MRTFA (NM_020831) Human Untagged Clone
CAT＃: SC314943
MKL1 (untagged)-Human megakaryoblastic leukemia (translocation) 1 (MKL1)
CNY 18050.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BSAC; MAL; MKL; MKL1; MRTF-A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314943 representing NM_020831.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAG GACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAA GAGACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGAC CTCAATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAG TCCAGCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTC GATGAGGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCG TCACCCCTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTTGTG TCTCAACTTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCTCCTCTGCCTCCC CCACCTCTGCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACTGCCAAGTCCACCCCCACACTC ATTAAGCAAAGCCAACCCAAGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAG CCAAAGGTGAAGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCC CCCATGGACTCATCCTACGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAAC CAGCAGCAGCAGCAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCC CTGGGAAGCAGCGGGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCC CCTGGGCCCTGTGGGCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCC AACCTGGACGACATGAAGGTGGCAGAGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGC ACCAAAACTGAGCTGATTGAGCGCCTTCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCC AAGGCCCCTGCCGCCACCTCTATCCTGCACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGG CTGAGCACGGGGCCAGCCCTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCC AGCAGTGGGGTGGTGAAGTTTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGC TCACTGCTCAGCACGGGCGATGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCT CTGACGCAGCTGACCCTGCAGGCCTCGCCACTGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGG TCCTGTTGCCTGAGCCCTGGGGGGCGGGCGGAGCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAG AAAGACAAGCAGATCGAGGCGCTGACGCGCATGCTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAG CTGCAGCTGGAGCAGGAGAAGCGAGCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTG AAGCAGGAGAACAGCTTCTCCAGCTGCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAAC CCCAGCCTGGCGGCCCCAGCCACCAACCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTG GTGGTGAAGCAGGAAGCCTTGCAGCCTGAGCCCGAGCCGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCT CAGGGCCCCAGCCTCATCAAGGGGGTTGCACCTCCCACCCTCATCACCGACTCCACAGGGACCCACCTT GTCCTCACCGTGACCAATAAGAATGCAGACAGCCCTGGCCTGTCCAGTGGGAGCCCCCAGCAGCCCTCG TCCCAGCCTGGCTCTCCAGCGCCTGCCCCCTCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTC TTTGGGACCCCCACTTCTCTGCTGAAGAAGGAACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCC AAACAGCAGGAAAATGGTTCCTCAAGCCAGCAGATGGACGACCTGTTTGACATTCTCATTCAGAGCGGA GAAATTTCAGCAGATTTCAAGGAGCCGCCATCCCTGCCAGGGAAGGAGAAGCCATCCCCGAAGACAGTC TGTGGGTCCCCCCTGGCAGCACAGCCATCACCTTCTGCTGAGCTCCCCCAGGCTGCCCCACCTCCTCCA GGCTCACCCTCCCTCCCTGGACGCCTGGAGGACTTCCTGGAGAGCAGCACGGGGCTGCCCCTGCTGACC AGTGGGCATGACGGGCCAGAGCCCCTTTCCCTCATTGACGACCTCCATAGCCAGATGCTGAGCAGCACT GCCATCCTGGACCACCCCCCGTCACCCATGGACACCTCGGAATTGCACTTTGTTCCTGAGCCCAGCAGC ACCATGGGCCTGGACCTGGCTGATGGCCACCTGGACAGCATGGACTGGCTGGAGCTGTCGTCAGGTGGT CCCGTGCTGAGCCTAGCCCCCCTCAGCACCACAGCCCCCAGCCTCTTCTCCACAGACTTCCTCGATGGC CATGATTTGCAGCTGCACTGGGATTCCTGCTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020831 |
| Insert Size | 2796 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020831.4 |
| RefSeq Size | 4550 bp |
| RefSeq ORF | 2796 bp |
| Locus ID | 57591 |
| UniProt ID | Q969V6 |
| Domains | SAP, RPEL |
| Protein Families | Transcription Factors |
| MW | 98.9 kDa |
| Gene Summary | The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (1) has a shorter N-terminus, compared to isoform 1. Both variants 1 and 2 encode the same isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221212 | MKL1 (Myc-DDK-tagged)-Human megakaryoblastic leukemia (translocation) 1 (MKL1) |
CNY 10224.00 |
|
| RC221212L3 | Lenti ORF clone of Human megakaryoblastic leukemia (translocation) 1 (MKL1), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC221212L4 | Lenti ORF clone of Human megakaryoblastic leukemia (translocation) 1 (MKL1), mGFP tagged |
CNY 9030.00 |
|
| RG221212 | MKL1 (tGFP-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1) |
CNY 11824.00 |
