SLC12A1 (NM_000338) Human Untagged Clone
CAT＃: SC315178
SLC12A1 (untagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1
CNY 8424.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BSC1; NKCC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_000338 |
| Insert Size | 3300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000338.2 |
| RefSeq Size | 4717 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 6557 |
| UniProt ID | Q13621 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 121.5 kDa |
| Gene Summary | This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010] Transcript Variant: This variant (1) encodes isoform A. Isoforms A and F are the same length but differ in the region of amino acids 214-238. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216145 | SLC12A1 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1 |
CNY 7656.00 |
|
| RC216145L1 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1, Myc-DDK-tagged |
CNY 10056.00 |
|
| RC216145L2 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1, mGFP tagged |
CNY 10260.00 |
|
| RC216145L3 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1, Myc-DDK-tagged |
CNY 10260.00 |
|
| RC216145L4 | Lenti ORF clone of Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1, mGFP tagged |
CNY 10260.00 |
|
| RG216145 | SLC12A1 (tGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1 |
CNY 9220.00 |
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