LINS (LINS1) (NM_001040616) Human Untagged Clone
CAT＃: SC311144
LINS (untagged)-Human lines homolog (Drosophila) (LINS), transcript variant 6
CNY 14540.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LINS; MRT27; WINS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC311144 representing NM_001040616.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAGTTTTCTGTGAAGTTTTAGAAGAGTTATACAAGAAGGTACTTCTTGGAGCCACACTTGAAAAT GACAGCCATGATTACATCTTTTATCTCAACCCAGCAGTTTCAGATCAAGATTGTTCTACAGCCACCTCC TTAGAATGGGCAAACACCTGTGGTATCCAGGGCAGGCATCAGCCCATCTCTGTTGGTGTGGCTCCCATT GCTGTAGCACCTGTGTGTTTGAAGACCAACTCTCAGATGAGCGGTTCCAGAGAAGTAATGCTCCTTCAG TTAACAGTGATCAAAGTGATGACAACCCGGATATTGTCTGTCAAAACCGAGTTCCATGCAAAGGAGCAG TACAGAGATGTAATTAAAATTCTCTTAGAATCAGCCAAAGTCGATTCTAAATTAATCTGCATGTTCCAA AATTCAGATAAATTGTTATCTCACATGGCTGCACAGTGCCTTGCATTGCTTCTATATTTCCAATTGAGA GAAAAGATAACCTTAAGTAATTCCTGGATTGCTTTTTGCCAGAAAAATCTTTCTGAATACTCTGAGAGT AATAAAGCAATATACTGCCTCTGGACTCTTACAGCAATAATAAAAGAAATCTTTAAAGATTCATGTTCA CAGAAAACAGAAATTCTAAAGCAGTTCCTGACTCATTTCGACACCATTTTTGAAGTGTTTTACAATTCC TTATTCTCTCAGCATTTTGAAAACTGCCGGGATACTTCTAAAATAGTAAACATCCTGATGTGTTTCCTG GATTTGCTTGAGCTTCTCATCGCCTCCAGAATCCACCTGAAGTTACATTTCACTTGCCAGAGGATTTTA TTTTTGAAACCATCTTGCATGCTAGAAGTTATTACCTGGCCTATTCAGGCTTTTGTTAAAAGGAAGGTC ATCATATTCCTCAAAAAGTGCCTTCTCTGTAAAGTGGGTGAAGACCTCTGTCGTGGATCTGTGCCTGCC TTAATGCCGCCAGACCATCATGTAGCGGTGGACATGCTGGCTTTAGCTAATGCTGTTTTGCAAGCTGTG AATTCGGGGTTGTTGAAGACACTGTCTGTTTATGAAAAACATTCCTTTTTTGGAGGTGATGAAGTTCAA CCTGAATGTGAACTTATCACTAGTCCAGATCATGTGATCCTTAGAGCAGCAAGCTTAGTTATAATGAAA TCCTTAGAAATCAAGTTTCAAAATTATTCTTCAGCAAGTGAAATGAAAGTTGACTTACAGAGGTTCATG TCTGAGTTACTGACCTTCTTAAAGCCTCATCTTCAGCCCTCTCTGCAATTACACAATCCGTGCAAATGG CTGTCTCGGGTTTTCATAGAACAAGACGATGACATGCTGGAGGCTGCCAAGGCATCACTGGGCATCTAC TTAACACTGACCAGAGGATGTGAAGCCACTGAAAGCTTGACTCAGGGAAAAGAAATGTGGGACCATCAC ACACATGAAAATGGCTATAATCCTCACTGTATTTTCTTGTTCTTCTTGAAAAATATAGGATTTGATTCC ACAGTTCTTCTTGACTTTTTGATTTCATCAGAAACCTGTTTTCTTGAATATTTTGTTAGATATTTAAAA TTACTGCAAAAGGACTGGGATAATTTTTTCACCATTTGCAATAACTTTGATGCAACTGAATCTAAATAT GACATAAGTATTTGTGGCTGTGTCCCCTCACTTGTCCAAGACCAAAGCTCCAACCAAACAATACCCCAT CGTTTGACTGCTCCTCATAGTCACAGAGATGTGTGTGCTCGGCACTCCTGGGCTTCCGATGCTCCCTCT GAACCACTGAAAGCTGTGATGTCCAAGGGGGCTCACACCATGTGTGCTTCTAGTCTGTCTTCCCCCCGG GCCTCTCAAAGTCTGGTAGATTACGACAGCTCTGACGATTCTGACGTGGAATCCACAGAGCAGTGTTTA GCTAACAGTAAACAGACATCTTTACACCAGCAAGCAACTAAGGAGATTCAGGATGCAGCTGGGACAAGC AGGGATAAAAAAGAATTTAGCCTTGAGCCTCCATCAAGGCCTCTGGTTCTGAAAGAATTTGATACTGCC TTCTCCTTTGATTGTGAAGTAGCCCCAAATGATGTCGTCTCTGAAGTGGGAATATTTTACAGAATAGTA AAATGCTTCCAGGAACTACAAGATGCCATCTGCCGTTTGCAAAAGAAAAATCTTTTCCCATATAATCCA ACTGCACTTTTGAAGTTGTTAAAATACATAGAGGTGATAAGTAATAAAACTATGAACACTTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040616 |
| Insert Size | 2274 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040616.2 |
| RefSeq Size | 2512 bp |
| RefSeq ORF | 2274 bp |
| Locus ID | 55180 |
| UniProt ID | Q8NG48 |
| MW | 85.9 kDa |
| Gene Summary | The Drosophila segment polarity gene lin encodes a protein, lines, which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215586 | LINS (Myc-DDK-tagged)-Human lines homolog (Drosophila) (LINS), transcript variant 6 |
CNY 8312.00 |
|
| RC215586L1 | Lenti-ORF clone of LINS (Myc-DDK-tagged)-Human lines homolog (Drosophila) (LINS), transcript variant 6 |
CNY 10712.00 |
|
| RC215586L2 | Lenti-ORF clone of LINS (mGFP-tagged)-Human lines homolog (Drosophila) (LINS), transcript variant 6 |
CNY 7700.00 |
|
| RC215586L3 | Lenti-ORF clone of LINS (Myc-DDK-tagged)-Human lines homolog (Drosophila) (LINS), transcript variant 6 |
CNY 7700.00 |
|
| RC215586L4 | Lenti-ORF clone of LINS (mGFP-tagged)-Human lines homolog (Drosophila) (LINS), transcript variant 6 |
CNY 7700.00 |
|
| RG215586 | LINS (tGFP-tagged) - Human lines homolog (Drosophila) (LINS), transcript variant 6 |
CNY 6370.00 |
