NOL6 (NM_139235) Human Untagged Clone
CAT＃: SC314464
NOL6 (untagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma
CNY 13490.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bA311H10.1; NRAP; UTP22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314464 representing NM_139235.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGCCGGCGCCAGCTGGAGAGCAGCTTCGCGGAGCGACTGGAGAGCCAGAGGTGATGGAACCAGCC CTGGAAGGCACAGGCAAAGAGGGGAAGAAAGCATCCTCCAGGAAGCGTACATTGGCTGAACCTCCAGCG AAGGGCCTCCTGCAGCCAGTGAAGCTCAGCAGGGCAGAACTGTACAAGGAGCCTACCAATGAGGAGCTT AATCGCCTTCGGGAGACTGAGATCTTGTTCCACTCCAGCTTGCTTCGTTTACAGGTAGAGGAGCTACTA AAGGAAGTAAGGCTGTCAGAGAAGAAGAAGGATCGGATTGATGCCTTCCTACGGGAGGTCAACCAGCGG GTTGTGAGGGTGCCCTCAGTCCCTGAGACAGAGCTCACTGACCAGGCATGGCTCCCAGCTGGGGTTCGA GTGCCCCTCCACCAAGTGCCCTATGCCGTGAAGGGCTGTTTCCGCTTCCTGCCCCCAGCCCAGGTTACT GTTGTGGGCAGCTACCTTCTGGGCACCTGCATCCGACCAGACATCAATGTGGATGTGGCACTGACCATG CCCAGGGAAATCCTACAGGACAAGGACGGGCTGAACCAGCGCTACTTCCGCAAGCGTGCCCTCTACCTG GCCCACTTGGCTCACCACCTGGCCCAGGACCCCCTCTTTGGCAGTGTTTGCTTCTCCTACACAAATGGC TGCCACCTGAAACCCTCACTGTTGCTGCGGCCGCGTGGAAAGGATGAGCGCCTGGTCACTGTACGTCTG CATCCGTGCCCTCCACCTGACTTCTTCCGCCCGTGCCGCTTGCTGCCAACCAAGAACAATGTGCGCTCT GCCTGGTACCGAGGGCAGAGTCCTGCAGGGGATGGTAGCCCAGAGCCTCCTACCCCCCGCTATAACACA TGGGTCCTGCAAGATACAGTTCTCGAGTCCCATTTGCAGCTGCTGTCAACCATTCTGAGTTCAGCCCAG GGCCTGAAGGATGGCGTGGCACTTCTGAAGGTCTGGCTGCGGCAGCGGGAGCTGGACAAGGGCCAGGGT GGGTTTACTGGGTTCCTTGTCTCCATGCTGGTTGTCTTCCTTGTGTCTACACGCAAGATCCATACCACC ATGAGTGGCTACCAGGTCCTGAGAAGTGTCTTGCAGTTTCTGGCCACTACAGACCTGACAGTCAACGGG ATCAGTTTATGTCTCAGCTCAGATCCCTCTTTGCCGGCCCTGGCTGACTTCCACCAGGCCTTCTCCGTT GTCTTCCTGGATTCCTCAGGCCATCTCAACCTCTGTGCTGATGTCACTGCCTCTACTTACCACCAGGTA CAGCATGAGGCACGGCTGTCTATGATGTTGCTGGACAGCAGAGCTGACGACGGGTTCCACCTGCTGTTG ATGACTCCCAAACCCATGATCCGGGCTTTTGACCATGTCCTGCATCTCCGTCCACTGAGTCGCCTGCAG GCAGCGTGCCACCGGCTGAAGCTCTGGCCAGAGCTGCAGGACAATGGTGGGGACTATGTCTCAGCTGCT TTGGGCCCCCTGACCACCCTCCTGGAGCAGGGCCTGGGGGCTCGGCTGAACCTGCTGGCTCACTCTCGA CCCCCAGTCCCAGAGTGGGACATCAGCCAAGATCCACCAAAGCACAAAGACTCTGGGACCCTGACCCTG GGACTCCTTCTCCGGCCTGAGGGACTGACCAGCGTCCTTGAGCTGGGTCCAGAGGCAGACCAGCCTGAG GCTGCTAAATTCCGCCAGTTCTGGGGATCCCGCTCGGAGCTTCGGCGTTTCCAGGACGGAGCCATTCGG GAAGCTGTGGTCTGGGAGGCAGCCTCTATGTCCCAGAAGCGCCTTATTCCCCACCAGGTGGTCACCCAC CTCTTGGCACTCCATGCTGACATCCCAGAAACCTGTGTCCACTATGTGGGGGGCCCCCTGGATGCACTT ATCCAAGGCCTGAAAGAGGCCTCCAGCACAAAGGGGCGCATGGTGATGTCTCGAGGTGGGGAGCTAGTA ATGGTGCCCAATGTTGAAGCAATCCTGGAGGACTTTGCTGTGCTGGGTGAAGGCCTGGTGCAGACTGTG GAGGCCCGAAGTGAGAGGTGGACTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_139235 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139235.3 |
| RefSeq Size | 3554 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 65083 |
| UniProt ID | Q9H6R4 |
| Protein Families | Druggable Genome |
| MW | 77.6 kDa |
| Gene Summary | The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (gamma) lacks nine exons in the 3' region compared to variant alpha. The encoded isoform (gamma), also known as the short isoform, has a unique C-terminus compared to the alpha isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224100 | NOL6 (Myc-DDK-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma |
CNY 5120.00 |
|
| RC224100L3 | Lenti-ORF clone of NOL6 (Myc-DDK-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma |
CNY 9310.00 |
|
| RC224100L4 | Lenti-ORF clone of NOL6 (mGFP-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma |
CNY 9310.00 |
|
| RG224100 | NOL6 (tGFP-tagged) - Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma |
CNY 8170.00 |
