MCM10 (NM_182751) Human Untagged Clone
CAT＃: SC309663
MCM10 (untagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1
CNY 9624.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CNA43; DNA43; PRO2249 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Restriction Sites | NotI-NotI |
| ACCN | NM_182751 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182751.1, NP_877428.1 |
| RefSeq Size | 4535 bp |
| RefSeq ORF | 2628 bp |
| Locus ID | 55388 |
| UniProt ID | Q7L590 |
| Protein Families | Stem cell - Pluripotency |
| Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) uses a different splice site, compared to variant 2, that results in a longer isoform (1). Though this variant is supported by EST data, the existence of its encoded isoform has not been experimentally verified. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215642 | MCM10 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1 |
CNY 9608.00 |
|
| RC215642L3 | Lenti-ORF clone of MCM10 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1 |
CNY 5890.00 |
|
| RC215642L4 | Lenti-ORF clone of MCM10 (mGFP-tagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1 |
CNY 5890.00 |
|
| RG215642 | MCM10 (tGFP-tagged) - Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1 |
CNY 4370.00 |
