BRME1 (NM_024323) Human Untagged Clone
CAT＃: SC310205
C19orf57 (untagged)-Human chromosome 19 open reading frame 57 (C19orf57)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C19orf57; MEIOK21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310205 representing NM_024323.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTAAGAGGAAGAAGCTGCGGACCTCAGGAGAGGGACTCTGTCCTCCAAAACCCCTAAAGAACCCA AGGCTAGGAGACTTCTATGGGGACCCCCAGAGTTCCATGTTGGGCTGTTTACATCACCCTGAGGAGCCA GAGGGCAAATTGGGACCTGTTCCCTCTACACAGCAGCACGGGGAGGAACCAGGAAAGGCCGTCTCCAGC TCCCCTGATGAGGAAACAGGATCTCCCTGCCGGCTCCTCCGTCAACCAGAAAAGGAGCCAGCTCCCCTT CCTCCTTCCCAGAACTCATTCGGGAGGTTTGTTCCCCAGTTTGCAAAATCCAGGAAGACAGTGACAAGA AAAGAAGAGATGAAGGATGAGGACCGTGGGAGTGGGGCCTTTAGCCTGGAAACAATCGCAGAGTCCAGC GCCCAGAGTCCAGGATGCCAGCTGCTAGTGGAGACCCTGGGGGTCCCCCTCCAGGAGGCCACGGAGCTG GGGGACCCAACGCAGGCAGACAGTGCCCGCCCTGAGCAGAGCAGCCAGAGCCCTGTGCAGGCGGTGCCC GGCAGTGGGGATTCTCAGCCTGATGACCCTCCAGACAGGGGGACGGGGTTGTCCGCCTCACAGAGGGCC AGCCAAGACCACCTGTCAGAACAAGGGGCCGATGACAGCAAGCCTGAGACAGACAGGGTTCCAGGTGAC GGTGGCCAAAAGGAACACCTACCAAGCATTGATTCTGAAGGGGAGAAGCCAGACAGAGGAGCCCCCCAG GAGGGAGGGGCCCAAAGGACAGCAGGGGCTGGCCTGCCTGGAGGGCCCCAGGAGGAGGGAGACGGTGTC CCCTGTACCCCAGCATCAGCTCCTACCTCAGGCCCTGCTCCAGGACTGGGCCCTGCCTCTTGGTGCCTG GAACCCGGGTCTGTGGCCCAGGGCTCCCCTGACCCCCAGCAGACCCCCAGCAGGATGGGTAGGGAAGGG GAAGGGACTCATAGCAGCCTGGGATGCTCCTCCCTCGGGATGGTTGTCATCGCAGACCTGAGCACAGAC CCCACTGAGCTGGAAGAGAGGGCTCTGGAGGTGGCTGGGCCCGATGGGCAGGCCAGTGCCATATCACCT GCCTCTCCCAGGAGGAAGGCCGCTGATGGAGGCCACAGGAGGGCCTTGCCAGGCTGCACCTCGCTCACT GGGGAAACCACAGGAGAAAGTGGGGAGGCAGGGCAGGATGGCAAGCCCCCCGGCGATGTCCTAGTGGGC CCTACAGCCTCCCTGGCTCTGGCACCTGGGAGCGGAGAGTCCATGATGGGTGCTGGAGATTCCGGTCAT GCATCCCCGGACACAGGTCCATGTGTCAATCAGAAGCAGGAGCCAGGTCCTGCTCAAGAGGAAGCCGAG TTAGGTGGCCAGAACCTCGAACGAGACCTCGAGGGGTTCCGTGTGTCCCCGCAAGCCTCTGTTGTGCTG GAACACAGAGAAATAGCAGACGACCCTCTCCAGGAGCCCGGGGCTCAGCAGGGCATTCCAGACACCACC TCAGAGCTGGCAGGGCAGCGAGACCACCTGCCTCATTCTGCAGACCAGGGCACCTGGGCAGACTCTTTA GCTGTGGAACTCGACTTCCTGCTGGACAGCCAGATACAGGATGCCCTGGACGCCTCTGACTTCGAAGCC CCACCTGAGCAGCTCTTTCCTTCGGGGAACAAGCCGGGCCCTTGCTGGCCGGGCCCCAGCTCACATGCC AATGGAGACCCTGTTGCAGTGGCCAAGGCCCAGCCGAGCCGGCTGATCATGGGCACCCACCGGGACCTG GAAGCTTTCAAGCGCCTTAACTACCGGAAGACAAAGCTGGGAGGCAAAGCCCCCCTGCCTTACCCTTCC AAGGGGCCTGGGAATATCCCTCGAGGGGACCCACCCTGGAGGGAGTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024323 |
| Insert Size | 1914 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024323.3 |
| RefSeq Size | 2420 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 79173 |
| UniProt ID | Q0VDD7 |
| MW | 66.2 kDa |
| Gene Summary | Meiotic recombination factor component of recombination bridges involved in meiotic double-strand break repair. Modulates the localization of recombinases DMC1:RAD51 to meiotic double-strand break (DSB) sites through the interaction with and stabilization of the BRCA2:HSF2BP complex during meiotic recombination. Indispensable for the DSB repair, homologous synapsis, and crossover formation that are needed for progression past metaphase I, is essential for spermatogenesis and male fertility.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an alternate segment in the C-terminus compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212766 | C19orf57 (Myc-DDK-tagged)-Human chromosome 19 open reading frame 57 (C19orf57) |
CNY 4744.00 |
|
| RC212766L3 | Lenti ORF clone of Human chromosome 19 open reading frame 57 (C19orf57), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC212766L4 | Lenti ORF clone of Human chromosome 19 open reading frame 57 (C19orf57), mGFP tagged |
CNY 5890.00 |
|
| RG212766 | C19orf57 (tGFP-tagged) - Human chromosome 19 open reading frame 57 (C19orf57) |
CNY 6344.00 |
|
| SC318089 | C19orf57 (untagged)-Human chromosome 19 open reading frame 57 (C19orf57) |
CNY 7220.00 |
