Sperm Flagellar 2 (SPEF2) (NM_144722) Human Untagged Clone
CAT＃: SC313797
SPEF2 (untagged)-Human sperm flagellar 2 (SPEF2), transcript variant 2
CNY 8270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT122; KPL2; SPGF43 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC313797 representing NM_144722.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGAGATCCTGTGCCAGTGGCTCAACAAGGAGTTGAAGGTGTCCCGGACCGTGAGTCCCAAGTCA TTTGCAAAGGCATTTTCCAGTGGCTATCTACTTGGAGAAGTTCTACACAAGTTTGAACTTCAGGATGAT TTTTCAGAATTTTTGGACAGCAGGGTTTCAAGTGCCAAACTTAATAATTTTTCTCGCTTGGAGCCAACA CTTAACCTTCTGGGTGTGCAGTTTGATCAGAATGTGGCCCATGGCATCATCACAGAAAAGCCTGGGGTG GCAACAAAGCTGTTATATCAATTGTACATTGCTCTTCAGAAAAAGAAGAAAAGTGGACTGACTGGAGTG GAGATGCAAACCATGCAACGTCTGACAAATTTAAGACTTCAAAACATGAAAAGTGATACTTTTCAAGAG AGACTTAGACACATGATACCACGTCAAACTGATTTCAATCTGATGCGGATTACATACAGGTTTCAAGAA AAATATAAACACGTGAAAGAAGATCTTGCCCATTTGCATTTTGAGAAACTTGAAAGATTTCAAAAACTC AAGGAAGAGCAAAGATGTTTTGATATTGAAAAGCAATACTTAAACAGAAGACGACAAAATGAAATAATG GCCAAAATCCAAGCAGCTATTATACAGATTCCTAAACCTGCATCAAATCGTACTTTGAAAGCACTCGAG GCCCAAAAAATGATGAAAAAGAAAAAAGAGGCAGAAGATGTGGCTGATGAAATTAAGAAGTTCGAAGCA TTAATAAAAAAGGATCTCCAAGCAAAAGAAAGTGCATCCAAGACTTCTTTAGATACAGCAGGCCAGACA ACCACCGATTTGTTAAATACTTACTCAGATGACGAGTACATTAAAAAAATCCAGAAGCGCCTTGAAGAA GATGCTTTTGCACGAGAGCAAAGGGAGAAAAGACGGCGGAAATTGTTAATGGACCAGTTAATAGCCCAC GAAGCACAAGAGGAGGCTTATCGGGAGGAACAGCTGATTAACCGGCTGATGCGGCAGTCCCAGCAGGAG CGCAGGATTGCCGTGCAGCTCATGCATGTTCGGCATGAAAAGGAAGTTTTATGGCAAAACAGAATTTTC AGAGAAAAACAACATGAGGAAAGACGACTTAAAGATTTCCAGGATGCTCTTGATCGAGAAGCGGCTTTG GCAAAACAAGCCAAGATTGACTTTGAAGAACAATTCCTTAAAGAAAAGAGATTTCATGATCAGATTGCT GTGGAAAGAGCTCAAGCTCGTTATGAAAAGCATTATTCAGTATGTGCAGAAATTTTGGATCAAATAGTT GATTTGTCCACTAAAGTGGCAGACTATCGAATGTTGACAAATAATCTGATTCCGTATAAGTTGATGCAT GATTGGAAGGAACTATTTTTTAATGCAAAACCCATATATGAACAAGCCTCTGTTAAGACACTACCTGCT AACCCCTCAAGAGAACAACTTACAGAACTGGAGAAAAGGGACTTGCTAGATACCAATGATTATGAAGAA TATAAGGTACCTACTGATATGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_144722 |
| Insert Size | 1545 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144722.3 |
| RefSeq Size | 2982 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 79925 |
| UniProt ID | Q9C093 |
| MW | 61.2 kDa |
| Gene Summary | Required for correct axoneme development in spermatozoa. Important for normal development of the manchette and sperm head morphology. Essential for male fertility. Plays a role in localization of the intraflagellar transport protein IFT20 to the manchette, suggesting function as an adapter for dynein-mediated protein transport during spermatogenesis. Also plays a role in bone growth where it seems to be required for normal osteoblast differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks more than half of the 3'-most exons and has a 3' end that extends into an intron compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223626 | SPEF2 (Myc-DDK-tagged)-Human sperm flagellar 2 (SPEF2), transcript variant 2 |
CNY 3832.00 |
|
| RC223626L3 | Lenti ORF clone of Human sperm flagellar 2 (SPEF2), transcript variant 2, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC223626L4 | Lenti ORF clone of Human sperm flagellar 2 (SPEF2), transcript variant 2, mGFP tagged |
CNY 6460.00 |
|
| RG223626 | SPEF2 (tGFP-tagged) - Human sperm flagellar 2 (SPEF2), transcript variant 2 |
CNY 5040.00 |
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