Tankyrase (TNKS) (NM_003747) Human Untagged Clone
CAT＃: SC309919
TNKS (untagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS)
CNY 13872.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARTD5; PARP-5a; PARP5A; PARPL; pART5; TIN1; TINF1; TNKS1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC309919 sequence for NM_003747 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCGCGTCGCTCTCAGCATCATCACCACCATCATCAACAACAGCTCCAGCCCGCCCCAGGGG CTTCAGCGCCGCCGCCGCCACCTCCTCCCCCACTCAGCCCTGGCCTGGCCCCGGGGACCACCCCAGCCTC TCCCACGGCCAGCGGCCTGGCCCCCTTCGCCTCCCCGCGGCACGGCCTAGCGCTGCCGGAGGGGGATGGC AGTCGGGATCCGCCCGACAGGCCCCGATCCCCGGACCAGGTTGACGGTACCAGCTGTTGCAGTACCACCA GCACAATCTGTACCGTCGCCGCCGCTCCCGTGGTCCCAGCGGTTTCTACTTCATCTGCCGCTGGGGTCGC TCCCAACCCAGCCGGCAGTGGCAGTAACAATTCACCGTCGTCCTCTTCTTCCCCGACTTCTTCCTCATCT TCCTCTCCATCCTCCCCTGGATCGAGCTTGGCGGAGAGCCCCGAGGCGGCCGGAGTTAGCAGCACAGCAC CACTGGGGCCTGGGGCAGCAGGACCTGGGACTGGGGTCCCAGCAGTGAGCGGGGCCCTACGGGAACTGCT GGAGGCCTGTCGCAATGGGGACGTGTCCCGGGTAAAGAGACTGGTGGACGCGGCAAACGTAAATGCAAAG GACATGGCCGGCCGGAAGTCTTCTCCCCTGCACTTCGCTGCAGGTTTTGGAAGGAAGGATGTTGTAGAAC ACTTACTACAGATGGGTGCTAATGTCCACGCTCGTGATGATGGAGGTCTCATCCCGCTTCATAATGCCTG TTCTTTTGGCCATGCTGAGGTTGTGAGTCTGTTATTGTGCCAAGGAGCTGATCCAAATGCCAGGGATAAC TGGAACTATACACCTCTGCATGAAGCTGCTATTAAAGGGAAGATCGATGTGTGCATTGTGCTGCTGCAGC ACGGAGCTGACCCAAACATTCGGAACACTGATGGGAAATCAGCCCTGGACCTGGCAGATCCTTCAGCAAA AGCTGTCCTTACAGGTGAATACAAGAAAGACGAACTCCTAGAAGCTGCTAGGAGTGGTAATGAAGAAAAA CTAATGGCTTTACTGACTCCTCTAAATGTGAATTGCCATGCAAGTGATGGGCGAAAGTCGACTCCTTTAC ATCTAGCAGCGGGCTACAACAGAGTTCGAATAGTTCAGCTTCTTCTTCAGCATGGTGCTGATGTTCATGC AAAAGACAAAGGTGGACTTGTGCCTCTTCATAATGCATGTTCATATGGACATTATGAAGTCACAGAACTG CTACTAAAGCATGGAGCTTGTGTTAATGCCATGGATCTCTGGCAGTTTACTCCACTGCACGAGGCTGCTT CCAAGAACCGTGTAGAAGTCTGCTCTTTGTTACTTAGCCATGGCGCTGATCCTACGTTAGTCAACTGCCA TGGCAAAAGTGCTGTGGATATGGCTCCAACTCCGGAGCTTAGGGAGAGATTGACTTATGAATTTAAAGGT CATTCTTTACTACAAGCAGCCAGAGAAGCAGACTTAGCTAAAGTTAAAAAAACACTCGCTCTGGAAATCA TTAATTTCAAACAACCGCAGTCTCATGAAACAGCACTGCACTGTGCTGTGGCCTCTCTGCATCCCAAACG TAAACAAGTGACAGAATTGTTACTTAGAAAAGGAGCAAATGTTAATGAAAAAAATAAAGATTTCATGACT CCCCTACATGTTGCAGCCGAAAGAGCCCATAATGATGTCATGGAAGTTCTGCATAAGCATGGCGCCAAGA TGAATGCACTGGACACCCTTGGTCAGACTGCTTTGCATAGAGCCGCCCTAGCAGGCCACCTGCAGACCTG CCGCCTCCTGCTGAGTTACGGCTCTGACCCCTCCATCATCTCCTTACAAGGCTTCACAGCAGCACAGATG GGCAATGAAGCAGTGCAGCAGATTCTGAGTGAGAGTACACCTATACGTACTTCTGATGTTGATTATCGAC TCTTAGAGGCATCTAAAGCTGGAGACTTGGAAACTGTGAAGCAACTTTGCAGCTCTCAAAATGTGAATTG TAGAGACTTAGAGGGCCGGCATTCCACGCCCTTACACTTCGCAGCAGGCTACAACCGCGTGTCTGTTGTA GAGTACCTGCTACACCACGGTGCCGATGTCCATGCCAAAGACAAGGGTGGCTTGGTGCCCCTTCATAATG CCTGTTCATATGGACACTATGAGGTGGCTGAGCTTTTAGTAAGGCATGGGGCTTCTGTCAATGTGGCGGA CTTATGGAAATTTACCCCTCTCCATGAAGCAGCAGCTAAAGGGAAGTATGAAATCTGCAAGCTCCTTTTA AAACATGGAGCAGATCCAACTAAAAAGAACAGAGATGGAAATACACCTTTGGATTTGGTAAAGGAAGGAG ACACAGATATTCAGGACTTACTGAGAGGGGATGCTGCTTTGTTGGATGCTGCCAAGAAGGGCTGCCTGGC AAGAGTGCAGAAGCTCTGTACCCCAGAGAATATCAACTGCAGAGACACCCAGGGCAGAAATTCAACCCCT CTGCACCTGGCAGCAGGCTATAATAACCTGGAAGTAGCTGAATATCTTCTAGAGCATGGAGCTGATGTTA ATGCCCAGGACAAGGGTGGTTTAATTCCTCTTCATAATGCGGCATCTTATGGGCATGTTGACATAGCGGC TTTATTGATAAAATACAACACGTGTGTAAATGCAACAGATAAGTGGGCGTTTACTCCCCTCCATGAAGCA GCCCAGAAAGGAAGGACGCAGCTGTGCGCCCTCCTCCTAGCGCATGGTGCAGACCCCACCATGAAGAACC AGGAAGGCCAGACGCCTCTGGATCTGGCAACAGCTGACGATATCAGAGCTTTGCTGATAGATGCCATGCC CCCAGAGGCCTTACCTACCTGTTTTAAACCTCAGGCTACTGTAGTGAGTGCCTCTCTGATCTCACCAGCA TCCACCCCCTCCTGCCTCTCGGCTGCCAGCAGCATAGACAACCTCACTGGCCCTTTAGCAGGGTTGGCCG TAGGAGGAGCCTCCAATGCAGGGGATGGCGCCGCGGGAACAGAAAGGAAGGAAGGAGAAGTTGCTGGTCT TGACATGAATATCAGCCAATTTCTAAAAAGCCTTGGCCTTGAACACCTTCGGGATATCTTTGAAACAGAA CAGATTACACTAGATGTGTTGGCTGATATGGGTCATGAAGAGTTGAAAGAAATAGGCATCAATGCATATG GGCACCGCCACAAATTAATCAAAGGAGTAGAAAGACTCTTAGGTGGACAACAAGGCACCAATCCTTATTT GACTTTTCACTGTGTTAATCAGGGAACGATTTTGCTGGATCTTGCTCCAGAAGATAAAGAATATCAGTCA GTGGAAGAAGAGATGCAAAGTACTATTCGAGAACACAGAGATGGTGGTAATGCTGGCGGCATCTTCAACA GATACAATGTCATTCGAATTCAAAAAGTTGTCAACAAGAAGTTGAGGGAGCGGTTCTGCCACCGACAGAA GGAAGTGTCTGAGGAGAATCACAACCATCACAATGAGCGCATGTTGTTTCATGGTTCTCCTTTCATTAAT GCCATTATTCATAAAGGGTTTGATGAGCGACATGCATACATAGGAGGAATGTTTGGGGCCGGGATTTATT TTGCTGAAAACTCCTCAAAAAGCAACCAATATGTTTATGGAATTGGAGGAGGAACAGGCTGCCCTACACA CAAGGACAGGTCATGCTATATATGTCACAGACAAATGCTCTTCTGTAGAGTGACCCTTGGGAAATCCTTT CTGCAGTTTAGCACCATAAAAATGGCCCACGCGCCTCCAGGGCACCACTCAGTCATTGGTAGACCGAGCG TCAATGGGCTGGCATATGCTGAATATGTCATCTACAGAGGAGAACAGGCATACCCAGAGTATCTTATCAC TTACCAGATCATGAAGCCAGAAGCCCCTTCCCAGACCGCAACAGCCGCAGAGCAGAAGACCTAG Clone variation with respect to NM_003747.2 10:g=>t 248:a=>c 522:t=>a 600:a=>g 1386:g=>a 1686:a=>g 2283:g=>a 3002:g=>a 3798:a=>g |
| Restriction Sites | Please inquire |
| ACCN | NM_003747 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_003747.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003747.1, NP_003738.1 |
| RefSeq Size | 9599 bp |
| RefSeq ORF | 3984 bp |
| Locus ID | 8658 |
| UniProt ID | O95271 |
| Domains | SAM, ANK |
| Gene Summary | Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:10988299, PubMed:11739745, PubMed:16076287, PubMed:19759537, PubMed:21478859, PubMed:22864114, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed:22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed:10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed:16076287). Stimulates 26S proteasome activity (PubMed:23622245).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210517 | TNKS (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS) |
CNY 14784.00 |
|
| RC210517L1 | Lenti-ORF clone of TNKS (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS) |
CNY 17184.00 |
|
| RC210517L2 | Lenti-ORF clone of TNKS (mGFP-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS) |
CNY 15110.00 |
|
| RC210517L3 | Lenti-ORF clone of TNKS (Myc-DDK-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS) |
CNY 15110.00 |
|
| RC210517L4 | Lenti-ORF clone of TNKS (mGFP-tagged)-Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS) |
CNY 17184.00 |
|
| RG210517 | TNKS (tGFP-tagged) - Human tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (TNKS) |
CNY 16384.00 |
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