RNF157 (AK091467) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK091467, the custom clone sequence may differ by one or more nucleotides ATGGGAGGAGAGAAGTTTGACTCAACTCATCCTGAAGGTTACCTGTTTGGAGAGAACAGC GATCTGAACTTTCTGGGGAACAGACCAGTTGTGTTTCCTTACGCCGCCCCACCTCCCCAA GAACCCGTGAAGACTCTGAGAAGCCTGGTCAATATCCGAAAGGACACACTGAGGCTCGTC AAATGTGCTGAGGAAGTGAAGAGCCCTGGAGAAGAGGCCAGTAAAGCTAAAGTCCACTAC AATGTTGAGTTCACCTTTGACACAGATGCTCGGGTAGCCATCACCATCTATTACCAGGCC ACGGAAGAGTTCCAGAATGGTATTGCCAGCTACATTCCCAAAGACAACAGCCTCCAGTCG GAGACTGTGCAGTACAAGCGAGGAGTGTGTCAGCAGTTCTGCCTGCCCTCCCACACCGTG GATCCCTCCGAGTGGGCCGAAGAGGAGCTTGGCTTTGATTTAGACCGAGAAGTTTACCCT CTAGTGGTACATGCCGTGGTGGATGAAGGAGACGAGTATTTTGGCCATTGCCATGTACTG CTGGGTACTTTTGAGAAGCACACAGATGGAACTTTCTGTGTCAAGCCCCTCAAACAGAAA CAAGTAGTAGACGGGGTCAGCTACCTCCTTCAGGAGATCTATGGAATTGAAAACAAGTAC AACACACAAGATTCTAAGGTGGCTGAAGACGAAGTGAGTGATAACAGTGCCGAGTGTGTG GTGTGTCTCTCGGATGTCCGGGACACCTTGATTCTGCCCTGTCGCCACCTCTGCCTCTGT AACACCTGTGCAGACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGACTGCCC TTCCGGGCACTGCTTCAGATCCGAGCCATGAGGAAAAAATTGGGCCCCTTGTCCCCAACC AGCTTTAACCCCATCATCTCATCCCAGACATCTGACTCTGAAGAGCATCCATCCTCAGAG AATATTCCACCAGGCTATGAAGTAGTATCTCTTCTGGAGGCCCTCAACGGGCCCCTCACC CCGTCCCCAGCAGTTCCTCCACTTCACGTGCTTGGAGATGGCCACCTCTCAGGAATGCTC CCTTCATATGGCAGTGATGGCCACCTGCCCCCCGTCAGGACGATCTCGCCTCTTGACCGC CTGTCTGACAGCAGCAGTCAGGGACTCAAACTCAAAAAGAGTCTCTCCAAATCCACTTCC CAAAACTCTTCCGTGCTGCATGAAGAGGAAGATGAGCATTCCTGCAGCGAGTCGGAGACA CAGCTCTCTCAGAGACCGTCGGTTCAGCATCTCGGAGAGGAATGTGGTGTGACTCCAGAA AGTGAGAATCTCACCTTGTCGTCATCTGGAGCTATTGACCAGTCGTCTTGCACAGGGACG CCTCTGTCATCCACTATTTCCTCCCCAGAAGGCCCTGCCAGCAGCAGCTTGGCCCAGTCT GTCATGTCCATGGCATCCTCCCAGATCAGCACTGACACCGTCTCCTCCATGTCTGGCTCC TACATCGCCCCTGGCACTGAAGAGGAGGGAGAGGCTCTCTCTTCCCCCCAGCCTGCCAGC AGGGCCCCCTCAGAAGAAGGAGAGGGAAATGATGTTATAGAGGAAGAGGATGGATCACCC ACGCAGGAAGGCCAGAGGACGTGCGCATTTCTAGGTATGGAGTGTGACAATAACAATGAC TTTGACATCGCAAGCGTGAAAGCACTGGACAATAAGCTGTGCTCTGAGGTCTGCTTACCT GGTGCCTGGCAGGCTGATGACAATGCCGTCAGTCGGAATGCCCAGCGCCGGCGCTTGTCA TCCAGCAGCCTGGAGGACTCTGAGACGAGGCCCTGTGTGTGGGGCCCTTTGGCTGTC
Restriction Sites	Please inquire
ACCN	AK091467
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091467.1, BAC03669.1
RefSeq Size	2821 bp
RefSeq ORF	1860 bp
Locus ID	114804
Domains	RING
Protein Families	Druggable Genome
Gene Summary	E3 ubiquitin ligase that ubiquitinates APBB1 for its degradation by the proteasome and thus prevents apoptosis and promotes survival of neurons (PubMed:25342469). Has a dual role in neurons as it is also required for dendrite growth and maintenance for which its ligase activity is not critical (PubMed:25342469). May act as a scaffold molecule to regulate this process (PubMed:25342469). Acts as a downstream effector of the interconnected PI3K and MAPK signaling pathways and thus participates in the regulation of the cell cycle (PubMed:28655764).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.