SPTY2D1 (NM_194285) Human Untagged Clone
CAT＃: SC314427
SPTY2D1 (untagged)-Human SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) (SPTY2D1)
CNY 13210.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Spt2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314427 representing NM_194285.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACTTCAGAGAAATTCTCATGATAGCTTCCAAGGGACAAGGTGTCAACAATGTGCCGAAAAGGTAT AGTTTGGCAGTGGGGCCTCCAAAAAAAGACCCAAAAGTTAAAGGTGTCCAATCAGCAGCTGTACAAGCT TTTCTTAAAAGGAAAGAAGAGGAGCTGAGACGAAAAGCCTTAGAGGAGAAAAGGAGAAAAGAGGAACTA GTGAAAAAGCGAATTGAGCTCAAACATGACAAGAAAGCAAGAGCTATGGCCAAGAGGACAAAGGATAAT TTCCATGGTTACAATGGGATTCCTATTGAGGAAAAGTCAAAGAAGAGGCAGGCAACAGAAAGCCATACC AGCCAAGGAACCGACCGAGAGTATGAAATGGAAGAAGAGAATGAATTCCTCGAGTACAATCACGCAGAG TCAGAGCAGGAGTATGAGGAAGAGCAAGAACCTCCCAAAGTTGAAAGCAAACCAAAGGTCCCCCTTAAA AGTGCCCCACCACCCATGAACTTCACTGATTTACTCAGGCTGGCTGAGAAAAAGCAGTTTGAACCAGTG GAAATCAAGGTAGTGAAGAAATCAGAAGAGCGACCTATGACCGCAGAAGAACTTAGGGAGCGAGAATTC CTTGAACGAAAGCATAGGAGAAAAAAACTTGAGACAGATGGAAAACTACCTCCAACTGTGTCCAAAAAG GCACCCTCTCAGAAAGAAAGTGTGGGCACAAAACTTAGCAAAGGTTCTGGAGACAGGCATCCTTCTTCC AAAGGAATGCCCCTTCCTCATGCTGAGAAGAAATCAAGACCCAGCATGGCCAATGAGAAACACCTCGCT TTGTCTTCATCCAAATCCATGCCAGGAGAGAGGATCAAGGCAGGATCTGGCAATAGCTCCCAACCCTCA CTTCGTGAGGGCCACGACAAACCTGTTTTTAATGGAGCTGGAAAGCCTCATTCCAGCACCTCTTCACCA AGTGTCCCAAAGACTTCTGCTAGCAGGACTCAGAAATCTGCTGTTGAGCACAAAGCCAAAAAATCTCTG TCCCATCCTAGCCATTCCAGGCCTGGGCCCATGGTCACCCCACACAATAAGGCTAAGAGTCCAGGTGTC AGGCAGCCAGGCAGCAGCTCTAGCTCAGCCCCTGGGCAGCCCAGCACAGGGGTTGCTCGACCCACAGTT AGTTCTGGCCCTGTGCCTAGGCGCCAGAATGGCAGCTCCAGCTCAGGACCTGAGCGATCAATCAGTGGG TCCAAGAAGCCAACCAATGACTCAAATCCCTCTAGGCGGACAGTCAGTGGTACATGTGGCCCTGGACAA CCTGCAAGCAGCTCAGGTGGCCCTGGGCGACCCATCAGTGGTTCAGTTAGTTCTGCAAGACCCTTGGGC AGCTCTCGTGGCCCTGGCCGGCCTGTGAGCAGTCCACATGAACTTCGACGACCAGTGAGTGGCTTGGGC CCCCCGGGGCGGTCTGTCAGTGGCCCTGGGAGATCCATAAGTGGCTCAATTCCAGCTGGACGGACTGTC AGTAATTCAGTCCCAGGAAGACCAGTGAGCAGCTTGGGACCTGGGCAAACAGTTAGTAGCTCAGGTCCC ACTATAAAGCCTAAGTGCACTGTTGTCTCCGAAACAATTTCTTCCAAGAATATCATTAGCCGGTCCAGC AATGGACAGATGAATGGAATGAAGCCTCCCCTATCTGGCTACAGAGCTGCCCAAGGTCCTCAAAGGCTT CCCTTCCCTACTGGTTACAAAAGGCAGCGAGAATATGAAGAGGAAGATGACGATGATGATGAATACGAC TCTGAAATGGAAGATTTTATTGAAGATGAAGGAGAGCCTCAGGAAGAAATATCCAAGCACATTAGAGAA ATCTTTGGTTATGACCGAAAAAAATACAAAGATGAAAGTGATTATGCCTTACGTTACATGGAAAGTAGT TGGAAAGAGCAGCAGAAGGAAGAAGCAAAGAGCTTAAGACTGGGTATGCAAGAGGACTTAGAGGAAATG AGACGTGAAGAAGAAGAAATGCAACGTCGAAGGGCCAAGAAGCTGAAGAGGCGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_194285 |
| Insert Size | 2058 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_194285.2 |
| RefSeq Size | 5752 bp |
| RefSeq ORF | 2058 bp |
| Locus ID | 144108 |
| UniProt ID | Q68D10 |
| MW | 75.6 kDa |
| Gene Summary | Histone chaperone that stabilizes pre-existing histone tetramers and regulates replication-independent histone exchange on chromatin (PubMed:26109053). Required for normal chromatin refolding in the coding region of transcribed genes, and for the suppression of spurious transcription (PubMed:26109053). Binds DNA and histones and promotes nucleosome assembly (in vitro) (PubMed:23378026, PubMed:26109053). Facilitates formation of tetrameric histone complexes containing histone H3 and H4 (PubMed:26109053). Modulates RNA polymerase 1-mediated transcription (By similarity). Binds DNA, with a preference for branched DNA species, such as Y-form DNA and Holliday junction DNA (PubMed:23378026).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223508 | SPTY2D1 (Myc-DDK-tagged)-Human SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) (SPTY2D1) |
CNY 5016.00 |
|
| RC223508L3 | Lenti ORF clone of Human SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) (SPTY2D1), Myc-DDK-tagged |
CNY 7700.00 |
|
| RC223508L4 | Lenti ORF clone of Human SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) (SPTY2D1), mGFP tagged |
CNY 7700.00 |
|
| RG223508 | SPTY2D1 (tGFP-tagged) - Human SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) (SPTY2D1) |
CNY 6370.00 |
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