DENND4A (NM_005848) Human Untagged Clone
CAT＃: SC309778
DENND4A (untagged)-Human DENN/MADD domain containing 4A (DENND4A), transcript variant 2
CNY 26790.00
货期*
8周
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IRLB; MYCPBP |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_005848, the custom clone sequence may differ by one or more nucleotides
ATGATTGAAGACAAGGGGCCTCGTGTTGCTGACTACTTTGTTGTAGCAGGATTAACTGAT GTTTCAAAGCCTCTAGAAGAAGAAATTCACTTCAATGATGCTTGTCATAAAGTAGCTAAA CCAAAAGAACCTATTACAGATGTTTCAGTTATTATCAAATCTCTTGGGGAGGAAGTCCCA CAGGATTATATCTGTATTGATGTTACCCCAACTGGATTGTCAGCTGATCTTAATAATGGA AGTCTTGTGGGGCCACAGATTTACCTTTGCTATAGAAGAGGAAGAGATAAGCCTCCACTT ACAGATCTGGGGGTTTTATATGACTGGAAAGAAAGATTGAAACAGGGTTGTGAAATTATT CAGAGTACTCCCTATGGGCGCCCCGCAAATATTAGTGGGAGTACCTCATCACAAAGAATT TATATCACTTACCGAAGAGCCTCTGAAAACATGACTCAGAATACGTTGGCTGTCACTGAC ATATGTATTATTATACCCAGTAAAGGAGAAAGCCCACCACACACGTTCTGCAAAGTCGAC AAGAATCTCAATAACAGTATGTGGGGATCAGCAGTATACTTGTGTTATAAAAAATCGGTG GCAAAGACGAACACTGTATCTTACAAAGCTGGCCTAATTTGTAGATATCCTCAAGAAGAT TATGAGTCATTCTCACTACCGGAATCTGTTCCTCTATTTTGTTTACCAATGGGTGCAACC ATTGAATGTTGGCCATCAAATAGCAAATACCCTCTGCCTGTATTTTCTACTTTTGTTTTA ACTGGAGCCTCAGCTGAAAAGGTTTATGGTGCTGCTATTCAGTTTTATGAACCATACTCT GAGGAGAATCTCACAGAAAAGCAGAGACTTCTTTTGGGTTTAACATCAGCAGATGGGAAG TCTGATAGTTCCAAAACAATTCATACTAACAAATGCATCTGTCTTCTTTCTCACTGGCCT TTTTTTGATGCATTCAGGAAGTTTCTGACTTTTCTGTATCGTTATTCCATCTCTGGGCCT CATGTCCTTCCAATTGAGAAGCATATTTCTCATTTTATGCATAAAGTTCCTTTTCCATCT CCTCAGAGACCACGGATTTTAGTTCAGTTATCACCACATGATAATCTGATTCTCAGTCAG CCTGTGTCTTCACCTCTTCCACTAAGTGGTGGCAAGTTTTCAACACTACTGCAGAATTTA GGCCCTGAAAATGCTGTGACACTACTGGTGTTTGCAGTAACAGAACATAAAATTCTTATC CATTCCCTACGGCCATCCGTGCTTACTAGTGTGACAGAAGCATTAGTGTCTATGATTTTC CCTTTCCACTGGCCATGCCCGTATGTTCCTCTCTGCCCACTGGCTTTAGCAGATGTCTTG AGTGCACCATGTCCATTCATAGTAGGGATTGATTCAAGATACTTTGATCTCTATGATCCT CCACCAGATGTCAGTTGTGTTGATGTAGATACCAACACTATTTCTCAAATTGGTGACAAG AAAAATGTAGCCTGGAAGATACTTCCAAAGAAGCCATGTAAAAATCTGATGAACACACTG AATAATTTGCATCAGCAATTGGCAAAATTGCAGCAGAGACCGAGAGATGATGGACTCATG GATTTAGCAATAAATGACTATGATTTTAATTCTGGAAAGAGGTTGCACATGATAGATTTG GAAATCCAAGAGGCATTTTTGTTTTTCATGGCCTCTATTTTAAAAGGTTACAGATCATAC TTAAGGCCAATAACACAAGCCCCATCTGAGACAGCCACAGATGCAGCCTCTCTCTTTGCC CTACAAGCTTTCTTAAGAAGCCGGGACCGGTCACATCAAAAATTCTATAACATGATGACC AAAACACAAATGTTTATTCGCTTCATTGAAGAATGTTCTTTTGTTAGTGACAAAGATGCA AGCCTGGCATTTTTTGATGATTGTGTAGATAAAGTGGATATGGACAAGAGCGGTGAAGTA CGACTTATAGAACTGGATGAATCTTTCAAAAGTGAACACACTGTTTTTGTAACACCACCT GAGATCCCCCACCTACCCAATGGTGAAGAACCCCCTTTACAGTACAGCTATAATGGATTT CCAGTTCTTAGGAACAATTTATTTGAAAGACCTGAAGGATTTCTACAAGCAAAGAAGAAC AAATTGCCTTCAAAATCAAGTAGTCCTAATAGCCCTTTGCCCATGTTCAGAAGAACAAAA CAGGAAATTAAATCAGCCCATAAAATTGCAAAGAGGTACTCTTCCATCCCTCAGATGTGG TCTAGGTGTCTACTGCGCCACTGTTATGGACTGTGGTTTATTTGTCTCCCAGCTTATGTG AAAGTCTGTCATTCAAAAGTCAGGGCTCTGAAAACAGCATATGATGTGCTTAAAAAAATG CAGTCAAAGAAGATGGATCCACCTGATGAGGTATGCTACCGCATTCTTATGCAACTCTGT GGACAATATGATCAGCCAGTGCTTGCAGTTCGAGTGCTTTTTGAAATGCAGAAAGCTGGT ATTGACCCCAATGCCATTACTTATGGTTATTATAATAAGGCTGTTTTGGAAAGTACCTGG CCTTCAAGAAGTCGTAGTGGCTATTTTCTTTGGACAAAAGTAAGAAATGTTGTTTTAGGA GTAACACAGTTCAAAAGAGCTTTAAAGAAGCATGCACACTTATCACAAACAACTCTCTCA GGTGGCCAGTCTGATCTTGGATATAATTCATTATCTAAAGATGAAGTTAGAAGAGGGGAT ACATCTACTGAAGACATTCAAGAAGAAAAAGATAAAAAAGGGAGTGATTGTAGTTCCTTG TCAGAGAGTGAGAGTACAAAAGGAAGTGCTGATTGCCTTCCTAAGCTCAGTTATCAAAAT TCTTCCAGTATCGTTCGCCTCACTGGTACAAGTAACAACAGTGCTGGTAAAATATCAGGA GAAAGCATGGAAAGCACACCTGAGCTGCTCTTAATATCATCTCTTGAAGATACAAATGAA ACACGAAACATTCAAAGTAGATGCTTCAGGAAAAGACATAAAAGTGACAATGAAACTAAT TTGCAGCAGCAAGTGGTCTGGGGAAATAGAAACCGTAATCTTAGTGGAGGGGTACTGATG GGATTTATGCTCAATAGAATTAACCAGGAAGCAACCCCTGGAGATATAGTTGAAAAATTG GGAGCTGATGCAAAAATTCTTTCAAATGTTATCTCAAAAAGCACGAGACCAAATACTCTT GATATTGGGAAGCCACCTTTAAGATCAAAAAGAGACAGTCTAGAAAAGGAATCTAGTGAT GATGATACACCTTTTGATGGTTCTAACTATTTGGCAGATAAAGTGGATTCTCCTGTTATT TTTGACTTAGAAGACTTAGACAGTGAAACAGATGTATCAAAAGCAGGATGTGTTGCTACA CAAAATCCCAAGAGGATTCAACGTATGAACAGCAGCTTTTCAGTAAAACCTTTTGAAAAA ACTGATGTCGCAACAGGATTTGATCCCCTCTCTCTTTTGGTTGCTGAGACTGAACAGCAG CAAAAAGAAGAAGAGGAGGAGGATGAAGATGATAGCAAAAGCATTTCAACACCATCTGCT AGGCGTGATTTAGCTGAAGAAATTGTGATGTATATGAATAACATGAGCAGTCCTTTGACA AGTCGTACACCAAGCATTGATTTACAACGGGCATGTGATGATAAATTAAATAAGAAAAGT CCACCATTGGTCAAGGCATGCAGAAGATCTAGTCTGCCTCCTAATTCTCCTAAGCCAGTG AGACTTACCAAATCTAAAAGTTACACAAAAAGTGAGGAGAAACCAAGGGATAGACTTTGG TCTTCTCCAGCCTTCTCACCTACTTGCCCATTTAGGGAAGAATCTCAGGATACATTAACC CACTCATCACCTTCATTTAACTTAGATACACTACTAGTACCTAAACTAGATGTTCTAAGA AACAGTATGTTCACTGCTGGAAAAGGAGTTGCAGAGAAAGCAAGCAAATGGTATTCAAGA TTCACCATGTACACCACATCATCTAAGGATCAAAGTTCTGATCGTACCAGTCTTTCTTCA GTGGGAGCCCAGGATTCTGAATCTACCTCTTTAACAGATGAAGATGTCTGCCATGAGTTG GAAGGACCTATCTCCTCTCAAGAGACCAGTGCTACTTCAGGGACTAAGAGAATTGATCTC AGCCGAATAAGCCTGGAAAGTTCTGCATCCTTGGAAGGATCTCTGTCGAAGTTTGCCTTA CCTGGGAAATCAGAAGTGACATCTTCCTTCAACGCGAGTAATACAAATATCTTCCAGAAC TATGCAATGGAGGTTCTCATCTCAAGTTGCTCTCGGTGTAGAACTTGTGATTGTCTTGTC CATGATGAGGAAATCATGGCTGGCTGGACAGCAGATGATTCAAATCTCAATACTACATGC CCATTCTGTGGCAATATCTTCTTACCCTTTCTGAATATAGAAATAAGAGATTTAAGACGA CCTGGAAGATACTTTCTAAAGTCAAGCCCATCAACAGAAAATATGCACTTTCCATCCTCC ATTTCAAGTCAGACGAGGCAGTCTTGCATTTCAACATCAGCCTCTGGTCTTGACACATCT GCTCTCTCTGTTCAAGGGAATTTTGATCTAAATAGCAAATCTAAACTGCAGGAAAATTTT TGCACCCGAAGTATTCAGATCCCTGCTAATAGATCAAAAACAGCTATGTCTAAATGTCCA ATATTTCCAATGGCCAGGAGTATTAGTACTTCTGGCCCCTTGGATAAGGAAGATACTGGA AGACAGAAGCTCATTTCTACAGGCAGCCTGCCAGCTACTTTACAAGGAGCTACAGATTCT TTAGGTTTAGAATGGCACCTTCCAAGTCCCGATCCTGTCACTGTTCCGTATCTTAGTCCT TTAGTGGTATGGAAAGAACTTGAAAGCTTATTGGAAAATGAAGGTGATCATGCAATAACA GTAGCAGACTTTGTGGACCATCATCCAATTGTTTTTTGGAACCTGGTATGGTATTTCAGA CGTCTTGACTTGCCCAGTAACTTACCTGGATTGATTCTTTCTTCTGAGCACTGTAACAAG TATTCAAAGATTCCCCGCCACTGTATGTCTGAAGATAGCAAATATGTTTTAATACAAATG TTATGGGACAATATGAAGTTACATCAGGATCCGGGACAGCCCTTGTACATTCTCTGGAAT GCACACACCCAAAAATATCCAATGGTCCATTTATTGCAAAAAAGTGATAACTCATTTAAC CAGGAACTGTTGAAAAGTATGGTAAAAAGCATTAAAATGAATGATGTCTATGGACCAATG AGTCAGATTTTAGAGACACTGAATAAGTGTCCACATTTTAAAAGACAGAGGAGTTTATAC AGAGAAATACTATTTCTCTCACTTGTGGCACTGGGAAGAGAAAACATTGATATAGATGCT TTTGATAAAGAGTACAAGATGGCATACGATCGTCTCACACCTAGTCAAGTCAAGAGTACA CACAACTGTGATAGACCACCAAGTACAGGGGTGATGGAATGTCGAAAAACCTTTGGAGAA CCTTATCTTTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_005848 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005848.1, NP_005839.1 |
| RefSeq Size | 6585 bp |
| RefSeq ORF | 5598 bp |
| Locus ID | 10260 |
| UniProt ID | Q7Z401 |
| Gene Summary | This gene encodes a DENN domain-containing protein that may function as a guanine nucleotide exchange factor that specifically activates ras-related protein Rab-10. This protein also contains a interferon stimulated response element-binding domain and may be involved in regulating the v-myc avian myelocytomatosis viral (MYC) oncogene. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
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