SPATA13 (NM_153023) Human Untagged Clone
CAT＃: SC314333
SPATA13 (untagged)-Human spermatogenesis associated 13 (SPATA13), transcript variant 2
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARHGEF29; ASEF2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314333 representing NM_153023.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTTCTGCCAGCCCTGAAGACCAGAATGCTCCAGTGGGCTGCCCCAAAGGAGCCCGGAGAAGGCGC CCCATTTCCGTGATAGGTGGGGTCAGCTTGTATGGGACCAACCAGACGGAGGAACTGGACAATCTTCTG ACCCAACCGGCTTCCAGGCCGCCCATGCCTGCTCACCAGGTGCCACCCTACAAGGCTGTGTCGGCCCGG TTCCGGCCCTTCACATTCTCCCAGAGCACCCCCATTGGGTTGGACCGTGTGGGACGCCGGCGGCAGATG AGAGCATCCAACGTTTCTTCAGATGGAGGTACTGAGCCCTCTGCCTTAGTGGATGACAACGGTAGTGAG GAGGACTTCAGCTATGAAGACCTCTGCCAGGCCAGCCCTCGGTACCTGCAGCCCGGCGGGGAGCAGCTG GCCATCAATGAGCTGATCAGTGATGGCAACGTGGTCTGCGCAGAAGCCCTGTGGGACCATGTGACCATG GATGACCAGGAACTGGGCTTCAAAGCCGGGGATGTCATCCAGGTTCTGGAAGCCTCCAACAAGGACTGG TGGTGGGGCCGCAGTGAAGATAAGGAAGCCTGGTTCCCCGCGAGCTTCGTCAGATTGCGAGTGAATCAG GAAGAGCTGTCGGAAAACTCCAGCAGCACCCCCAGTGAGGAGCAGGACGAGGAGGCCAGCCAGAGCCGC CACAGACACTGTGAGAACAAGCAGCAGATGCGGACCAACGTCATCCGGGAGATCATGGACACCGAGCGG GTGTACATCAAACACCTCAGGGACATCTGTGAGGGCTATATCCGACAGTGCCGCAAGCACACAGGAATG TTCACCGTTGCGCAGCTAGCCACTATTTTTGGAAACATTGAAGATATTTACAAATTCCAAAGAAAGTTT CTGAAAGACCTTGAGAAACAGTACAACAAAGAGGAACCTCACTTAAGTGAAATAGGATCTTGCTTTCTT CAAAATCAAGAGGGCTTTGCCATCTATTCCGAGTACTGCAACAACCACCCGGGCGCCTGCCTGGAGCTC GCCAACCTCATGAAGCAGGGCAAGTACAGACATTTCTTTGAAGCCTGCCGCCTGCTGCAGCAGATGATT GACATCGCCATCGACGGGTTCCTGCTCACACCAGTGCAGAAGATCTGCAAATACCCGCTGCAGCTGGCC GAGCTGCTCAAGTATACCACACAGGAACACGGTGATTACAGCAACATAAAGGCAGCATATGAGGCCATG AAGAATGTGGCCTGTCTGATCAACGAGCGCAAGCGCAAGCTGGAGAGCATCGACAAGATAGCTCGCTGG CAGGTGTCTATCGTGGGCTGGGAGGGACTGGATATCTTAGACCGAAGCTCAGAATTGATTCATTCTGGG GAGCTGACCAAAATCACTAAGCAAGGCAAAAGCCAGCAGCGGACGTTCTTCCTGTTTGACCACCAGCTG GTGTCCTGCAAGAAGGACCTGCTGCGCAGGGACATGCTGTACTACAAGGGCCGGCTGGACATGGATGAG ATGGAGCTTGTGGACCTGGGGGATGGGCGCGACAAGGACTGCAACCTCAGCGTGAAAAATGCCTTCAAG CTCGTCAGTAGGACCACAGACGAGGTTTATTTGTTTTGTGCCAAAAAACAAGAAGACAAGGCGAGGTGG CTGCAGGCCTGTGCAGATGAAAGGAGGCGGGTGCAAGAGGACAAGGAGATGGGAATGGAAATTTCAGAA AACCAGAAGAAACTTGCCATGTTAAATGCTCAAAAGGCAGGACATGGAAAGTCAAAAGGCTACAACAGG TGCCCTGTGGCCCCACCGCACCAGGGCCTGCACCCCATCCACCAGCGCCACATCACTATGCCCACAAGC GTCCCCCAGCAGCAGGTCTTTGGCCTGGCGGAACCCAAGAGGAAGTCCTCGCTCTTCTGGCACACCTTC AACAGGCTCACCCCCTTCCGGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153023 |
| Insert Size | 1959 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153023.3 |
| RefSeq Size | 6699 bp |
| RefSeq ORF | 1959 bp |
| Locus ID | 221178 |
| UniProt ID | Q96N96 |
| Domains | RhoGEF, SH3, PH |
| MW | 74.8 kDa |
| Gene Summary | Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' reading frame, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219712 | SPATA13 (Myc-DDK-tagged)-Human spermatogenesis associated 13 (SPATA13), transcript variant 2 |
CNY 4856.00 |
|
| RC219712L1 | Lenti ORF clone of Human spermatogenesis associated 13 (SPATA13), transcript variant 2, Myc-DDK-tagged |
CNY 7256.00 |
|
| RC219712L2 | Lenti ORF clone of Human spermatogenesis associated 13 (SPATA13), transcript variant 2, mGFP tagged |
CNY 6460.00 |
|
| RC219712L3 | Lenti ORF clone of Human spermatogenesis associated 13 (SPATA13), transcript variant 2, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC219712L4 | Lenti ORF clone of Human spermatogenesis associated 13 (SPATA13), transcript variant 2, mGFP tagged |
CNY 6460.00 |
|
| RG219712 | SPATA13 (tGFP-tagged) - Human spermatogenesis associated 13 (SPATA13), transcript variant 2 |
CNY 5040.00 |
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