GLEPP1 (PTPRO) (NM_030668) Human Untagged Clone

CAT#: SC313211

PTPRO (untagged)-Human protein tyrosine phosphatase, receptor type, O (PTPRO), transcript variant 4



  "NM_030668" in other vectors (4)

CNY 6,180.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PTPRO Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT; PTPU2; R-PTP-O
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC313211 representing NM_030668.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGTTACAGAGATGAATCCCAATGTGGTAGTGATCTCCGTGCTGGCCATCCTTAGCACACTTTTAATT
GGACTGTTGCTTGTTACCCTCATTATTCTTAGGAAAAAGCATCTGCAGATGGCTAGGGAGTGTGGAGCT
GGTACATTTGTCAATTTTGCATCCTTAGAGAGGGATGGAAAGCTTCCATACAACTGGAGTAAAAATGGT
TTAAAGAAGAGGAAACTGACAAACCCGGTTCAACTGGATGACTTTGATGCCTATATTAAGGATATGGCC
AAAGACTCTGACTATAAATTTTCTCTTCAGTTTGAGGAGTTGAAATTGATTGGACTGGATATCCCACAC
TTTGCTGCAGATCTTCCACTGAATCGATGTAAAAACCGTTACACAAACATCCTACCATATGACTTCAGC
CGTGTGAGATTAGTCTCCATGAATGAAGAGGAAGGTGCAGACTACATCAATGCCAACTATATTCCTGGA
TACAACTCACCCCAGGAGTATATTGCCACCCAGGGGCCACTGCCTGAAACCAGAAATGACTTCTGGAAG
ATGGTCCTGCAACAAAAGTCTCAGATTATTGTCATGCTCACTCAGTGTAATGAGAAAAGGAGGGTGAAA
TGTGACCATTACTGGCCATTCACGGAAGAACCTATAGCCTATGGAGACATCACTGTGGAGATGATTTCA
GAGGAAGAGCAGGACGACTGGGCCTGTAGACACTTCCGGATCAACTATGCTGACGAGATGCAGGATGTG
ATGCATTTTAACTACACTGCATGGCCTGATCATGGTGTGCCCACAGCAAATGCTGCAGAAAGTATCCTG
CAGTTTGTACACATGGTCCGACAGCAAGCTACCAAGAGCAAAGGTCCCATGATCATTCACTGCAGTGCT
GGCGTGGGACGGACAGGAACATTCATTGCCCTGGACAGGCTCTTGCAGCACATTCGGGATCATGAGTTT
GTTGACATCTTAGGGCTGGTGTCAGAAATGAGGTCATACCGGATGTCTATGGTACAGACAGAGGAGCAG
TACATTTTTATCCATCAGTGTGTGCAACTGATGTGGATGAAGAAGAAGCAGCAGTTCTGCATCAGTGAT
GTCATATACGAGAATGTTAGCAAGTCCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_030668
Insert Size 1134 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_030668.2
RefSeq Size 3886 bp
RefSeq ORF 1134 bp
Locus ID 5800
UniProt ID Q16827
Domains Y_phosphatase, PTPc_motif
Protein Families Phosphatase, Transmembrane
MW 43.8 kDa
Gene Summary This gene encodes a member of the R3 subtype family of receptor-type protein tyrosine phosphatases. These proteins are localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. This gene contains two distinct promoters, and alternatively spliced transcript variants encoding multiple isoforms have been observed. The encoded proteins may have multiple isoform-specific and tissue-specific functions, including the regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor, and decreased expression of this gene has been observed in several types of cancer. [provided by RefSeq, May 2011]
Transcript Variant: This variant (4) differs in the 5' UTR and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. Variants 4 and 6 encode the same isoform (d), which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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