCGACTACCAGTCTTTTGCCGTCACCATTGCCTCTGCTCTTGTA ATAACAGTCAATTTCCAGATTGGCTTGGATACTTCTTATTGGACTTTTGTGAATGCTTTTTCAATTTTT GGAAGCATTGCACTTTATTTTGGCATCATGTTTGACTTTCATAGTGCTGGAATACATGTTCTCTTTCCA TCTGCATTTCAATTTACAGGCACAGCTTCAAACGCTCTGAGACAGCCATACATTTGGTTAACTATCATC CTGACTGTTGCTGTGTGCTTACTACCCGTCGTTGCCATTCGATTCCTGTCAATGACCATCTGGCCATCA GAAAGTGATAAGATCCAGAAGCATCGCAAGCGGTTGAAGGCGGAGGAGCAGTGGCAGCGACGGCAGCAG GTGTTCCGCCGGGGCGTGTCAACGCGGCGCTCGGCCTACGCCTTCTCGCACCAGCGGGGCTACGCGGAC CTCATCTCCTCCGGGCGCAGCATCCGCAAGAAGCGCTCGCCGCTTGATGCCATCGTGGCGGATGGCACC GCGGAGTACAGGCGCACCGGGGACAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_005603 |
| Insert Size | 3756 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005603.4 |
| RefSeq Size | 5949 bp |
| RefSeq ORF | 3756 bp |
| Locus ID | 5205 |
| UniProt ID | O43520 |
| Domains | Hydrolase |
| Protein Families | Transmembrane |
| MW | 143.7 kDa |
| Gene Summary | This gene encodes a member of the P-type cation transport ATPase family, which belongs to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. Mutations in this gene may result in progressive familial intrahepatic cholestasis type 1 and in benign recurrent intrahepatic cholestasis. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224841 | ATP8B1 (Myc-DDK-tagged)-Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1) |
CNY 13064.00 |
|
| RC224841L1 | Lenti ORF clone of Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1), Myc-DDK-tagged |
CNY 14350.00 |
|
| RC224841L2 | Lenti ORF clone of Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1), mGFP tagged |
CNY 15464.00 |
|
| RC224841L3 | Lenti ORF clone of Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1), Myc-DDK-tagged |
CNY 14350.00 |
|
| RC224841L4 | Lenti ORF clone of Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1), mGFP tagged |
CNY 14350.00 |
|
| RG224841 | ATP8B1 (tGFP-tagged) - Human ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1) |
CNY 14664.00 |
