Rotatin (RTTN) (NM_173630) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MSSP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309199 representing NM_173630. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTCCTGGCAGGGCTCATCAGGAAACTCGGTCATCAGCTGGCCGAGATCAGGGAGCGCGCTCTCAAG AGTATTCTCTGCAAGATTGAGCACAACTTAATCTGCTACGCTGATCTCATTCAGGAGAGGCAACTTTTT CTTCATTTGCTGGAATGGTTCAATTTCCCGTCCGTTCCGATGAAGGAAGAGGTTCTGAACCTGTTAAGC AGATTGGTTAAGTATCCCCCAGCAGTCCAACATTTGGTTGACGTTGGTGCAGTAGAGTTCTTATCTAAG CTTCGGTCTAATGTGGAGCCAAATCTGCAGGCTGAAATTGATGGCATTCTGGATGGACTTTTTCTTCTT CCTTCGGAAGTTCCTGCACTATCTTCTGCCTCATACCAAACCAATCAAACTGAATTGTCAAAAAACCCT GAAATCTTAACAGGATATTTTCCCCAAGACAAAAGTAATTTCCAGCAGATGGAAGTGCCGCCACGACCA GTGGTAAATCAGACTGTGAAGTGCTTGAAGTTTTCTACATTTCCTTGGCTACCCCTGACCACCACAGAC AGACATGTCCTCTCCTCTAATGAAAGCTCTTTAAGAAGTAGTAACCACACTTTAATCTGGAACACCTGT GAACTATTGAAGGATGTTATCATGCAAGATTTTCCTGCTGAGATTTTCCTTCAAAGGCCAAAAATTGTT CAGAGCCTTTTATCTCTGTTGAAACTGGCCTTTGGAGATGGAAAGCATCGCCTGGCATTACAGTCGGTG TCCTGCCTGCAGCAGCTGTGCATGTATTTAAGAAACAGACTTAACTTTCACCGAGATCCAGGTTTTTTC TCCAATAAACACGACACAGTTTCCCAAAATTCTTCTTTGTCTTATTGTCATGAAGCAAGAGGTACTCAT CATTCCCAGAATCCTTCCCCAGGAAGCAGCAGCCCTCGGCCTTCTGTGGTTGGGCGCACAGGCCAGCGA CCCAGAGGAGATGGCCAGGACTGGGATGCAGCGTCCTCTAGTGGAAGTAGTAGTCATGCTCATGTAAAC TCCAGGATATCCGTTCATTCACCTTTGGATATGGGACACATAGATCTGCCAGAGCTGGAAACTGAAGAC ACATTGGAACTACAATTCCAGCAGCTCAGTCTTCCCCAGTTTTGTGTCTCCATTCTGGAATCAGCTGTT CCTCTCTTAAGAACAGGTAGCAGACAAGTGATAATAAGAGTTCTGGAATTGCTTACAGAGGATATGACA CTTATTGGTGAAGCAATTTCAACAGATATCTGGGATGACAGCAGCCTTTTTGGTATAGATATGAAGGAG AAACTACTCCTGGTGTTGGGAGCCCTTGGAGAAACCATGTGCTACCATAAAAGTAGTATCAGTTTGGAG CAGCCAGAGGTGATGCTTGTGCACCACAGAATGGCCTTTATCAGCATTTCCCTGTTTGCAGTTCGACTG CTACAAACGCTTCTCCCTGTTGAAAAGGCAAGCGAGTTTTTATCAGAGCCTATGTCAACAGCATTATTT CTCCTTTCTTTGGACATGCCTATTTCTTTGGAATATCCAAATATTCATGAAGCTGTTGTGGCCTATTTG GAACAGCTGAATTCTGAAAACTACAGTATTTATAAACGAACTGCAGAGGCCGTTTATTCAATTGAATGC ACCTGTAACTTCCTGTCTGATATTGGGAAAGAGGGAGAGAAGAATCTCTTAGAATTAGTGGAGCTGGCA GACCAAGCCTTGCGTAGCTTTTCCTATCATCAGCATTTCCCGCTAATAAAGGAAATCATCAGCATTTGT TCAAAGATCTGGAAATCTGCTCAGGCCAGTCCATTACTACAAGGAGAAAGTCAGAAGGTGCTTCTCCAT ATGTTGTCTCACCCATTGCCACGAGTGAAAGCTGAAACTTACCACTGCTGTCTGGAAATCACGAAGGAA TGTTTAGGTGTCCATAATGTCACTAAACCCGTGTCTTCACTTTGCAATGGAATTCATTTTCTACTTCAT CCAAAAGTTCTGTATGAAATCTCTGTATTTGGCATTCAAGAGCCCGAAAGTGAGGTGAACACTGCTGCC AAGGCCATTCTGTTATACCTGCTTCAGGGACGATTGATGATGACAGCATTGACCTGGAACAAGTTTATT GAATCTCTCTGTCCTGTCATCCCAATACTACAGGGCTATGCCGACACAGAAGATCCTCTGGGTAACTGC ATTCTCCTTCTAAGCAAAGCAAGTTCTGACACAGAAGAGATGCTTCCGTGTACTACCCGATTAAAGTCC ATGCTGCGACTTTTGCTAGTGAAAAAGCCATCGGTCCGTTCGCTAGCATTAAAGCTTTTAGCATTCCAT CTTACCAGTGAAGAAGGGGCTGATACAAAGCGTCCTCTAATAGACGCCAGAGTTCTCTCCAGAGTTACT GATTTATTCATTGGGAAGAAACCTATTGAACTCAGACTGGATGACAGAAGAGAGCTGGTTATTAAGTTG GAGACTGTTGAAAAGGTATATGAAATCTTCACCTCAGATGATGTTGATCTCGTTTTGAGAAAGTCAGCT GCTGAACAGTTAGCTGTGATTATGCAAGATATTAAAATGCATGCTGTGGTGAAAAAGTTATGCTTAATT GACAAAATAATTGAGTATTTAAATGAATGTGTGAGTCAAGATGGCAAGGTTGTAGAATGTTTGGTACAA CCATGCCTCACACTCTTGAGGAAGGTTTTATGTGGTGATCCAGTCATGCGTGTTTCGCTCTCGCAACAG TCTTCTCTTTTGACCGTGTTATTCAGAGTTTCCTTAATATTTCATGAAGATTGTAGTGTCGTGACTGAA GTTGGAGCACTATTTTGTCTTCTATTATTTGATGAAGTATCGAGAATGGATATGTGGTCTGTTAATCCT TCCAATAAACCTTCTTTGCCATCGGTCTTCAGTTTGCCTGTTTCCGTTTTTAGAAGGTACCATCTACCT GTTCATGTAATTGGACACCATGCTGTGAGTCCTTACTCCATAGTTTTGCCCTTATCTGCTGATTGTTTG GCCTTGAAGCCGGTGTCAGATATGCTGAGAATAGCTTGGAACCTGTCATGGTATCATGGGAGTGATAAT CTGTTGAAGCAAATGAACTCTGAAACTAAAACGCAAGAAATTTTAGATGCATTGAAGTTATCTACAGAG GACATCCTCACTCTGAAGATAACACACATGGCTAGTGGGCTGCAGGACTGCCTCCATTCCATTGTTCAG GCTGCAACCCACAGGGAAGTTAGGGCTGCTGTCACCAGGATGAGTTTTTATCTTCTGAATGACAGATTG TCTTTAAAGGGTTGCCCTGGTCCATGTGGGGTTACCTTGAAGTCCTTGGCTTGGCACACCGCACTAAAC AGGTTTTTACAGGTGCTTCCTGCTTGCACTGAAGATGAGAAACTGCTAATAGATATCATACATTTTTTA AATAAATTAATAAAGGAACAAAGAAAAAATTCTTCACTAGAACTTCTAAACTGGATTCTCGAATTACTT CTTAGACATAGTGCAAACCCACTGTTAGACCTCTTGGTTCTGACAGAGTCACAGGCACGAGAAGAAACA GATGATATCCGGACTGCTGTCAGGCAACAACTTCAGAAAGAACTGATTGCTCTTTTTGATACCTTGCTG CTCAATTTCATGGAAGTTACTGACAGGAAATGCTCGGAACTTCTTTACGTTTTTCAAACGCAGCTGGCT CTGAAACTGCTCCAGTGTCTGAAAGTGACGGATGCGCCTCATTTCTATGGCCTGCCGTCCCTTGAGCGG ACCTTACGAGGGATGGCTAACCTCACTGCGTTTCCGGGATGGAGCTCACACTCTCCTCTCACAAAGCCT CTAGATATCTGTGTGAAGTACTTGTCAGGTCTCCTTGAGGTCATTACTTCTTTTTATGTGGAGCGTGGA GGAAATGCTATGTCCTTCATGGGAAAAGGTGTTACAAAGAGCACAATTCTTTGCTTGCTTCACTTATCC CATGAGATGATGGCCCAGGCTGGGAGCTTGGAGTGGATGTCACTTTGGTTCTTGCCTTTGGGTAGTCAT AGTGAAGAACATATTCCTACTCAACAAGGATTGGCTTGGTTGATTCCATTATGGGTTGATCGGGACCCA GAGGTGAGATTCACTTCACTGGGATTAGGATCAGCACTGACCACCCTTGAAACGGGCTGTGTGGCCTTA GCAAACAGTTGTCAGAACATTTCCGGTGGGCTCTGGGGAACTGTGGTGAACATTCTTCTGGACCAGTCA GAATGTAGTATGGTGCGCCGGGAGGCGGCATTTATTCTTCAGAATCTCCTTGTAATTCCAATGCCTACA GAAATTATAAAGGATTATACTTGGCAGGGTCCCTGTGTTCATGATGAGGACTCTGGCCTATCGCTCATT GGAAAACCTGCCCTTCAGGCTCTTTTATATCACTGCCATTTTTATGAACATTTGAATCAGATGGTAAAG CATTGTTACCTAGGACGGTGTATGTTTGATTTGAATTTTTCTGCTTTTGATAGAAATTCAGAAAGCAAT GATTTAAATGGTTTAGATGACTCATTCAAGTTTTGGAGGGCTCCATCTAGGACAAGTCAGGATCGAGAT CCAAGTTCTCTCTCCACCTCAGAAACAACGGTGGCACCTTCATTGGGGAGTACTGAATTTCAGCCACTT GTGCAGTCAACAACACTTCTACCTGAAGCCTCCCATGACCAGTTTGTGGCTCAAGGTCACCAGGAAAGT ACATCACCACGGCCACCTCATGATTCATCTCTTTCTGCTCCCCTGCCCAAACTGTGTGTTTTTGTTACT CCATCTCTTCTTTCAGCAATGTGCAGCCTCTTGGACAACCTCTTGACGATTGCTCCCAGAGACACTGCA AAGGCTTTTCGACAAGCTCATCTCATAGAACTTCTCTGTAGCATTGCAGATGCTACCCTCATACAGACA TGTGTCCAGGAACTCAGAGCCCTGCTGCCTTCATCACCTCCAGCTGAACACACTCAGGCTCAGGTTTCC TTTCTCCTGGAATACCTATCCTCTTTGTCCAGGCTTCTGCAGTCATGTTTATTGGTGGAGCCTGACCTT GTGATTCAGGATGAGCTTGTGAAACCTCTTATCACCAATATCATTGGAATTCTCACCATATGTACCAAA GATGTATTAGATAAAGAGTTAATATCAGCTTTTTATCACACATGGACACATTTATTTAATCTTCTGGCC ATGCTCCTGAGGAAAGCTGGTGCCATCACACTCCCGTTTGTTACCGTGGCCCTGGCCAAGCACTGGACA GCGGCGATTGATATGTTCTGCACATGTGCAGGCTTGTCTGCCACGTGTCCTGCCCTGTATACTGCCAGC TTGCAATTCCTTTCTGTTCTCTTGACCGAAGAAGCAAAAGGGCATCTCCAGGCTAAGAGCAAAACACAT TTATGCTGTAGTCCAACAGTGGCTTCACTTCTTGATGACTCTCAGGAAAATCAGAAATCTCTAGAACAA CTTAGTGATGTAATCCTTCAGTGCTATGAAGGGAAATCCTCCAAAGATATCCTGAAAAGAGTAGCTGCA AATGCATTGATGTCACTGCTGGCTGTCAGTAGAAGAGCACAGAAACATGCTTTGAAAGCCAATCTTATA GACAATTGCATGGAGCAGATGAAACACATAAATGCACAACTGAACCTAGATTCTCTGAGGCCTGGGAAA GCAGCATTGAAAAAAAAGGAGGATGGTGTTATTAAAGAGTTAAGCATTGCCATGCAGCTCCTAAGAAAC TGTCTTTATCAAAATGAGGAATGTAAAGAAGCAGCTCTTGAAGCTCACCTTGTCCCTGTCTTGCACTCT CTCTGGCCTTGGATTTTGATGGATGATTCATTGATGCAAATTTCTCTGCAGCTCCTTTGTGTCTATACT GCAAATTTTCCAAATGGTTGCAGTTCTCTTTGTTGGTCAAGTTGTGGACAACACCCTGTTCAAGCTACA CATAGAGGAGCCGTGAGCAACTCTCTGATGCTGTGTATCCTAAAGTTGGCTTCCCAGATGCCACTGGAG AACACCACGGTTCAGCAGATGGTTTTTATGCTTCTTTCAAACCTGGCCTTGTCGCATGACTGTAAAGGA GTAATTCAGAAGAGTAACTTCTTACAGAACTTCCTCTCTCTAGCATTGCCAAAAGGAGGAAATAAACAT CTAAGTAATCTGACTATTCTTTGGTTGAAGTTACTCCTGAATATATCATCTGGAGAAGATGGGCAACAA ATGATTCTGAGGCTTGATGGCTGTCTAGACTTACTAACAGAGATGAGCAAATACAAGCACAAGAGCAGC CCTTTATTGCCTCTTCTTATCTTTCATAATGTTTGCTTCAGTCCTGCAAATAAACCCAAGATCCTGGCT AATGAAAAAGTCATTACTGTGCTTGCTGCCTGTCTGGAAAGTGAGAATCAAAATGCTCAGAGGATTGGA GCAGCTGCCCTTTGGGCTCTGATTTACAATTATCAGAAGGCAAAAACAGCTTTGAAAAGCCCATCAGTA AAAAGAAGAGTGGATGAAGCATACTCCTTAGCAAAGAAAACTTTCCCAAACTCAGAAGCAAACCCTCTA AATGCCTATTATTTGAAATGTCTTGAAAACCTCGTGCAGCTCCTTAATTCTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_173630
Insert Size	6681 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_173630.3
RefSeq Size	7109 bp
RefSeq ORF	6681 bp
Locus ID	25914
UniProt ID	Q86VV8
MW	248.6 kDa
Gene Summary	This gene encodes a large protein whose specific function is unknown. Absence of the orthologous protein in mouse results in embryonic lethality with deficient axial rotation, abnormal differentiation of the neural tube, and randomized looping of the heart tube during development. In human, mutations in this gene are associated with polymicrogyria with seizures. In human fibroblasts this protein localizes at the ciliary basal bodies. Given the intracellular localization of this protein and the phenotypic effects of mutations, this gene is suspected of playing a role in the maintenance of normal ciliary structure which in turn effects the developmental process of left-right organ specification, axial rotation, and perhaps notochord development. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.