VAC14 (NM_018052) Human Untagged Clone
CAT＃: SC314683
VAC14 (untagged)-Human Vac14 homolog (S. cerevisiae) (VAC14)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ArPIKfyve; TAX1BP2; TRX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314683 representing NM_018052.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACCCCGAGAAGGATTTCGCGCCGCTCACGCCTAACATCGTGCGCGCCCTCAATGACAAGCTGTAC GAAAAGCGGAAGGTGGCAGCGCTGGAGATCGAGAAGCTGGTCCGGGAGTTCGTGGCCCAGAACAATACC GTGCAAATCAAGCATGTGATCCAGACCCTGTCCCAGGAGTTTGCCCTGTCTCAGCACCCCCACAGCCGG AAAGGGGGCCTCATCGGCCTGGCCGCCTGCTCCATCGCACTGGGCAAGGACTCAGGGCTCTACCTGAAG GAGCTGATCGAGCCAGTGCTGACCTGCTTCAATGATGCAGACAGCAGGCTGCGCTACTATGCCTGCGAG GCCCTCTACAACATCGTCAAGGTGGCCCGGGGCGCTGTGCTGCCCCACTTCAACGTGCTCTTTGACGGG CTGAGCAAGCTGGCAGCCGACCCAGACCCCAATGTGAAAAGCGGATCTGAGCTCCTAGACCGCCTTTTA AAGGACATTGTGACTGAGAGCAACAAGTTTGACCTGGTGAGCTTCATCCCCTTGTTGCGAGAGAGGATT TACTCCAACAACCAGTATGCCCGGCAGTTCATCATCTCCTGGATCCTGGTTCTGGAGTCGGTGCCAGAC ATTAACCTGCTGGATTACCTGCCGGAGATCCTGGATGGACTCTTCCAGATCCTGGGTGACAATGGCAAA GAGATTCGCAAAATGTGTGAGGTTGTTCTTGGAGAATTCTTAAAAGAAATTAAGAAGAACCCCTCCAGT GTGAAGTTTGCTGAGATGGCCAACATCCTGGTGATCCACTGCCAGACAACAGATGACCTCATCCAGCTG ACAGCCATGTGCTGGATGCGGGAGTTCATCCAGCTGGCGGGCCGCGTCATGCTGCCTTACTCCTCCGGG ATCCTGACTGCTGTCTTGCCCTGCTTGGCCTACGATGACCGCAAGAAAAGCATCAAAGAAGTGGCCAAC GTGTGCAACCAGAGCCTGATGAAGCTGGTCACCCCCGAGGACGACGAGCTGGATGAGCTGAGACCTGGG CAGAGGCAGGCAGAGCCCACCCCTGACGATGCCCTGCCAAAGCAGGAGGGCACAGCCAGTGGAGGTCCA GATGGTTCCTGTGACTCCAGCTTCAGTAGCGGCATCAGTGTCTTCACTGCAGCCAGCACTGAAAGAGCC CCAGTGACCCTTCACCTCGACGGGATCGTGCAGGTCCTAAACTGCCACCTCAGTGACACGGCCATTGGG ATGATGACCAGGATTGCAGTTCTCAAGTGGCTCTACCACCTCTACATCAAAACTCCTCGGAAGATGTTC CGGCACACGGACAGCCTCTTTCCCATCCTACTGCAGACGTTATCGGATGAATCGGATGAGGTGATCCTG AAGGACCTGGAGGTGCTGGCAGAAATCGCTTCCTCCCCCGCAGGCCAGACGGATGACCCAGGCCCCCTC GATGGCCCTGACCTCCAGGCCAGCCACTCAGAGCTCCAGGTGCCCACCCCTGGCAGAGCCGGCCTACTG AACACCTCTGGTACCAAAGGCTTAGAATGTTCTCCTTCAACTCCCACCATGAATTCTTACTTTTATAAG TTCATGATCAACCTTCTCAAGAGATTCAGCAGCGAACGGAAGCTCCTGGAGGTCAGAGGCCCTTTCATC ATCAGGCAGCTGTGCCTCCTGCTGAATGCGGAGAACATCTTCCACTCAATGGCAGACATCCTGCTGCGG GAGGAGGACCTCAAGTTCGCCTCGACCATGGTCCACGCCCTCAACACCATCCTGCTGACCTCCACAGAG CTCTTCCAGCTAAGGAACCAGCTGAAGGACCTGAAGACCCTGGAGAGCCAGAACCTGTTCTGCTGCCTG TACCGCTCCTGGTGCCACAACCCAGTCACCACGGTGTCCCTCTGCTTCCTCACCCAGAACTACCGGCAC GCCTATGACCTCATCCAGAAGTTTGGGGACCTGGAGGTCACCGTGGACTTCCTCGCAGAGGTGGACAAG CTGGTGCAGCTGATTGAGTGCCCCATCTTCACATATCTGCGCCTGCAGCTGCTGGACGTGAAGAACAAC CCCTACCTGATCAAGGCCCTCTACGGCCTGCTCATGCTCCTGCCGCAGAGCAGCGCCTTCCAGCTGCTC TCGCACCGGCTCCAGTGCGTGCCCAACCCTGAGCTGCTGCAGACCGAAGACAGTCTAAAGGCAGCCCCC AAGTCCCAGAAAGCTGACTCCCCTAGCATCGACTACGCAGAGCTGCTGCAGCACTTTGAGAAGGTCCAG AACAAGCACCTGGAAGTGCGGCACCAGCGGAGCGGGCGTGGGGACCACCTGGACCGGAGGGTTGTCCTC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_018052 |
| Insert Size | 2349 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018052.3 |
| RefSeq Size | 3107 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 55697 |
| UniProt ID | Q08AM6 |
| MW | 88 kDa |
| Gene Summary | This gene encodes a scaffold protein that is a component of the PIKfyve protein kinase complex. This complex is responsible for the synthesis of phosphatidylinositol 3,5-bisphosphate, an important component of cellular membranes, from phosphatidylinositol 3-phosphate. Mice lacking a functional copy of this gene exhibit severe neurodegeneration. Mutations in the human gene have been identified in patients with a childhood onset progressive neurological disorder characterized by impaired movement, dystonia, and striatal abnormalities. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214195 | VAC14 (Myc-DDK-tagged)-Human Vac14 homolog (S. cerevisiae) (VAC14) |
CNY 5728.00 |
|
| RC214195L1 | Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), Myc-DDK-tagged |
CNY 8128.00 |
|
| RC214195L2 | Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), mGFP tagged |
CNY 5890.00 |
|
| RC214195L3 | Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC214195L4 | Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), mGFP tagged |
CNY 5890.00 |
|
| RG214195 | VAC14 (tGFP-tagged) - Human Vac14 homolog (S. cerevisiae) (VAC14) |
CNY 4370.00 |
