SKT (KIAA1217) (NM_019590) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ETL4; SKT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309799 representing NM_019590. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGAAAATGAAAGCCAGAAATGTGAGCCGTGCCTTCCTTACTCAGCAGACAGAAGACAGATGCAG GAACAAGGCAAAGGCAATCTGCATGTAACATCACCAGAAGATGCAGAATGCCGCAGAACCAAGGAACGC CTTTCTAATGGAAACAGTCGTGGTTCAGTTTCCAAGTCTTCCCGCAATATCCCAAGGAGACACACCCTA GGGGGGCCCCGAAGTTCCAAGGAAATACTGGGAATGCAAACATCTGAGATGGATCGGAAGAGAGAAGCG TTCCTAGAACATCTGAAGCAGAAGTACCCCCACCACGCCTCTGCAATCATGGGTCACCAAGAGAGGCTG AGAGACCAGACAAGGAGCCCCAAACTGTCTCACAGTCCTCAACCACCCAGTCTGGGTGACCCGGTCGAG CATTTATCAGAGACGTCCGCTGATTCTTTGGAAGCCATGTCTGAGGGGGATGCTCCAACCCCTTTTTCC AGAGGCAGCCGGACTCGTGCGAGCCTTCCTGTGGTGAGGTCAACCAACCAGACGAAAGAAAGATCTCTG GGGGTTCTCTATCTCCAGTATGGAGATGAAACCAAGCAGCTCAGGATGCCGAATGAAATCACAAGTGCA GACACAATCCGTGCTCTCTTCGTAAGTGCCTTTCCACAGCAGCTCACCATGAAAATGCTGGAATCGCCC AGTGTCGCCATTTACATCAAAGATGAAAGCAGAAATGTCTATTATGAATTAAATGATGTAAGGAACATT CAAGACAGATCACTCCTCAAAGTGTACAACAAGGATCCTGCACATGCGTTTAATCACACACCAAAAACT ATGAATGGAGACATGAGGATGCAGAGAGAACTTGTTTATGCAAGAGGAGATGGCCCTGGGGCCCCTCGC CCCGGATCTACTGCTCATCCACCCCATGCGATTCCAAATTCCCCACCGTCTACTCCAGTGCCCCATTCC ATGCCCCCCTCCCCGTCCAGAATTCCTTATGGGGGCACCCGCTCCATGGTTGTTCCTGGCAATGCCACC ATCCCCAGGGACAGAATCTCCAGCCTGCCAGTCTCCAGACCCATCTCTCCAAGCCCAAGCGCCATTTTA GAAAGAAGAGATGTCAAGCCTGATGAAGACATGAGTGGCAAAAACATTGCAATGTACAGAAATGAGGGT TTCTATGCTGATCCTTACCTTTATCACGAGGGACGGATGAGCATAGCCTCATCCCATGGTGGACACCCA CTGGATGTCCCCGACCACATCATTGCATATCACCGCACCGCCATCCGGTCAGCGAGTGCTTATTGTAAC CCCTCAATGCAAGCGGAAATGCATATGGAACAATCACTGTACAGACAGAAATCAAGGAAATATCCGGAT AGCCATTTGCCTACACTGGGCTCCAAAACACCCCCTGCCTCTCCTCACAGAGTCAGTGACCTGAGGATG ATAGACATGCACGCTCACTATAATGCCCACGGCCCCCCTCACACCATGCAGCCAGACCGGGCCTCTCCG AGCCGCCAGGCCTTTAAAAAGGAGCCAGGCACCTTGGTGTATATAGAAAAGCCACGGAGCGCTGCAGGA TTATCCAGCCTTGTAGACCTCGGCCCTCCTCTAATGGAGAAGCAAGTTTTTGCCTACAGCACGGCGACA ATACCCAAAGACAGAGAGACCAGAGAGAGGATGCAAGCCATGGAGAAACAGATTGCCAGTTTAACTGGC CTTGTTCAGTCTGCGCTTTTTAAAGGGCCCATTACAAGTTATAGCAAAGATGCGTCTAGCGAGAAAATG ATGAAAACCACAGCCAACAGGAACCACACAGATAGTGCAGGAACGCCCCATGTGTCTGGTGGGAAGATG CTCAGTGCTCTGGAGTCCACGGTGCCTCCCAGCCAGCCTCCACCTGTGGGCACCTCAGCCATCCACATG AGCCTGCTTGAGATGAGGCGGAGCGTGGCGGAACTCAGGCTCCAGCTCCAGCAGATGCGGCAGCTCCAG CTGCAGAACCAGGAGTTGCTGAGGGCAATGATGAAGAAGGCCGAGCTGGAAATCAGTGGCAAAGTGATG GAAACAATGAAGAGACTGGAGGATCCCGTGCAGCGACAGCGCGTCCTAGTGGAGCAAGAGAGACAAAAA TATCTTCATGAGGAAGAGAAGATCGTCAAGAAGTTGTGCGAGTTGGAAGACTTTGTTGAAGACTTGAAG AAGGACTCCACGGCAGCCAGCCGATTGGTTACTCTGAAAGACGTGGAAGACGGGGCTTTCCTCCTGCGT CAAGTGGGAGAGGCTGTAGCTACCCTGAAAGGAGAATTTCCAACCTTACAAAACAAGATGCGAGCCATC CTGCGCATAGAAGTGGAGGCCGTGCGGTTTCTGAAGGAGGAGCCACACAAGCTGGACAGTCTCCTGAAG CGTGTGCGCAGCATGACAGACGTCCTGACCATGCTGCGGAGACATGTCACTGATGGGCTCCTGAAAGGC ACGGACGCAGCCCAAGCCGCACAGTACATGGCTATGGAAAAGGCCACAGCCGCAGAAGTCCTGAAGAGT CAGGAGGAGGCAGCCCACACCTCCGGCCAGCCCTTCCACAGCACAGGTGCCCCTGGCGATGCGAAGTCG GAAGTGGTGCCTTTGTCCGGCATGATGGTTCGCCACGCGCAGAGCTCCCCTGTGGTCATCCAGCCCTCC CAGCACTCCGTGGCCCTGCTGAACCCTGCTCAGAACTTGCCTCACGTGGCCAGCTCCCCAGCCGTCCCC CAGGAAGCAACCTCCACTCTGCAGATGTCGCAGGCTCCGCAGTCCCCACAGATACCCATGAATGGGTCT GCCATGCAGAGCTTGTTCATTGAAGAAATCCACAGTGTGAGTGCCAAGAACAGGGCAGTGTCTATCGAG AAAGCAGAAAAGAAATGGGAGGAAAAAAGGCAAAATCTGGATCACTATAATGGGAAAGAGTTTGAGAAG CTCCTAGAAGAAGCTCAGGCCAATATCATGAAGTCAATACCAAATCTGGAGATGCCGCCAGCCACAGGC CCACTGCCAAGGGGAGATGCCCCAGTGGACAAGGTGGAACTTTCAGAAGATTCTCCAAATTCGGAACAG GACTTGGAAAAGCTGGGGGGAAAGTCGCCCCCTCCTCCTCCGCCACCTCCTCGTCGAAGCTACCTGCCA GGATCGGGACTCACCACCACGAGGTCAGGCGATGTGGTCTACACCGGCAGAAAGGAGAACATCACCGCT AAGGCAAGCAGTGAAGATGCTGGACCAAGCCCACAGACCAGAGCTACAAAATATCCAGCAGAGGAGCCT GCTTCAGCCTGGACCCCATCCCCACCGCCTGTCACCACCTCCTCCTCAAAGGATGAGGAGGAAGAAGAA GAAGAAGGAGACAAAATAATGGCAGAACTCCAGGCATTCCAGAAGTGTTCCTTTATGGATGTAAATTCA AACAGTCATGCTGAGCCATCCCGGGCTGACAGTCACGTTAAAGACACTAGGTCGGGCGCCACAGTGCCA CCCAAGGAGAAGAAGAATTTGGAATTTTTCCATGAAGATGTACGGAAATCTGATGTTGAATATGAAAAT GGCCCCCAAATGGAATTCCAAAAGGTTACCACAGGGGCTGTAAGACCTAGTGACCCTCCTAAGTGGGAA AGAGGAATGGAGAATAGTATTTCTGATGCATCAAGAACATCAGAATATAAAACTGAGATCATAATGAAG GAAAATTCCATATCCAATATGAGTTTACTCAGAGACAGTAGAAACTATTCCCAGGAAACTGTGCCTAAG GCCAGTTTCGGTTTCTCTGGCATTAGTCCATTAGAAGATGAAATAAACAAAGGGTCTAAAATCTCAGGC CTGCAATACTCTATACCTGACACCGAGAACCAGACGCTGAATTACGGAAAGACAAAGGAGATGGAAAAG CAAAATACGGATAAGTGTCACGTTTCCTCTCACACTAGACTAACAGAATCAAGCGTGCATGATTTTAAA ACAGAAGATCAAGAGGTTATCACGACAGATTTTGGCCAAGTTGTTCTAAGACCCAAGGAGGCAAGGCAT GCTAACGTGAACCCTAATGAGGATGGAGAATCAAGTTCAAGTTCTCCCACTGAAGAAAATGCAGCCACT GACAATATTGCCTTCATGATTACCGAAACCACTGTCCAGGTTCTTTCCAGTGGGGAGGTGCATGATATT GTTAGCCAAAAGGGAGAAGACATACAGACGGTTAATATCGATGCCAGAAAAGAGATGACCCCCCGACAA GAAGGGACTGACAATGAGGATCCAGTCGTGTGCCTGGACAAGAAACCAGTGATCATCATTTTCGATGAG CCCATGGACATCCGGTCTGCCTATAAGAGACTTTCAACTATCTTTGAGGAATGTGATGAGGAATTAGAG AGAATGATGATGGAGGAAAAGATAGAGGAGGAGGAAGAGGAGGAAAATGGGGATTCTGTAGTCCAGAAT AATAACACTTCCCAGATGTCTCATAAGAAGGTGGCCCCAGGCAATCTTAGAACCGGACAACAGGTGGAA ACAAAGTCACAGCCACACTCCCTGGCCACAGAGACCAGAAACCCAGGAGGACAGGAAATGAACAGAACG GAGCTGAACAAGTTCAGCCACGTGGATTCTCCAAATTCGGAATGCAAGGGTGAGGACGCGACCGATGAC CAGTTTGAAAGCCCCAAGAAAAAGTTTAAATTCAAATTCCCTAAGAAGCAACTCGCCGCTCTCACTCAA GCCATTCGCACCGGAACTAAAACAGGGAAGAAGACTTTGCAAGTGGTAGTCTATGAAGAAGAGGAAGAG GATGGCACCCTGAAACAGCACAAAGAAGCCAAGCGCTTCGAAATCGCTAGGTCTCAACCTGAAGACACC CCTGAAAACACAGTGAGGAGGCAAGAGCAGCCCAGCATCGAGAGTACATCTCCGATTTCAAGAACTGAT GAAATTAGAAAAAACACCTACAGAACATTGGATAGCCTGGAGCAGACCATTAAACAGCTCGAAAATACA ATCAGTGAAATGAGTCCCAAAGCCCTAGTTGATACCTCATGTTCTTCCAACAGAGATTCTGTTGCAAGT TCATCCCACATAGCCCAAGAGGCCTCTCCCCGACCCTTGCTAGTTCCGGATGAAGGTCCCACTGCCCTA GAGCCCCCTACGTCGATACCTTCAGCTTCACGTAAGGGCTCCAGCGGGGCCCCACAGACGAGCAGGATG CCTGTCCCCATGAGTGCCAAGAACAGACCCGGAACCCTGGACAAACCCGGCAAGCAGTCCAAACTGCAG GATCCCCGCCAATATCGTCAGGCTAATGGAAGTGCTAAGAAATCTGGTGGGGACTTTAAGCCTACTTCC CCCTCCTTACCTGCTTCTAAGATTCCAGCCCTTTCTCCCAGCTCTGGGAAAAGCAGTTCTCTGCCCTCT TCTAGTGGTGACAGCTCTAACCTCCCTAATCCACCTGCTACTAAACCATCGATTGCTTCTAACCCTCTC AGCCCCCAAACAGGACCACCTGCTCACTCTGCCTCCCTCATCCCTTCTGTCTCTAATGGCTCTTTGAAG TTTCAGAGCCTCACTCATACAGGTAAAGGTCACCATCTTTCATTCTCACCGCAGAGTCAAAATGGCCGA GCACCCCCTCCTTTGTCATTTTCCTCCTCCCCTCCTTCTCCTGCCTCCTCCGTCTCACTGAATCAAGGT GCCAAGGGCACCAGGACCATCCATACTCCCAGCCTCACCAGCTACAAGGCACAGAATGGAAGTTCAAGC AAAGCCACCCCATCCACAGCAAAAGAAACCTCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_019590
Insert Size	5832 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019590.4
RefSeq Size	7759 bp
RefSeq ORF	5832 bp
Locus ID	56243
UniProt ID	Q5T5P2
MW	214.1 kDa
Gene Summary	Required for normal development of intervertebral disks.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.