FBH1 (NM_032807) Human Untagged Clone
CAT＃: SC315174
FBXO18 (untagged)-Human F-box protein, helicase, 18 (FBXO18), transcript variant 1
CNY 7624.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Fbx18; FBXO18; hFBH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315174 representing NM_032807.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCTACGAGGTGACTTCAGGCTGCCATTGGACCTGTCAAGTGCCTGAGTCATGTGATAATGGGCTA CATTGCGCAGGGCCCCTGGGCCATCTCCACAGGAGATGCCAGAGGACGAGTGCCCACTTGCTGGTCTTC ACAGAGCACGCTGAAATGAGACGGTTTAAGCGGAAGCATCTTACTGCCATTGACTGCCAGCATTTGGCT CGGAGTCACTTGGCTGTGACCCAGCCCTTCGGTCAAAGATGGACAAACAGAGATCCGAACCATGGTCTC TATCCTAAACCGAGAACAAAAAGAGGGAGTAGGGGTCAGGGAAGTCAAAGATGCATCCCTGAGTTCTTC CTAGCAGGCAAGCAGCCGTGCACCAATGACATGGCCAAAAGCAATTCTGTTGGCCAGGACAGCTGTCAG GACTCTGAGGGTGACATGATCTTTCCTGCAGAGAGCAGCTGTGCACTGCCTCAGGAAGGCAGTGCAGGG CCGGGCTCACCAGGGTCTGCCCCGCCCTCCAGGAAGCGGTCTTGGTCCTCTGAGGAAGAGAGTAACCAG GCTACCGGGACCAGCCGGTGGGATGGAGTTTCTAAGAAAGCTCCACGGCACCATTTGTCTGTGCCATGC ACAAGGCCTAGGGAGGCCAGGCAAGAAGCAGAGGACAGTACGTCTCGGCTCTCTGCGGAGTCTGGTGAA ACCGACCAAGATGCTGGGGACGTGGGTCCTGATCCCATTCCTGACTCATACTATGGGCTTCTTGGGACC TTGCCCTGCCAGGAAGCACTGAGCCACATTTGCAGCCTGCCTAGTGAGGTCCTGAGGCACGTGTTTGCC TTCCTCCCGGTGGAAGACCTCTATTGGAACCTGAGCTTGGTGTGCCACTTGTGGAGGGAGATCATCAGT GACCCGCTGTTCATTCCTTGGAAGAAGCTGTACCATCGATACCTGATGAATGAAGAGCAAGCTGTCAGC AAAGTGGACGGCATCCTGTCTAACTGTGGCATAGAAAAGGAGTCAGACCTGTGTGTGCTGAACCTCATA CGATACACAGCCACCACTAAGTGCTCTCCGAGTGTGGATCCCGAGAGGGTGCTGTGGAGTCTGAGGGAC CACCCCCTCCTCCCCGAGGCTGAGGCGTGTGTGCGGCAACACCTCCCCGACCTCTACGCTGCTGCCGGG GGTGTCAACATCTGGGCCCTGGTGGCGGCTGTGGTGCTCCTCTCCAGCAGTGTGAATGACATCCAGCGA CTGCTCTTCTGCCTCCGGAGACCCAGCTCCACGGTGACCATGCCAGATGTCACCGAGACCCTGTACTGC ATAGCCGTGCTTCTCTACGCCATGAGGGAGAAGGGGATTAACATCAGCAATAGGATTCACTACAACATT TTCTATTGCCTATATCTTCAGGAGAATTCCTGCACTCAGGCCACAAAAGTTAAAGAGGAGCCATCTGTC TGGCCAGGCAAGAAAACCATCCAACTTACACATGAACAACAGCTGATTCTGAATCACAAGATGGAACCT CTCCAGGTGGTGAAAATTATGGCCTTTGCCGGCACTGGGAAGACCTCAACGCTGGTCAAGTATGCAGAG AAGTGGTCTCAGAGCAGGTTTCTGTATGTGACATTCAACAAGAGCATCGCAAAGCAGGCCGAACGCGTC TTCCCCAGCAACGTCATCTGCAAAACCTTCCACTCCATGGCCTACGGGCACATAGGGCGGAAGTACCAG TCAAAGAAGAAGTTGAATCTCTTCAAGTTAACACCCTTCATGGTCAACTCCGTCCTTGCTGAAGGGAAG GGTGGATTCATAAGAGCCAAGCTTGTGTGTAAGACTCTAGAAAACTTCTTTGCCTCGGCTGACGAAGAG CTGACCATTGATCACGTGCCTATTTGGTGTAAGAACAGCCAAGGACAGAGAGTCATGGTTGAGCAGAGT GAAAAACTGAATGGTGTCCTTGAAGCGAGCCGCCTCTGGGATAACATGCGGAAGCTGGGGGAGTGCACA GAAGAGGCGCACCAGATGACTCATGACGGCTACTTGAAACTCTGGCAGCTGAGCAAGCCTTCGCTGGCC TCTTTTGACGCCATCTTTGTGGATGAGGCCCAGGACTGCACACCAGCTATCATGAACATAGTTCTGTCT CAGCCATGTGGGAAAATCTTTGTAGGGGACCCGCACCAGCAGATCTATACCTTCCGGGGTGCGGTCAAC GCCCTGTTCACAGTGCCCCACACCCACGTCTTCTATCTCACGCAGAGTTTTCGGTTTGGTGTGGAAATA GCTTATGTGGGAGCTACTATCTTGGATGTTTGCAAGAGAGTCAGGAAAAAGACTTTGGTTGGAGGAAAC CATCAGAGTGGCATTAGAGGTGACGCAAAGGGGCAAGTGGCCTTGTTGTCCCGGACCAACGCCAACGTG TTTGATGAGGCCGTACGGGTGACGGAAGGGGAATTCCCTTCAAGGATACATTTGATTGGGGGGATTAAA TCATTTGGATTGGACAGAATCATTGATATTTGGATCCTTCTTCAGCCAGAGGAAGAACGGAGGAAACAA AACCTCGTCATTAAAGACAAATTTATCAGAAGATGGGTGCACAAAGAAGGCTTTAGTGGCTTCAAGAGG TATGTGACCGCTGCCGAGGACAAGGAGCTTGAAGCCAAGATCGCAGTTGTTGAAAAGTATAACATCAGG ATTCCAGAGCTGGTGCAAAGGATAGAAAAATGCCATATAGAAGATTTGGACTTTGCAGAGTACATTCTG GGCACTGTGCACAAAGCCAAAGGCCTGGAGTTTGACACTGTGCATGTTTTGGATGATTTTGTGAAAGTG CCTTGTGCCCGGCATAACCTGCCCCAGCTTCCGCACTTCAGAGTTGAGTCATTTTCTGAGGATGAATGG AATTTACTGTATGTTGCAGTAACTCGAGCCAAGAAGCGTCTCATCATGACCAAATCATTGGAAAACATT TTGACTTTGGCTGGGGAGTACTTCTTGCAAGCAGAGCTGACAAGCAACGTCTTAAAAACAGGCGTGGTG CGCTGCTGCGTGGGACAGTGCAACAATGCCATCCCTGTTGACACCGTCCTTACCATGAAGAAGCTGCCC ATCACCTATAGCAACAGGAAGGAAAACAAGGGGGGCTACCTCTGCCACTCCTGTGCGGAGCAGCGCATC GGGCCCCTGGCGTTCCTGACAGCCTCCCCGGAGCAGGTGCGCGCCATGGAGCGCACTGTGGAGAACATC GTACTGCCCCGGCATGAGGCCCTGCTCTTCCTCGTCTTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_032807 |
| Insert Size | 3285 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032807.3 |
| RefSeq Size | 3718 bp |
| RefSeq ORF | 3285 bp |
| Locus ID | 84893 |
| UniProt ID | Q8NFZ0 |
| Domains | F-box |
| Protein Families | Druggable Genome |
| MW | 123.4 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214006 | FBXO18 (Myc-DDK-tagged)-Human F-box protein, helicase, 18 (FBXO18), transcript variant 1 |
CNY 7616.00 |
|
| RC214006L3 | Lenti ORF clone of Human F-box protein, helicase, 18 (FBXO18), transcript variant 1, Myc-DDK-tagged |
CNY 12830.00 |
|
| RC214006L4 | Lenti ORF clone of Human F-box protein, helicase, 18 (FBXO18), transcript variant 1, mGFP tagged |
CNY 10016.00 |
|
| RG214006 | FBXO18 (tGFP-tagged) - Human F-box protein, helicase, 18 (FBXO18), transcript variant 1 |
CNY 11970.00 |
