ZCCHC6 (BC032456) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp666B142; DKFZp686C11112; DKFZp686F119; DKFZp686I1269; PAP associated domain containing 6; PAPD6; zinc finger, CCHC domain containing 6
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032456, the custom clone sequence may differ by one or more nucleotides ATGGGAGATACAGCAAAACCTTATTTCGTGAAGCGCACTAAAGACCGGGGGACTATGGAT GATGATGACTTCAGAAGGGGTCACCCCCAACAAGATTATTTAATAATAGATGACCATGCT AAAGGCCATGGCAGTAAAATGGAAAAGGGCCTTCAAAAAAAGAAGATAACACCAGGGAAC TATGGGAATACCCCCAGAAAAGGACCATGTGCTGTTTCCAGCAATCCATATGCATTTAAA AACCCAATCTACAGTCAACCCGCTTGGATGAATGACAGCCACAAAGATCAGAGTAAGAGA TGGCTGTCTGATGAACATACTGGTAATTCAGACAACTGGAGAGAATTCAAACCTGGACCT AGAATTCCTGTTATAAACCGACAAAGAAAAGACTCCTTTCAAGAAAATGAAGATGGTTAT AGGTGGCAAGACACAAGAGGCTGCAGAACTGTAAGACGACTGTTTCATAAAGACCTAACA AGCCTAGAAACCACGTCAGAAATGGAAGCAGGAAGTCCTGAAAACAAGAAGCAGAGGTCC AGACCTAGGAAGCCACGGAAGACTAGAAATGAGGAAAATGAGCAGGATGGAGACTTGGAA GGCCCTGTGATCGATGAGTCTGTACTTTCAACGAAGGAGCTGCTAGGCTTACAGCAGGCT GAGGAGAGACTGAAGAGAGACTGCATTGACAGGCTAAAAAGGCGACCACGAAACTACCCT ACAGCAAAGTACACCTGCAGACTCTGTGATGTTTTAATTGAATCCATTGCATTTGCCCAT AAGCATATCAAGGAAAAGAGGCACAAGAAAAACATTAAGGAGAAGCAAGAGGAAGAGTTG CTCACTACGTTACCCCCACCAACACCCTCCCAGATAAATGCAGTTGGCATTGCCATTGAC AAAGTGGTACAGGAATTTGGCTTACACAATGAGAACTTGGAACAGAGGCTGGAAATTAAA CGTATCATGGAAAATGTGTTCCAACACAAGTTACCAGATTGTTCCCTAAGATTATATGGG TCATCCTGTAGCAGATTGGGTTTCAAAAATTCGGATGTAAACATTGACATCCAGTTTCCA GCCATTATGTCTCAGCCAGATGTCCTCTTACTTGTTCAAGAATGTTTAAAGAACAGTGAC TCCTTTATTGATGTTGATGCAGACTTCCATGCTAGGGTGCCAGTGGTGGTGTGCAGAGAA AAGCAAAGGTCTCATTTCTTTAAACTGTTTATTTAC
Restriction Sites	Please inquire
ACCN	BC032456
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032456.1, AAH32456.1
RefSeq Size	1614 bp
RefSeq ORF	1614 bp
Locus ID	79670
Domains	ZnF_U1
Gene Summary	Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351).[UniProtKB/Swiss-Prot Function]

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