HEC1 (NDC80) (NM_006101) Human Untagged Clone
CAT＃: SC310202
NDC80 (untagged)-Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310202 representing NM_006101.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGCGCAGTTCAGTTTCCAGCGGTGGTGCTGGCCGCCTCTCCATGCAGGAGTTAAGATCCCAGGAT GTAAATAAACAAGGCCTCTATACCCCTCAAACCAAAGAGAAACCAACCTTTGGAAAGTTGAGTATAAAC AAACCGACATCTGAAAGAAAAGTCTCGCTATTTGGCAAAAGAACTAGTGGACATGGATCCCGGAATAGT CAACTTGGTATATTTTCCAGTTCTGAGAAAATCAAGGACCCGAGACCACTTAATGACAAAGCATTCATT CAGCAGTGTATTCGACAACTCTGTGAGTTTCTTACAGAAAATGGTTATGCACATAATGTGTCCATGAAA TCTCTACAAGCTCCCTCTGTTAAAGACTTCCTGAAGATCTTCACATTTCTTTATGGCTTCCTGTGCCCC TCATACGAACTTCCTGACACAAAGTTTGAAGAAGAGGTTCCAAGAATCTTTAAAGACCTTGGGTATCCT TTTGCACTATCCAAAAGCTCCATGTACACAGTGGGGGCTCCTCATACATGGCCTCACATTGTGGCAGCC TTAGTTTGGCTAATAGACTGCATCAAGATACATACTGCCATGAAAGAAAGCTCACCTTTATTTGATGAT GGGCAGCCTTGGGGAGAAGAAACTGAAGATGGAATTATGCATAATAAGTTGTTTTTGGACTACACCATA AAATGCTATGAGAGTTTTATGAGTGGTGCCGACAGCTTTGATGAGATGAATGCAGAGCTGCAGTCAAAA CTGAAGGATTTATTTAATGTGGATGCTTTTAAGCTGGAATCATTAGAAGCAAAAAACAGAGCATTGAAT GAACAGATTGCAAGATTGGAACAAGAAAGAGAAAAAGAACCGAATCGTCTAGAGTCGTTGAGAAAACTG AAGGCTTCCTTACAAGGAGATGTTCAAAAGTATCAGGCATACATGAGCAATTTGGAGTCTCATTCAGCC ATTCTTGACCAGAAATTAAATGGTCTCAATGAGGAAATTGCTAGAGTAGAACTAGAATGTGAAACAATA AAACAGGAGAACACTCGACTACAGAATATCATTGACAACCAGAAGTACTCAGTTGCAGACATTGAGCGA ATAAATCATGAAAGAAATGAATTGCAGCAGACTATTAATAAATTAACCAAGGACCTGGAAGCTGAACAA CAGAAGTTGTGGAATGAGGAGTTAAAATATGCCAGAGGCAAAGAAGCGATTGAAACACAATTAGCAGAG TATCACAAATTGGCTAGAAAATTAAAACTTATTCCTAAAGGTGCTGAGAATTCCAAAGGTTATGACTTT GAAATTAAGTTTAATCCCGAGGCTGGTGCCAACTGCCTTGTCAAATACAGGGCTCAAGTTTATGTACCT CTTAAGGAACTCCTGAATGAAACTGAAGAAGAAATTAATAAAGCCCTAAATAAAAAAATGGGTTTGGAG GATACTTTAGAACAATTGAATGCAATGATAACAGAAAGCAAGAGAAGTGTGAGAACTCTGAAAGAAGAA GTTCAAAAGCTGGATGATCTTTACCAACAAAAAATTAAGGAAGCAGAGGAAGAGGATGAAAAATGTGCC AGTGAGCTTGAGTCCTTGGAGAAACACAAGCACCTGCTAGAAAGTACTGTTAACCAGGGGCTCAGTGAA GCTATGAATGAATTAGATGCTGTTCAGCGGGAATACCAACTAGTTGTGCAAACCACGACTGAAGAAAGA CGAAAAGTGGGAAATAACTTGCAACGTCTGTTAGAGATGGTTGCTACACATGTTGGGTCTGTAGAGAAA CATCTTGAGGAGCAGATTGCTAAAGTTGATAGAGAATATGAAGAATGCATGTCAGAAGATCTCTCGGAA AATATTAAAGAGATTAGAGATAAGTATGAGAAGAAAGCTACTCTAATTAAGTCTTCTGAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_006101 |
| Insert Size | 1929 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006101.2 |
| RefSeq Size | 2209 bp |
| RefSeq ORF | 1929 bp |
| Locus ID | 10403 |
| UniProt ID | O14777 |
| Domains | Ndc80_HEC |
| Protein Families | Druggable Genome |
| MW | 73.9 kDa |
| Gene Summary | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207565 | NDC80 (Myc-DDK-tagged)-Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80) |
CNY 4784.00 |
|
| RC207565L1 | Lenti ORF clone of Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80), Myc-DDK-tagged |
CNY 7184.00 |
|
| RC207565L2 | Lenti ORF clone of Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80), mGFP tagged |
CNY 5890.00 |
|
| RC207565L3 | Lenti ORF clone of Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC207565L4 | Lenti ORF clone of Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80), mGFP tagged |
CNY 5890.00 |
|
| RG207565 | NDC80 (tGFP-tagged) - Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80) |
CNY 6384.00 |
