MED13L (NM_015335) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MRFACD; PROSIT240; THRAP2; TRAP240L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309811 representing NM_015335. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTGCGGCAGCGAACTGGGTGGCGAACGGGGCGAGCCTGGAGGATTGTCACTCCAACCTCTTTTCG CTGGCTGAACTCACGGGAATCAAATGGCGTAGGTACAATTTTGGAGGGCATGGGGACTGTGGACCCATA ATTTCAGCCCCAGCCCAAGATGATCCAATTCTGTTAAGTTTCATCCGCTGTCTGCAAGCTAACCTGCTT TGTGTATGGCGTCGTGATGTCAAACCAGATTGCAAAGAGTTATGGATATTCTGGTGGGGAGATGAACCC AACCTAGTGGGTGTAATACATCATGAACTGCAGGTTGTGGAAGAAGGACTCTGGGAAAATGGCCTTTCC TATGAATGTAGGACGCTGCTCTTCAAAGCGATCCACAATCTGTTAGAAAGGTGCCTAATGGATAAGAAC TTCGTTAGGATTGGGAAATGGTTTGTCCGACCCTACGAAAAGGATGAAAAGCCAGTCAACAAAAGTGAG CATTTGTCCTGTGCTTTCACATTCTTTCTGCATGGAGAAAGTAATGTATGCACAAGTGTGGAGATTGCC CAGCACCAGCCAATTTATTTGATCAATGAGGAGCATATACACATGGCTCAGTCTTCACCTGCACCATTT CAAGTACTGGTAAGTCCTTATGGCTTAAATGGGACGCTAACAGGCCAAGCATACAAGATGTCAGACCCA GCCACTCGTAAGTTGATTGAGGAATGGCAGTATTTCTACCCGATGGTGCTAAAAAAGAAAGAAGAATCG AAAGAGGAAGACGAGTTGGGATATGATGATGATTTCCCTGTGGCAGTTGAAGTAATTGTTGGTGGTGTT CGGATGGTTTACCCTTCAGCATTTGTTTTGATCTCTCAGAATGACATCCCGGTTCCTCAGAGTGTTGCC AGTGCTGGAGGCCACATTGCAGTTGGGCAGCAAGGGCTTGGTAGTGTGAAGGACCCAAGTAACTGTGGG ATGCCTCTGACCCCTCCCACCTCTCCAGAACAGGCTATCCTAGGTGAGAGTGGAGGTATGCAGAGTGCT GCCAGTCACCTGGTTTCCCAAGATGGAGGGATGATAACGATGCACAGTCCAAAGAGATCGGGGAAGATT CCTCCAAAACTCCACAATCATATGGTCCATCGAGTCTGGAAGGAATGCATCCTCAACAGAACCCAGTCC AAGAGGAGCCAAATGTCAACTCCAACTCTTGAAGAAGAGCCTGCTAGCAATCCTGCTACTTGGGATTTT GTGGATCCAACCCAAAGAGTCAGCTGTTCTTGTTCCAGGCATAAGCTTTTAAAACGTTGTGCAGTCGGG CCCAATCGACCTCCCACAGTATCTCAACCAGGGTTCAGTGCAGGACCATCATCATCTTCATCTTTACCA CCTCCTGCTTCTTCTAAGCACAAAACAGCAGAAAGACAGGAAAAAGGAGACAAGCTGCAAAAGAGACCC TTAATACCATTTCACCATAGGCCCTCTGTGGCCGAAGAATTATGCATGGAGCAAGATACACCAGGACAG AAACTAGGGTTGGCAGGGATAGACTCCTCCTTAGAGGTGTCTAGCAGTAGGAAATATGATAAGCAAATG GCCGTGCCTTCCAGAAATACAAGCAAGCAAATGAATCTGAATCCTATGGATTCACCTCATTCCCCTATA TCCCCTCTGCCACCAACACTCAGCCCTCAGCCACGAGGTCAGGAAACAGAGAGTTTGGACCCACCATCG GTCCCTGTGAATCCAGCCCTTTATGGAAATGGACTAGAACTCCAGCAGTTGTCTACTCTGGATGACAGA ACTGTCCTCGTAGGCCAAAGACTGCCTCTCATGGCAGAGGTCAGCGAGACAGCCTTATATTGTGGGATT AGGCCCTCGAACCCGGAGTCATCAGAAAAGTGGTGGCATAGTTATCGTCTCCCACCCAGTGATGATGCT GAGTTCAGGCCTCCAGAGCTCCAGGGTGAGAGATGTGATGCCAAAATGGAGGTAAACTCAGAGAGCACT GCATTGCAAAGACTCTTAGCACAACCTAACAAACGGTTTAAAATCTGGCAAGACAAACAGCCCCAGTTG CAGCCACTCCACTTCCTTGACCCATTGCCTCTATCACAACAACCTGGAGACAGTTTGGGAGAAGTGAAT GACCCATATACCTTTGAAGATGGTGACATAAAATACATCTTTACAGCCAACAAGAAATGCAAACAAGGG ACGGAGAAAGATTCCCTGAAAAAGAATAAGTCAGAGGATGGATTTGGTACCAAGGATGTCACTACACCA GGTCATTCCACGCCGGTGCCTGATGGGAAAAATGCCATGTCTATTTTCAGTTCTGCTACTAAAACAGAT GTCCGGCAGGATAATGCTGCTGGCAGAGCTGGCTCCAGTAGCCTTACACAGGTAACAGATTTGGCACCT TCCCTGCATGACTTAGACAACATCTTTGATAATTCTGATGACGACGAACTTGGGGCTGTATCACCTGCT CTGCGCTCATCAAAAATGCCTGCAGTTGGGACAGAAGACCGACCTCTTGGGAAGGATGGAAGAGCTGCT GTTCCTTATCCACCAACAGTTGCAGACTTGCAAAGGATGTTTCCCACTCCACCATCTTTGGAACAGCAT CCTGCATTTTCTCCTGTGATGAATTATAAAGATGGGATCAGCTCAGAGACAGTGACAGCATTAGGCATG ATGGAGAGCCCTATGGTCAGTATGGTTTCAACACAACTCACAGAATTCAAAATGGAAGTGGAAGATGGA TTAGGAAGTCCCAAGCCCGAGGAAATTAAGGACTTTTCATATGTGCACAAAGTTCCATCCTTTCAACCT TTTGTGGGATCCTCCATGTTTGCTCCACTGAAGATGTTGCCGAGCCATTGTTTGCTACCTCTGAAGATA CCTGATGCCTGTCTGTTTCGGCCTTCATGGGCAATTCCTCCTAAAATTGAACAACTGCCCATGCCCCCT GCAGCCACTTTCATTAGAGATGGCTACAATAACGTGCCTAGTGTTGGGAGCCTAGCAGATCCAGACTAT CTGAACACACCACAGATGAACACACCCGTGACGTTGAACAGCGCTGCCCCAGCCAGCAATAGTGGGGCA GGAGTCCTACCATCTCCAGCAACCCCTCGCTTCTCTGTCCCCACACCACGAACCCCCAGGACCCCAAGA ACTCCCAGAGGTGGGGGCACTGCCAGTGGTCAAGGGTCTGTTAAGTATGATAGCACCGATCAAGGATCA CCAGCCTCCACCCCCTCTACTACACGGCCCCTCAACTCTGTGGAGCCCGCCACCATGCAGCCAATTCCC GAAGCCCACAGCCTCTATGTTACCCTGATTCTCTCCGATTCCGTGATGAATATCTTTAAAGACAGAAAC TTTGACAGCTGTTGCATCTGTGCCTGCAACATGAACATCAAAGGGGCGGATGTCGGGCTTTACATCCCC GATTCTTCCAATGAGGACCAGTACCGCTGTACCTGTGGGTTTAGTGCGATTATGAACCGCAAACTTGGC TACAATTCAGGACTCTTCCTTGAAGATGAGTTGGATATTTTTGGGAAGAATTCTGATATTGGTCAGGCT GCAGAGAGGCGCTTAATGATGTGTCAGTCCACCTTCCTTCCTCAGGTGGAAGGAACCAAAAAACCCCAG GAGCCACCCATAAGCCTTCTCCTCCTCCTCCAGAATCAACACACACAACCTTTTGCTTCACTGAATTTC CTGGACTACATTTCCTCTAACAATCGCCAAACTCTTCCCTGTGTAAGCTGGAGTTATGACCGGGTGCAA GCAGATAATAATGATTACTGGACGGAATGCTTTAATGCGTTGGAGCAGGGGCGGCAGTATGTGGATAAC CCCACTGGTGGAAAAGTGGACGAAGCTCTGGTGAGAAGTGCCACTGTGCACTCTTGGCCTCACAGCAAT GTGCTGGACATCAGCATGCTCTCCTCCCAGGATGTGGTTCGTATGCTGTTGTCCCTGCAGCCCTTTCTC CAAGATGCCATCCAAAAGAAGCGCACGGGCAGGACCTGGGAGAACATCCAGCATGTGCAGGGACCACTC ACTTGGCAGCAGTTCCATAAAATGGCAGGACGGGGAACCTACGGTTCGGAAGAATCTCCTGAGCCGTTG CCCATCCCCACTCTGCTGGTAGGCTATGACAAGGATTTCCTCACCATCTCGCCATTCTCCTTGCCGTTT TGGGAGAGGCTCTTGTTGGACCCATATGGGGGCCACCGTGATGTTGCCTATATTGTGGTGTGTCCAGAA AATGAGGCCTTGCTCGAAGGAGCCAAAACTTTCTTCAGGGACTTGAGTGCTGTATACGAGATGTGTAGG CTTGGGCAGCACAAGCCCATCTGCAAAGTGCTACGTGACGGGATCATGCGCGTGGGAAAAACTGTGGCA CAGAAGCTGACAGATGAGCTTGTGAGTGAGTGGTTTAACCAGCCTTGGAGCGGCGAGGAGAATGACAAT CATTCCAGACTCAAACTTTATGCGCAAGTTTGCCGCCATCACCTAGCACCTTATTTAGCCACTCTGCAG CTTGATAGCAGCCTATTGATACCACCTAAATACCAGACCCCACCAGCAGCAGCACAGGGACAAGCTACG CCAGGGAATGCTGGGCCCTTAGCTCCAAATGGATCAGCAGCTCCCCCAGCTGGCAGTGCATTTAATCCC ACCTCGAATAGTAGTTCTACAAATCCTGCAGCAAGTAGTTCTGCATCTGGTTCCTCTGTGCCACCGGTC TCATCGTCTGCCTCTGCTCCTGGTATTAGCCAGATAAGCACTACCTCTTCTTCAGGATTCAGTGGTAGT GTTGGAGGGCAGAACCCCAGCACTGGGGGCATTTCTGCGGATAGAACGCAAGGGAACATAGGCTGTGGT GGAGACACTGACCCTGGGCAGAGCTCTTCTCAGCCCTCACAGGATGGACAAGAGAGTGTTACAGAAAGG GAGAGAATAGGAATTCCCACGGAGCCTGACTCTGCAGACAGCCATGCCCACCCTCCAGCTGTTGTCATT TACATGGTGGACCCGTTCACGTATGCTGCAGAGGAGGACTCCACTTCTGGGAACTTTTGGCTGTTGAGC TTGATGCGCTGCTACACAGAAATGCTGGATAATTTACCTGAGCATATGAGAAATTCTTTCATTCTCCAG ATTGTGCCTTGCCAGTACATGCTGCAGACAATGAAGGATGAGCAAGTTTTCTACATTCAATACTTGAAG TCCATGGCATTTTCAGTGTACTGCCAGTGCAGGCGACCACTGCCTACACAGATCCACATTAAATCCCTC ACGGGATTTGGGCCTGCAGCCAGCATTGAGATGACCCTCAAGAACCCTGAGCGGCCCAGCCCAATCCAG CTTTACTCCCCTCCCTTTATATTGGCCCCAATCAAAGACAAGCAGACAGAGCTGGGAGAGACGTTTGGT GAGGCGAGCCAGAAATACAATGTGCTCTTCGTGGGCTATTGTCTGTCTCACGACCAGCGCTGGCTTTTG GCTTCCTGCACTGACCTCCATGGGGAATTATTAGAGACCTGCGTTGTAAATATTGCTTTACCAAACAGG TCACGGAGGAGTAAAGTATCTGCACGTAAAATTGGACTACAGAAGTTATGGGAGTGGTGCATAGGGATT GTCCAAATGACATCTCTACCCTGGAGAGTTGTAATCGGGCGACTTGGGCGTCTTGGCCATGGGGAGCTT AAAGATTGGAGTATCCTCCTTGGAGAATGTTCACTACAGACAATCAGCAAAAAGCTCAAGGATGTGTGC CGGATGTGTGGAATCTCTGCCGCAGACTCTCCTTCTATCCTTAGTGCCTGCCTGGTTGCCATGGAGCCC CAGGGGTCCTTTGTAGTGATGCCAGATGCTGTCACAATGGGCTCTGTTTTTGGCCGAAGTACTGCACTG AACATGCAGTCATCTCAGCTCAACACCCCTCAAGATGCTTCTTGTACACACATCTTGGTGTTCCCAACA TCATCAACCATCCAGGTGGCTCCAGCCAACTACCCCAATGAAGATGGGTTTAGCCCCAACAATGATGAT ATGTTTGTTGACCTTCCATTCCCAGATGATATGGACAATGATATTGGCATATTAATGACTGGGAACCTC CATTCCTCTCCCAACTCTTCCCCAGTACCCTCCCCAGGCTCTCCTTCTGGAATTGGTGTGGGCTCTCAC TTCCAGCATAGTCGGAGCCAGGGTGAGCGTCTTCTTTCTAGAGAAGCACCAGAGGAGCTAAAGCAGCAG CCCCTGGCCCTTGGGTATTTTGTATCAACTGCCAAAGCTGAGAATCTTCCCCAGTGGTTTTGGTCATCG TGTCCCCAGGCTCAAAACCAGTGCCCTCTCTTCTTAAAGGCTTCGCTGCATCACCACATTTCAGTAGCA CAGACAGACGAACTTCTGCCTGCCAGGAATTCTCAGCGGGTTCCACACCCTCTTGACTCCAAAACCACG TCGGATGTTTTAAGGTTTGTTTTGGAGCAGTACAACGCTCTGTCCTGGCTCACGTGCAATCCGGCCACC CAGGACCGTACTTCCTGCCTTCCCGTCCACTTTGTGGTGCTCACTCAGTTGTACAATGCCATCATGAAT ATACTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015335
Insert Size	6633 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015335.4
RefSeq Size	9393 bp
RefSeq ORF	6633 bp
Locus ID	23389
UniProt ID	Q71F56
Protein Families	Druggable Genome
MW	242.6 kDa
Gene Summary	The protein encoded by this gene is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. The encoded protein is involved in early development of the heart and brain. Defects in this gene are a cause of transposition of the great arteries, dextro-looped (DTGA).[provided by RefSeq, Jul 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.