WDR35 (NM_020779) Human Untagged Clone
CAT＃: SC309962
WDR35 (untagged)-Human WD repeat domain 35 (WDR35), transcript variant 2
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CED2; FAP118; IFT121; IFTA1; SRTD7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309962 representing NM_020779.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCTTCTACCTGAGCAAGAAAATTTCCATTCCCAATAACGTGAAGCTGCAGTGTGTATCCTGGAAC AAGGAACAAGGGTTCATAGCATGCGGTGGTGAAGATGGATTACTGAAAGTTTTGAAATTAGAGACGCAG ACAGATGATGCAAAATTGAGGGGCCTTGCAGCCCCCAGTAACCTTTCTATGAATCAGACTCTTGAAGGT CATAGTGGTTCTGTTCAAGTTGTAACATGGAATGAGCAGTATCAGAAGTTGACTACCAGTGATGAAAAC GGGCTTATCATTGTGTGGATGTTATATAAAGGCTCTTGGATTGAGGAGATGATCAACAATCGAAATAAA TCAGTTGTTCGCAGTATGAGCTGGAATGCTGACGGACAGAAGATCTGCATTGTATATGAAGATGGGGCT GTGATAGTTGGTTCAGTGGATGGCAATCGTATTTGGGGAAAAGACCTGAAGGGTATACAGCTATCCCAT GTAACATGGTCTGCGGACAGTAAAGTCTTACTTTTTGGAATGGCAAATGGGGAAATACACATTTACGAT AATCAAGGAAATTTTATGATAAAAATGAAACTGAGTTGTTTGGTGAATGTCACTGGAGCTATCAGCATT GCTGGAATTCATTGGTACCATGGCACAGAAGGCTACGTGGAGCCTGATTGCCCTTGCCTTGCTGTTTGC TTTGATAATGGAAGATGCCAAATAATGAGACATGAGAATGACCAAAATCCCGTTTTGATTGACACTGGC ATGTACGTAGTAGGCATCCAGTGGAACCACATGGGCAGCGTGTTAGCTGTGGCAGGCTTCCAGAAGGCA GCCATGCAGGACAAAGATGTGAACATTGTGCAGTTTTACACTCCGTTTGGTGAGCATCTGGGTACTTTG AAAGTTCCTGGAAAGGAAATATCTGCACTATCTTGGGAAGGAGGTGGACTGAAAATTGCACTAGCTGTT GATTCCTTTATATATTTTGCAAACATTCGACCTAATTATAAGTGGGGTTATTGCTCAAACACTGTAGTT TATGCATATACCAGACCTGATCGTCCAGAATATTGTGTTGTCTTCTGGGATACGAAAAACAATGAAAAA TATGTTAAATATGTGAAGGGTCTCATTTCTATTACTACCTGTGGAGATTTCTGCATTTTGGCTACAAAA GCTGATGAAAATCATCCTCAGTTTGTACTAGTTCTTTGTAATTCTATTGGTACACCCTTGGATCCCAAA TACATTGATATTGTACCATTGTTTGTTGCAATGACCAAAACCCATGTGATAGCAGCCTCGAAAGAAGCA TTTTATACCTGGCAATATCGTGTGGCAAAGAAGCTCACAGCATTGGAAATTAATCAGATCACACGGTCT CGAAAAGAAGGGAGAGAAAGAATTTATCATGTTGATGATACCCCTTCTGGATCAATGGATGGTGTGCTT GATTATAGTAAAACCATTCAAGGCACAAGGGATCCAATTTGTGCCATAACTGCATCAGATAAGATATTG ATTGTGGGTCGTGAATCTGGCACCATTCAGAGATACAGTCTACCTAATGTTGGTTTGATTCAAAAATAT TCCCTTAATTGTCGAGCCTACCAGTTATCCTTGAATTGCAACTCTAGCCGTCTTGCTATCATAGACATC TCAGGAGTTCTGACTTTCTTTGACTTGGATGCTCGAGTAACGGACAGTACGGGACAGCAAGTAGTTGGA GAGTTGTTAAAATTGGAACGAAGAGATGTCTGGGATATGAAGTGGGCCAAAGATAATCCTGATTTGTTT GCAATGATGGAGAAGACAAGAATGTATGTTTTCAGAAACTTGGATCCTGAGGAACCCATTCAGACCTCT GGATATATTTGTAATTTTGAGGATTTAGAAATTAAATCTGTTCTTTTGGATGAGATATTAAAGGATCCA GAACATCCAAACAAGGATTACCTAATTAACTTTGAGATTCGGTCTCTGCGAGATAGCCGAGCACTGATT GAGAAGGTTGGAATTAAAGATGCATCTCAGTTCATAGAGGACAATCCACACCCCCGACTTTGGCGCCTA CTGGCTGAAGCAGCTCTTCAGAAACTGGATCTATACACTGCAGAGCAAGCATTTGTGCGCTGCAAAGAT TACCAAGGCATTAAGTTTGTGAAGCGCTTGGGCAAACTACTGAGTGAGTCAATGAAACAGGCTGAAGTT GTTGGCTACTTCGGCAGGTTTGAAGAGGCTGAAAGAACGTATCTCGAGATGGACAGAAGGGATCTTGCT ATTGGCCTCCGGCTGAAATTGGGGGATTGGTTTAGAGTACTCCAGCTCCTGAAAACTGGATCTGGTGAT GCAGATGACAGTCTCCTGGAACAAGCCAACAATGCCATTGGAGACTACTTTGCTGATCGACAAAAGTGG TTGAATGCTGTACAATATTATGTACAAGGACGGAACCAGGAACGCTTAGCTGAATGTTACTATATGTTA GAGGATTATGAAGGGTTAGAGAACCTTGCCATTTCACTTCCAGAAAACCACAAGTTACTTCCAGAAATA GCACAAATGTTTGTCAGAGTTGGAATGTGTGAACAAGCAGTGACTGCATTTTTGAAATGTAGTCAACCA AAGGCAGCAGTAGATACCTGCGTACATCTCAACCAATGGAACAAAGCTGTTGAATTGGCTAAAAATCAT AGTATGAAAGAAATTGGATCTCTGTTAGCTAGGTATGCATCTCATTTACTGGAAAAGAATAAAACTCTT GATGCCATAGAACTCTATCGGAAAGCCAATTACTTTTTTGATGCAGCTAAACTGATGTTTAAGATTGCA GATGAAGAGGCAAAGAAAGGAAGTAAACCTTTACGTGTCAAGAAGCTCTATGTACTGTCAGCCTTACTT ATAGAGCAATACCATGAACAGATGAAGAATGCCCAGCGAGGAAAAGTTAAAGGAAAAAGTTCAGAGGCC ACTTCTGCCTTGGCTGGTTTGCTGGAAGAAGAAGTTCTGTCTACAACAGATCGTTTCACAGATAATGCA TGGAGAGGGGCAGAGGCTTACCACTTCTTTATACTTGCACAGAGGCAGCTCTATGAGGGATGTGTGGAC ACTGCACTGAAGACAGCTCTTCACCTGAAAGACTATGAAGACATCATCCCTCCTGTGGAGATCTACTCT CTGCTAGCACTCTGCGCATGCGCCAGCAGAGCCTTTGGGACTTGTTCAAAAGCTTTCATTAAACTTAAA TCTTTAGAGACCCTCAGTTCAGAACAGAAACAGCAGTATGAAGACCTTGCTTTAGAAATCTTCACCAAA CATACTTCAAAAGATAACAGAAAACCTGAATTGGACAGCCTTATGGAAGGTGGAGAAGGGAAACTGCCA ACATGCGTTGCCACAGGAAGCCCAATCACTGAGTATCAATTCTGGATGTGCAGTGTATGCAAACACGGT GTCCTTGCTCAGGAAATAAGCCACTACAGCTTCTGCCCCTTATGCCATAGTCCAGTGGGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020779 |
| Insert Size | 3513 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020779.3 |
| RefSeq Size | 6924 bp |
| RefSeq ORF | 3513 bp |
| Locus ID | 57539 |
| UniProt ID | Q9P2L0 |
| MW | 132.3 kDa |
| Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Two patients with Sensenbrenner syndrome / cranioectodermal dysplasia (CED) were identified with mutations in this gene, consistent with a possible ciliary function.[provided by RefSeq, Sep 2010] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform 2 thus lacks an internal segment, as compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206081 | WDR35 (Myc-DDK-tagged)-Human WD repeat domain 35 (WDR35), transcript variant 2 |
CNY 8144.00 |
|
| RC206081L3 | Lenti ORF clone of Human WD repeat domain 35 (WDR35), transcript variant 2, Myc-DDK-tagged |
CNY 11880.00 |
|
| RC206081L4 | Lenti ORF clone of Human WD repeat domain 35 (WDR35), transcript variant 2, mGFP tagged |
CNY 11880.00 |
|
| RG206081 | WDR35 (tGFP-tagged) - Human WD repeat domain 35 (WDR35), transcript variant 2 |
CNY 11020.00 |
