SPAG1 (NM_003114) Human Untagged Clone
CAT＃: SC314935
SPAG1 (untagged)-Human sperm associated antigen 1 (SPAG1), transcript variant 1
CNY 17860.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8; SP75; TPIS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314935 representing NM_003114.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCACCAAAGATTATCCATCATTGTGGGGCTTTGGAACAACAAAAACATTCAAAATTCCCATTGAA CATCTAGATTTCAAATACATTGAAAAATGTTCAGATGTTAAACATCTGGAAAAAATTCTTTGCGTGCTC AGATCTGGTGAGGAAGGATATTATCCTGAACTTACAGAATTTTGTGAAAAGCATCTTCAAGCCTTGGCC CCTGAAAGCAGAGCTTTGAGGAAAGATAAACCAGCAGCAACAGCAGCCAGTTTTACAGCTGAAGAATGG GAAAAAATTGATGGTGATATAAAGAGTTGGGTATCAGAAATTAAAAAAGAAGAAGATAAAATGCACTTT CATGAAACTGAGACATTTCCAGCAATGAAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCTGTCTT CATGTAGGCAAGGAAAAATATTCTAAAAGACCAACTAAAAAGAAAACTCCAAGGGATTACGCGGAATGG GATAAATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATTACAAAGAAAAGACGGTAATAGAC AAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAGGTCTAACTGAGAAAGAAAAGGATTTT CTTGCCACTCGTGAAAAGGAGAAAGGAAATGAAGCTTTCAACTCAGGAGATTATGAAGAAGCAGTGATG TATTATACCAGGAGCATATCAGCGCTTCCCACTGTAGTTGCCTATAACAATCGAGCTCAAGCAGAAATC AAATTACAGAACTGGAATAGTGCTTTTCAGGATTGTGAAAAGGTCTTGGAGTTAGAACCTGGAAACGTA AAGGCTCTTCTGCGTCGTGCTACTACATATAAACATCAAAACAAGCTCCGGGAAGCTACAGAAGATTTG AGTAAAGTACTAGATGTTGAGCCTGATAATGATTTGGCCAAGAAAACCTTGTCAGAGGTTGAAAGAGAT CTGAAAAATTCTGAAGCTGCATCTGAGACTCAAACCAAAGGGAAAAGGATGGTTATTCAGGAAATAGAA AACTCCGAAGATGAAGAAGGAAAAAGCGGAAGAAAACATGAAGATGGCGGTGGAGATAAGAAGCCCGCG GAGCCGGCGGGAGCCGCGCGCGCCGCCCAGCCGTGCGTCATGGGCAACATCCAGAAGAAGCTGACTGGC AAAGCCGAAGGCGGCAAGCGGCCGGCAAGGGGCGCGCCGCAGCGGGGCCAGACCCCGGAGGCCGGCGCG GACAAGCGGAGCCCACGGCGGGCCTCTGCGGCGGCGGCGGCGGGCGGCGGCGCCACCGGGCATCCGGGC GGCGGGCAGGGCGCGGAGAACCCTGCCGGCCTGAAGAGCCAGGGCAACGAGCTGTTCCGAAGCGGGCAG TTCGCCGAGGCGGCCGGCAAGTACTCGGCGGCAATCGCGCTCCTGGAGCCAGCAGGAAGTGAAATTGCA GATGATCTAAGTATCTTATATTCAAATAGAGCAGCATGTTACCTAAAAGAAGGAAACTGCAGTGGCTGC ATTCAAGATTGTAACAGGGCTCTGGAACTTCATCCATTCTCTATGAAACCTCTTCTGAGGCGGGCGATG GCCTATGAAACTCTAGAGCAGTATGGGAAAGCTTATGTGGATTATAAAACAGTGTTGCAGATAGACTGT GGACTCCAGCTAGCAAATGACAGTGTTAACAGGCTATCAAGAATTTTAATGGAGCTGGATGGACCAAAT TGGCGGGAGAAGCTGTCACCTATTCCTGCTGTGCCTGCTTCTGTGCCACTGCAAGCTTGGCATCCGGCA AAAGAGATGATCTCAAAACAAGCAGGAGACTCCAGCAGCCATCGCCAGCAGGGCATCACAGATGAAAAA ACATTTAAAGCCCTTAAGGAAGAAGGAAATCAATGTGTAAATGACAAAAACTATAAAGACGCCCTCAGT AAATACAGCGAATGCTTAAAGATTAACAATAAGGAATGTGCCATATATACAAACAGAGCTCTCTGTTAC TTGAAGCTGTGCCAGTTTGAAGAAGCAAAGCAGGACTGTGATCAGGCACTTCAGCTAGCTGATGGGAAC GTGAAAGCCTTCTATAGACGAGCTCTGGCTCATAAAGGACTCAAGAATTATCAGAAAAGCTTAATTGAT CTCAATAAAGTTATCCTACTAGATCCAAGTATTATTGAGGCAAAGATGGAACTGGAAGAGGTAACTAGA CTCCTTAATCTTAAGGATAAGACAGCACCATTCAACAAAGAAAAGGAGAGAAGGAAAATTGAGATTCAA GAGGTGAATGAAGGCAAGGAGGAGCCTGGAAGACCTGCAGGGGAGGTCTCCATGGGATGCCTTGCTTCT GAGAAGGGAGGCAAAAGCAGCAGGTCACCAGAAGACCCTGAGAAACTTCCGATAGCCAAGCCTAATAAT GCCTATGAATTTGGTCAGATTATAAATGCTCTCAGTACCAGGAAGGATAAAGAAGCCTGTGCACATCTT TTAGCCATCACTGCACCAAAAGATTTGCCGATGTTTTTAAGTAACAAACTTGAAGGGGATACATTCCTT CTCCTCATTCAGTCTCTGAAAAATAATCTTATTGAAAAAGATCCCTCATTGGTGTATCAGCATCTTTTA TACCTGAGTAAAGCAGAAAGGTTTAAGATGATGTTGACACTAATTAGCAAGGGCCAAAAGGAGCTAATT GAACAGCTGTTTGAGGACCTTTCGGACACACCAAACAACCATTTTACTTTAGAAGATATACAGGCCCTA AAAAGGCAGTATGAGCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_003114 |
| Insert Size | 2781 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003114.4 |
| RefSeq Size | 3958 bp |
| RefSeq ORF | 2781 bp |
| Locus ID | 6674 |
| UniProt ID | Q07617 |
| Domains | TPR |
| MW | 103.6 kDa |
| Gene Summary | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217728 | SPAG1 (Myc-DDK-tagged)-Human sperm associated antigen 1 (SPAG1), transcript variant 1 |
CNY 6784.00 |
|
| RC217728L3 | Lenti ORF clone of Human sperm associated antigen 1 (SPAG1), transcript variant 1, Myc-DDK-tagged |
CNY 9030.00 |
|
| RC217728L4 | Lenti ORF clone of Human sperm associated antigen 1 (SPAG1), transcript variant 1, mGFP tagged |
CNY 9030.00 |
|
| RG217728 | SPAG1 (tGFP-tagged) - Human sperm associated antigen 1 (SPAG1), transcript variant 1 |
CNY 7890.00 |
