UBE2V1 (TMEM189-UBE2V1) (NM_199203) Human Untagged Clone
CAT＃: SC313174
TMEM189 (untagged)-Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1)
CNY 6080.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC313174 representing NM_199203.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGGCGCCGAGGACTGGCCGGGCCAGCAGCTGGAGCTGGACGAGGACGAGGCGTCTTGTTGCCGC TGGGGCGCGCAGCACGCCGGGGCCCGCGAGCTGGCTGCGCTCTACTCGCCAGGCAAGCGCCTCCAGGAG TGGTGCTCTGTGATCCTGTGCTTCAGCCTCATCGCCCACAACCTGGTCCATCTCCTGCTGCTGGCCCGC TGGGAGGACACACCCCTCGTCATACTCGGTGTTGTTGCAGGGGCTCTCATTGCTGACTTCTTGTCTGGC CTGGTACACTGGGGTGCTGACACATGGGGCTCTGTGGAGCTGCCCATTGTGGGGAAGGCTTTCATCCGA CCCTTCCGGGAGCACCACATTGACCCGACAGCTATCACACGGCACGACTTCATCGAGACCAACGGGGAC AACTGCCTGGTGACACTGCTGCCGCTGCTAAACATGGCCTACAAGTTCCGCACCCACAGCCCTGAAGCC CTGGAGCAGCTATACCCCTGGGAGTGCTTCGTCTTCTGCCTGATCATCTTCGGCACCTTCACCAACCAG ATCCACAAGTGGTCGCACACGTACTTTGGGCTGCCACGCTGGGTCACCCTCCTGCAGGACTGGCATGTC ATCCTGCCACGTAAACACCATCGCATCCACCACGTCTCACCCCACGAGACCTACTTCTGCATCACCACA GGAGTAAAAGTCCCTCGCAATTTCCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAAGGAGTAGGAGAT GGCACAGTTAGCTGGGGTCTAGAAGATGACGAAGACATGACACTTACAAGATGGACAGGGATGATAATT GGGCCTCCAAGAACAATTTATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCA GAAGCACCCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGTAAATAGTTCTAATGGAGTG GTGGACCCAAGAGCCATATCAGTGCTAGCAAAATGGCAGAATTCATATAGCATCAAAGTTGTCCTGCAA GAGCTTCGGCGCCTAATGATGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACAGTGTTAC AGCAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_199203 |
| Insert Size | 1113 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199203.2 |
| RefSeq Size | 2925 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 387522 |
| UniProt ID | Q13404 |
| Protein Families | Transmembrane |
| MW | 42.2 kDa |
| Gene Summary | The TMEM189-UEV mRNA is an infrequent but naturally occurring read-through transcript of the neighboring TMEM189 and UBE2V1 genes. Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein produced by this transcript has UEV1 B domains but the protein is localized to the cytoplasm rather than to the nucleus. The significance of this read-through mRNA and the function of its protein product has not yet been determined. [provided by RefSeq, Oct 2010] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223133 | TMEM189 (Myc-DDK-tagged)-Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1) |
CNY 3656.00 |
|
| RC223133L3 | Lenti ORF clone of Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC223133L4 | Lenti ORF clone of Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), mGFP tagged |
CNY 5890.00 |
|
| RG223133 | TMEM189 (tGFP-tagged) - Human TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1) |
CNY 4370.00 |
