CEP135 (NM_025009) Human Untagged Clone
CAT＃: SC315219
CEP135 (untagged)-Human centrosomal protein 135kDa (CEP135)
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CEP4; KIAA0635; MCPH8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315219 representing NM_025009.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCTGGGATACCGCCAG ACTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTAGTTCATACAACTGAGAGC CTTCGGCAATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAAAGTGCCAATTTTGATTTTGTTTTGGAA CCCTATAAACTTGAAAATGCAAGATTGAGTAGAGAAAATAATGAATTATACCTAGAGTTAATGAAACTG AGAGAACATTCAGACCAACACGTTAAAGAGTTGAAAACTTCATTGAAGAAATGTGCACGTGAAACAGCT GATCTGAAATTTCTGAATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAG AATGAAAGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAGAAA AGAAGTATTGCTTTCAGGCGCCAGCGTATGCAAATTGATGAACCGGTTCCTCCCTCTGAAGTCAGTTCA TATCCAGTTCCTCAACCAGATGACCCTTACATTGCAGACCTCCTTCAAGTGGCTGATAACAGGATTCAA GAACTTCAACAGGAAGTCCACCAGCTACAAGAAAAGTTAGCAATGATGGAAAGTGGGGTGAGAGACTAT AGCAAGCAGATTGAGCTAAGAGAACGAGAGATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCC CCTGATGTCCTTTCTCTGGAGTCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAG GTTGACTTTCTTCAGCAAGCTAATAAAGACCTGGAGAAGCGTATACGAGAGCTTATGGAAACCAAGGAA ACAGTGACATCTGAAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATTAACTGAAATA GATCAGTTAGCACAGCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCTGATAAAGAGCTTGGG GAAGCAAAGAAAGAGATTAAAAGAAAGCTCTCTGAAATGCAGGATCTTGAAGAAACAATGGCAAAACTT CAGCTGGAATTGAACTTATGCCAGAAAGAAAAGGAGAGACTGAGTGATGAACTCCTTGTAAAATCAGAC CTAGAAACTGTTGTTCATCAGCTTGAACAAGAAAAGCAAAGACTTAGCAAAAAAGTTGAAAGTTTTGCA GTTACAGAACGACAACTTACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGAC AGGTCACCTTCTCGTTTAGATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAG CTAGAGAGACTCCAACATATAATACAGCGAAGATCTTGCTCTACAAGTTATAGCGCACGTGAAAAAAGT TCAATATTTAGAACACCAGAAAAGGGTGATTACAATTCAGAAATTCATCAGATCACAAGAGAAAGAGAT GAACTTCAGCGTATGCTAGAAAGATTTGAAAAATATATGGAAGATATACAGTCCAATGTTAAATTATTG ACAGCAGAAAGAGATAAACTAAGTGTCTTATATAATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAG GAATCCACCCAAACCACAGCACCCCATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTA TCTGACTTAAGAAGAATTATGGCAGAAAAGGAAGCTTTAAGAGAAAAATTAGAGCATATTGAAGAAGTG AGTCTTTTTGGAAAATCAGAATTAGAGAAAACTATTGAACATTTGACATGTGTTAATCATCAGCTTGAA AGCGAAAAATATGAATTAAAGTCTAAAGTGTTAATAATGAAAGAAACAATAGAGTCGTTAGAGAACAAA TTAAAAGTCCAAGCTCAAAAATTTAGCCATGTGGCTGGTGACTCATCTCATCAGAAAACAGAGGTGAAC TCACTTAGGATAGTGAATGAGCAGCTACAGCGGTCAGTTGATGACTATCAGCACCGACTTTCCATAAAA AGAGGTGAACTTGAATCAGCCCAAGCACAAATTAAAATACTGGAGGAAAAGATAGATGAACTAAACCTT AAGATGACTTCACAGGATGAGGAGGCTCATGTAATGAAAAAGACCATTGGTGTTATTGATAAAGAAAAA GACTTTCTCCAGGAGACTGTAGATGAGAAGACAGAAAAGATTGCAAATTTGCAAGAAAACCTAGCTAAT AAAGAAAAAGCTGTTGCTCAAATGAAGATAATGATCTCAGAGTGTGAATCATCTGTGAACCAGCTGAAA GAAACATTGGTTAATCGAGATCGTGAGATAAACAGCCTCCGGCGCCAGCTTGATGCAGCTCACAAAGAA CTCGATGAAGTAGGAAGATCTAGAGAAATCGCTTTTAAGGAAAACAGAAGACTGCAAGATGACCTGGCT ACAATGGCAAGAGAAAATCAAGAAATCTCATTGGAATTGGAAGCAGCAGTGCAAGAAAAAGAAGAAATG AAGAGCAGAGTTCATAAATACATAACAGAGGTGTCACGATGGGAGAGCTTAATGGCTGCCAAGGAAAAA GAAAATCAAGATTTGTTAGATAGATTTCAGATGCTTCATAACCGTGCTGAAGACTGGGAGGTCAAAGCC CATCAAGCTGAGGGAGAAAGCAGCTCAGTTCGACTGGAACTTCTTTCTATTGACACTGAGAGGAGACAT CTTCGAGAAAGAGTGGAGCTATTAGAAAAAGAAATTCAAGAGCACATAAATGCCCATCATGCTTATGAA TCTCAGATCTCATCAATGGCAAAAGCCATGTCTCGATTAGAAGAAGAGCTGAGACATCAAGAAGATGAG AAAGCAACAGTATTAAATGACTTGTCATCTCTTAGAGAACTTTGCATTAAACTTGATTCAGGCAAAGAT ATTATGACCCAGCAATTGAATTCGAAAAACCTTGAGTTTGAGAGGGTTGTGGTGGAATTAGAAAATGTA AAGTCAGAGTCAGACCTACTGAAAAAACAACTTTCAAATGAGAGACATACAGTTAAAAACCTCGAATCA TTGTTGGCTACAAACAGAGATAAAGAATTTCATTCTCACTTAACCTCCCACGAGAAGGATACAGAAATC CAGCTACTTAAGGAGAAGTTAACCCTTTCTGAAAGCAAATTAACTAGTCAAAGCCGGGAAAACACCATG CTTCGAGCTAAAGTGGCACAGTTACAAACAGATTATGATGCTCTGAAAAGGCAGATCTCAACTGAAAGA TACGAACGAGAACGAGCAATCCAAGAGATGCGTCGACATGGTCTTGCTACACCACCCCTTAGTTCCACT CTGAGGTCTCCTTCACATTCTCCTGAACATAGAAATGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025009 |
| Insert Size | 3423 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025009.4 |
| RefSeq Size | 5760 bp |
| RefSeq ORF | 3423 bp |
| Locus ID | 9662 |
| UniProt ID | Q66GS9 |
| MW | 133.5 kDa |
| Gene Summary | This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224008 | CEP135 (Myc-DDK-tagged)-Human centrosomal protein 135kDa (CEP135) |
CNY 7936.00 |
|
| RC224008L3 | Lenti-ORF clone of CEP135 (Myc-DDK-tagged)-Human centrosomal protein 135kDa (CEP135) |
CNY 13300.00 |
|
| RC224008L4 | Lenti-ORF clone of CEP135 (mGFP-tagged)-Human centrosomal protein 135kDa (CEP135) |
CNY 13300.00 |
|
| RG224008 | CEP135 (tGFP-tagged) - Human centrosomal protein 135kDa (CEP135) |
CNY 12540.00 |
