PAN3 (NM_175854) Human Untagged Clone
CAT＃: SC314436
PAN3 (untagged)-Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3)
CNY 17100.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314436 representing NM_175854.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACAGTGGCGGCGGCCTCCCGCCCCCCTCGGCCGCCGCCTCCCCTTCCTCCTCCTCGCTGGCGGCG GCGGTGGCGGTGGTGGCCCCGCCGGGGGTCGGGGGTGTCCCCGGCGGGGCGGCGGTAGGAGTGAAGCTG AAGTACTGCCGCTACTACGCTAAGGATAAGACTTGCTTCTACGGGGAGGAGTGTCAGTTCCTGCATGAG GACCCTGCCGCCGGGGCTGCCCCGGGCCTCGGCCTCCATAGCAACAGCGTCCCCCTGGCTCTGGCTGGT GCACCCGTGGCCGGCTTTCCGCCGGGAGCCGTCGCGGGCGGGGGAGCTGGGCCGCCCCCCGGGCCCAAG AAGCCGGACCTGGGGGACCCGGGGACCGGAGCCGCAGCCGGCGGAGGAGGCAGTAGCGGGGGACTCGAT GGACCGCGGCTGGCAATTCCAGGAATGGATGGAGGTGCTTTAACTGATACAAGTCTCACAGATTCCTAT TTTAGCACCAGCTTTATTGGAGTCAATGGATTTGGAAGCCCTGTAGAAACAAAATATCCCCTGATGCAG AGAATGACTAATAGTAGCAGCTCCCCAAGCCTTCTAAATGACAGTGCCAAGCCATATTCAGCCCATGAT CCTCTAACATCACCTGCTTCATCCTTGTTTAATGACTTTGGTGCCCTCAACATCTCTCAGAGACGAAAG CCAAGGAAGTATCGCCTGGGGATGCTGGAGGAGAGGCTAGTTCCGATGGGATCAAAGGCACGAAAAGCA AAGAACCCTATTGGCTGCCTTGCTGACAGGTGTAAATCAGGAGTACCCATCAATATGGTTTGGTGGAAC AGAGTCACAGAAAACAATTTACAGACACCAAATCCTACTGCAAGCGAGTTTATTCCTAAAGGAGGATCA ACCTCCAGGCTGAGTAACGTGTCCCAGTCAAATATGTCTGCCTTCTCTCAAGTTTTCTCTCACCCATCC ATGGGAAGCCCTGCTACTGCTGGATTAGCGCCAGGAATGTCGTTGTCTGCTGGGTCTTCCCCTCTTCAT TCCCCCAAGATTACTCCACATACTTCTCCTGCTCCCAGAAGAAGAAGTCACACTCCAAATCCAGCAAGT TACATGGTGCCTTCTAGTGCCTCTACATCTGTTAATAATCCTGTTTCTCAGACTCCGTCTTCTGGTCAG GTGATCCAAAAGGAAACTGTTGGTGGGACGACTTACTTCTATACAGACACAACTCCAGCACCTTTGACT GGAATGGTGTTTCCAAACTATCATATTTATCCTCCAACTGCACCTCACGTTGCTTATATGCAACCGAAA GCAAACGCACCTTCCTTCTTCATGGCTGATGAACTCCGACAGGAGCTGATCAACAGACATTTAATAACA ATGGCTCAAATTGATCAAGCAGATATGCCAGCAGTTCCTACAGAGGTTGACAGCTACCATAGCCTATTC CCTCTAGAACCACTGCCACCTCCCAACCGGATACAGAAATCAAGTAATTTTGGATATATTACATCTTGC TACAAAGCTGTAAACAGCAAAGATGATCTGCCATATTGCCTTCGGAGGATACATGGTTTTCGTCTTGTT AACACAAAGTGCATGGTGTTGGTCGACATGTGGAAGAAAATTCAACACTCAAATATCGTAACTTTGCGT GAAGTATTTACCACTAAAGCATTTGCTGAGCCCTCTCTTGTGTTTGCATATGATTTCCATGCTGGAGGA GAAACTATGATGAGCAGACACTTTAATGACCCTAATGCTGATGCCTACTTCACCAAGAGAAAGTGGGGT CAGCACGAGGGACCATTGCCCAGGCAGCATGCTGGATTATTGCCAGAATCTCTTATTTGGGCATATATT GTCCAACTAAGTTCTGCATTGCGTACCATTCATACAGCAGGTTTGGCATGTCGAGTTATGGATCCAACA AAGATTCTGATAACTGGCAAAACAAGGTTGCGAGTAAATTGTGTTGGAGTTTTTGATGTTTTAACATTT GATAACAGTCAAAATAATAATCCATTGGCATTAATGGCCCAGTACCAGCAAGCAGATCTGATATCATTA GGAAAAGTTGTGTTGGCTTTGGCTTGCAACTCTTTGGCAGGAATTCAGCGAGAGAATTTACAGAAAGCC ATGGAACTGGTGACAATCAACTATTCCTCTGACCTGAAGAATCTGATTTTGTATTTGTTGACTGACCAA AACAGGATGCGAAGTGTAAATGACATCATGCCCATGATTGGTGCTCGATTTTATACTCAATTGGATGCT GCTCAAATGAGAAATGATGTCATAGAGGAAGACCTTGCAAAGGAGGTTCAAAATGGAAGACTGTTTAGG CTCCTAGCAAAATTGGGAACAATCAATGAGAGGCCGGAGTTTCAGAAGGATCCCACTTGGTCAGAGACT GGAGACCGTTATCTGTTGAAACTCTTTAGGGATCATCTTTTTCATCAGGTGACAGAAGCAGGTGCTCCC TGGATTGACCTCAGTCATATAATTTCTTGTCTTAACAAGCTAGATGCTGGTGTGCCAGAAAAAATAAGC CTGATTTCCAGAGATGAGAAGAGTGTACTTGTGGTGACCTACAGTGACTTAAAGCGCTGCTTTGAAAAT ACTTTTCAAGAACTGATTGCAGCTGCAAATGGTCAGTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_175854 |
| Insert Size | 2664 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175854.7 |
| RefSeq Size | 5643 bp |
| RefSeq ORF | 2664 bp |
| Locus ID | 255967 |
| UniProt ID | Q58A45 |
| Protein Families | Druggable Genome |
| MW | 95.6 kDa |
| Gene Summary | Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218027 | PAN3 (Myc-DDK-tagged)-Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3) |
CNY 5032.00 |
|
| RC218027L3 | Lenti ORF clone of Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3), Myc-DDK-tagged |
CNY 11310.00 |
|
| RC218027L4 | Lenti ORF clone of Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3), mGFP tagged |
CNY 11310.00 |
|
| RG218027 | PAN3 (tGFP-tagged) - Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3) |
CNY 10360.00 |
