ZBTB1 (NM_014950) Human Untagged Clone
CAT＃: SC314314
ZBTB1 (untagged)-Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2
CNY 10360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ZNF909 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314314 representing NM_014950.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAAAGCCCAGCCACAGCAGCTATGTCCTTCAGCAGCTAAACAACCAAAGAGAATGGGGTTTTCTC TGTGACTGCTGTATTGCAATTGATGACATTTACTTTCAAGCACACAAGGCAGTTCTAGCTGCCTGTAGC TCCTATTTTAGAATGTTTTTCATGAACCATCAGCATAGTACTGCACAACTGAATCTCAGCAACATGAAA ATTAGTGCAGAATGTTTTGATCTCATTTTGCAGTTTATGTATTTAGGAAAAATTATGACAGCTCCCTCC AGTTTTGAGCAGTTTAAAGTGGCAATGAACTACCTACAGCTATACAATGTTCCTGACTGTTTAGAAGAC ATCCAGGATGCAGATTGTTCTAGTTCAAAATGTTCCTCTTCTGCTTCCAGCAAACAGAACAGCAAAATG ATATTTGGGGTAAGAATGTATGAAGATACTGTGGCTCGAAATGGCAATGAAGCCAACAGGTGGTGTGCA GAGCCAAGTTCAACGGTAAATACACCACATAATAGAGAGGCTGATGAAGAGTCTTTACAATTAGGTAAT TTTCCTGAGCCACTATTTGATGTATGTAAAAAAAGTTCCGTGTCCAAATTATCTACTCCAAAAGAACGT GTGTCAAGACGCTTTGGGCGGAGTTTTACCTGTGATAGCTGTGGATTTGGCTTTAGCTGTGAAAAATTA TTAGATGAGCATGTGCTAACCTGTACTAACAGACATTTATACCAAAACACAAGATCTTACCATAGAATA GTAGATATTAGAGATGGAAAAGACAGTAACATCAAAGCTGAATTTGGTGAAAAAGATTCTTCCAAAACA TTTTCTGCACAGACGGACAAATACAGAGGAGACACAAGCCAGGCTGCTGATGATTCAGCTTCAACCACT GGAAGCAGAAAAAGTAGCACAGTGGAGTCTGAAATAGCAAGCGAAGAGAAAAGCAGAGCTGCTGAGAGG AAAAGGATTATTATTAAGATGGAGCCAGAAGATATTCCTACAGATGAACTGAAAGACTTTAACATTATT AAAGTTACTGATAAAGACTGTAATGAATCCACTGACAATGATGAATTAGAAGATGAACCTGAAGAGCCA TTTTATAGATACTATGTTGAAGAAGATGTCAGCATAAAAAAAAGTGGTAGGAAAACTCTAAAACCTCGA ATGTCAGTAAGTGCTGATGAAAGAGGTGGTTTAGAGAATATGAGGCCCCCTAACAACAGCAGTCCAGTA CAAGAGGATGCTGAAAATGCATCTTGTGAGCTGTGTGGACTTACAATAACCGAGGAGGACCTGTCATCT CATTACTTAGCCAAACACATTGAAAATATCTGTGCATGTGGTAAATGTGGACAAATACTTGTAAAGGGT AGGCAGCTTCAGGAACATGCTCAACGATGTGGCGAGCCCCAAGATCTGACCATGAATGGGTTAGGAAAT ACTGAGGAGAAAATGGACTTGGAAGAGAATCCTGATGAGCAGTCCGAAATAAGAGATATGTTTGTTGAA ATGCTGGATGATTTTAGGGACAATCATTACCAGATAAACAGTATCCAAAAAAAGCAGTTATTTAAACAT TCTGCCTGCCCTTTTCGATGTCCTAATTGTGGCCAGCGTTTTGAAACTGAAAATCTAGTGGTTGAACAT ATGTCTAGCTGCTTAGATCAAGATATGTTTAAGAGTGCCATCATGGAAGAAAATGAAAGAGATCACAGA CGAAAGCATTTTTGTAATCTGTGTGGAAAAGGATTTTATCAGCGGTGTCACTTAAGAGAACACTATACT GTTCATACTAAGGAAAAACAGTTTGTTTGTCAAACATGTGGAAAGCAGTTTTTAAGAGAGCGTCAGTTG CGACTGCACAATGATATGCACAAAGGCATGGCCAGTGGTGAAATAGGGCCTTCTAAACCTGTGGAGAAG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014950 |
| Insert Size | 1935 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014950.2 |
| RefSeq Size | 4119 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 22890 |
| UniProt ID | Q9Y2K1 |
| Domains | BTB, zf-C2H2 |
| Protein Families | Transcription Factors |
| MW | 73.7 kDa |
| Gene Summary | Acts as a transcriptional repressor (PubMed:20797634). Represses cAMP-responsive element (CRE)-mediated transcriptional activation (PubMed:21706167). In addition, has a role in translesion DNA synthesis. Requires for UV-inducible RAD18 loading, PCNA monoubiquitination, POLH recruitment to replication factories and efficient translesion DNA synthesis (PubMed:24657165). Plays a key role in the transcriptional regulation of T lymphocyte development (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains a distinct 3' coding region and 3' UTR, compared to variant 1. This results in a shorter isoform (2) with a distinct C-terminus, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219510 | ZBTB1 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2 |
CNY 4792.00 |
|
| RC219510L3 | Lenti ORF clone of Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC219510L4 | Lenti ORF clone of Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2, mGFP tagged |
CNY 6460.00 |
|
| RG219510 | ZBTB1 (tGFP-tagged) - Human zinc finger and BTB domain containing 1 (ZBTB1), transcript variant 2 |
CNY 5040.00 |
