RUFY4 (NM_198483) Human Untagged Clone
CAT＃: SC313301
RUFY4 (untagged)-Human RUN and FYVE domain containing 4 (RUFY4), transcript variant 1
CNY 9220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ZFYVE31 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC313301 representing NM_198483.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGAAGAGGGAGCCATCCTCAAGGTCACCAAAGACCTAAGAGCTGCCGTCTCTGCCATCCTCCAG GGCTATGGGGATGGGCAGGGGCCAGTGACGGACACCAGTGCCGAGCTGCACAGACTCTGTGGCTGCCTG GAGCTGCTGCTGCAGTTTGACCAGAAAGAGCAGAAGAGCTTCCTGGGGCCTCGGAAGGATTACTGGGAC TTTCTCTGCACTGCCCTACGACGGCAGCGGGGAAACATGGAGCCAATCCACTTTGTCCGTTCCCAGGAC AAGTTGAAGACCCCTCTGGGGAAAGGCCGTGCCTTCATCCGCTTCTGCCTGGCCCGTGGGCAGCTGGCT GAGGCCCTGCAGCTTTGCCTCCTGAACTCAGAGCTCACCAGGGAATGGTATGGACCCCGGAGCCCTCTG CTCTGCCCAGAACGCCAAGAAGACATCCTGGACTCTCTCTATGCTCTCAATGGGGTGGCCTTCGAGTTG GACCTCCAGCAGCCAGACCTGGATGGAGCCTGGCCCATGTTCTCAGAGTCACGCTGCTCCAGTTCCACC CAAACCCAGGGAAGGAGACCCAGAAAAAACAAAGATGCCCCAAAGAAGATCCCAGCCGCATATGGAGGG CCTGAAAATGTCCAGATTGAGGACTCACACACCAGTCAAGCCATCTGTCTGCAAGATGCACCCAGTGGA CAGCAGCTGGCAGGGCTTCCCAGGTCCCAGCAACAAAGGCATCTTCCTTTCTTTTTGGAAAAGAAGGGG GAAAGTTCCAGGAAACATAGGTACCCCCAGAGCATGTGGGAGCCAGAAGGGAAGGAGCTTCAGCTAGAC CAGGAGGAAAGAGCCCCATGGATTGAGATCTTCCTGGGGAACTCAACACCCAGCACCCAGGGACAGGGG AAGGGGGCTATGGGCACTCAGAAGGAGGTGATAGGGATGGAGGCTGAGGTCACAGGGGTTCTGCTGGTT GCAGAGGGTCAGAGAACAACAGAGGGGACTCACAAAAAGGAAGCAGAGTGGAGTCACGTCCAGAGGCTG CTGATGCCCAGCCCCAGAGGGGCTGTAGAGGGAGCAGTATCAGGGAGCAGGCAGGGGTCGGGGGGCTCT AGCATCCTGGGGGAGCCCTGGGTCCTTCAGGGACACGCAACAAAGGAAGACTCTACCGTGGAGAATCCA CAAGTGCAAACAGAAGTGACCCTTGTGGCCAGAAGGGAGGAGCAAGCCGAGGTGTCCCTGCAGGACGAG ATCAAGAGCCTCAGACTTGGGCTCCGGAAGGCTGAGGAGCAGGCCCAGCGCCAGGAGCAGCTGCTGAGG GAGCAGGAGGGGGAGCTGCAGGCACTTCGGGAGCAGCTCAGCAGGTGTCAGGAAGAGAGAGCCGAGCTG CAGGCACAGCTGGAGCAGAAGCAACAGGAGGCTGAGAGGAGGGATGCCATGTACCAGGAGGAGCTTGGA GGGCAGCGGGACTTGGTCCAGGCCATGAAGAGGCGGGTGTTGGAACTGATCCAAGAGAAGGACCGCCTG TGGCAGAGGCTCCAGCATCTCTCTTCCATGGCTCCCGAGTGCTGTGTGGCCTGTAGCAAGATCTTTGGC CGATTTTCTCGGCGGTATCCATGCAGGCTCTGTGGAGGCCTGCTCTGCCATGCTTGCTCCATGGATTAC AAGAAGAGAGACCGCTGCTGCCCACCCTGCGCCCAGGGAAGAGAAGCCCAGGTCACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_198483 |
| Insert Size | 1716 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198483.3 |
| RefSeq Size | 2777 bp |
| RefSeq ORF | 1716 bp |
| Locus ID | 285180 |
| UniProt ID | Q6ZNE9 |
| MW | 64.3 kDa |
| Gene Summary | Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript but encodes the supported protein. The exon combination of this variant lacks full-length support in human; it is predicted based on alignments of partial human transcripts and homologous transcripts from mouse GeneID:435626. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216656 | RUFY4 (Myc-DDK-tagged)-Human RUN and FYVE domain containing 4 (RUFY4), transcript variant 1 |
CNY 3656.00 |
|
| RC216656L3 | Lenti ORF clone of Human RUN and FYVE domain containing 4 (RUFY4), transcript variant 1, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC216656L4 | Lenti ORF clone of Human RUN and FYVE domain containing 4 (RUFY4), transcript variant 1, mGFP tagged |
CNY 6460.00 |
|
| RG216656 | RUFY4 (tGFP-tagged) - Human RUN and FYVE domain containing 4 (RUFY4), transcript variant 1 |
CNY 5040.00 |
