CYB561A3 (NM_153611) Human Untagged Clone

CAT#: SC310606

CYBASC3 (untagged)-Human cytochrome b, ascorbate dependent 3 (CYBASC3), transcript variant 2



  "NM_153611" in other vectors (4)

CNY 3990.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CYBASC3; LCYTB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310606 representing NM_153611.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGTGTCTGGACGGTTCTACTTGTCCTGCCTGCTGCTGGGGTCCCTGGGCTCTATGTGCATCCTCTTC
ACTATCTACTGGATGCAGTACTGGCGTGGTGGCTTTGCCTGGAATGGCAGCATCTACATGTTCAACTGG
CACCCAGTGCTTATGGTTGCTGGCATGGTGGTATTCTATGGAGGTGCGTCACTGGTGTACCGCCTGCCC
CAGTCGTGGGTGGGGCCCAAACTGCCCTGGAAACTCCTCCATGCAGCGCTGCACCTGATGGCCTTCGTC
CTCACTGTTGTGGGGCTGGTTGCTGTCTTTACGTTTCACAACCATGGAAGGACTGCCAACCTCTACTCC
CTTCACAGCTGGCTGGGCATCACCACTGTCTTCCTCTTCGCCTGCCAGTGGTTCCTGGGCTTTGCTGTC
TTCCTCCTGCCCTGGGCGTCCATGTGGCTGCGCAGCCTCCTAAAACCTATCCACGTCTTTTTTGGAGCC
GCCATCCTCTCTCTGTCCATCGCATCCGTCATTTCGGGCATTAATGAGAAGCTTTTCTTCAGTTTGAAA
AACACCACCAGGCCATACCACAGCCTGCCCAGTGAGGCGGTCTTTGCCAACAGCACCGGGATGCTGGTG
GTGGCCTTTGGGCTGCTGGTGCTCTACATCCTTCTGGCTTCATCTTGGAAGCGCCCAGAGCCGGGGATC
CTGACCGACAGACAGCCCCTGCTGCATGATGGGGAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_153611
Insert Size 729 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153611.4
RefSeq Size 3045 bp
RefSeq ORF 729 bp
Locus ID 220002
UniProt ID Q8NBI2
Domains Cytochrome_B561
Protein Families Transmembrane
MW 27.2 kDa
Gene Summary Ferric-chelate reductase that reduces Fe(3+) to Fe(2+) before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of the last exon compared to variant 4. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 3. Variants 2 and 3 both encode the same isoform (2).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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