Gemin 5 (GEMIN5) (NM_015465) Human Untagged Clone
CAT＃: SC309129
GEMIN5 (untagged)-Human gem (nuclear organelle) associated protein 5 (GEMIN5)
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GEMIN-5; NEDCAM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309129 representing NM_015465.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGCAGGAGCCGCGGACGCTGCCGCCCTCCCCCAACTGGTACTGCGCCCGCTGCAGCGATGCCGTG CCCGGGGGCCTCTTTGGCTTCGCCGCGCGGACCTCCGTCTTCCTTGTCCGCGTGGGCCCGGGCGCAGGC GAGAGTCCAGGGACACCCCCGTTTCGAGTCATAGGAGAGTTGGTGGGACACACCGAAAGGGTCTCTGGC TTCACATTTTCTCATCACCCTGGTCAGTACAACCTCTGTGCCACCAGCTCCGACGATGGGACTGTGAAA ATATGGGATGTAGAGACAAAAACAGTTGTGACAGAACATGCACTCCATCAGCATACGATATCAACATTA CATTGGTCTCCTCGAGTAAAGGACTTAATAGTATCTGGGGATGAAAAAGGAGTAGTTTTCTGTTACTGG TTTAACAGAAATGACAGCCAGCACCTCTTTATAGAACCCAGGACAATTTTCTGTCTTACTTGTTCACCT CATCATGAAGATTTAGTAGCCATTGGCTACAAGGATGGCATAGTGGTGATAATTGACATCAGTAAGAAA GGAGAAGTTATTCATAGGCTTCGAGGCCATGATGATGAAATCCACTCCATAGCCTGGTGTCCCCTGCCT GGTGAAGATTGTTTATCTATAAACCAAGAGGAAACTTCAGAAGAAGCTGAAATTACCAACGGGAATGCT GTAGCACAAGCTCCAGTAACAAAAGGTTGCTACTTAGCCACTGGAAGCAAAGATCAAACCATTCGAATC TGGAGCTGTTCTAGAGGCCGAGGGGTGATGATTTTGAAATTGCCCTTTCTGAAGAGAAGAGGAGGGGGT ATAGACCCAACTGTTAAAGAGCGCCTTTGGTTGACACTCCATTGGCCCAGCAATCAACCAACACAGCTG GTATCTAGCTGTTTTGGAGGTGAACTGTTGCAATGGGATCTCACTCAATCTTGGAGACGGAAATACACC CTCTTCAGTGCCTCATCAGAAGGGCAAAATCATTCAAGAATTGTGTTTAATTTATGTCCTTTACAAACA GAGGATGACAAACAGCTATTACTTTCTACATCAATGGATAGAGATGTAAAATGTTGGGACATAGCCACC TTGGAGTGCAGCTGGACCCTTCCTTCCCTTGGTGGGTTTGCATACAGCCTGGCTTTCTCTTCTGTGGAC ATAGGCTCTTTGGCCATAGGTGTTGGGGATGGCATGATCCGTGTATGGAATACACTCTCCATAAAGAAC AACTATGATGTGAAAAATTTTTGGCAAGGCGTGAAGTCCAAGGTTACAGCGCTGTGCTGGCACCCAACC AAGGAAGGTTGCTTAGCTTTTGGAACTGATGATGGAAAAGTGGGATTGTATGACACCTACTCCAACAAG CCTCCACAGATTTCTAGCACATATCATAAGAAGACTGTATATACTTTAGCCTGGGGGCCACCAGTACCC CCCATGTCACTTGGAGGAGAAGGAGACAGACCTTCCCTTGCTTTATACAGCTGTGGAGGAGAAGGGATT GTCTTACAGCATAATCCCTGGAAGCTTAGTGGAGAAGCCTTTGACATCAACAAACTCATCAGGGACACC AATTCAATCAAATACAAATTGCCTGTACACACAGAGATAAGTTGGAAAGCAGATGGCAAAATCATGGCT CTTGGCAATGAAGATGGATCAATAGAAATATTTCAGATTCCCAACCTGAAACTGATCTGTACTATCCAA CAGCATCACAAGCTTGTGAATACCATTAGCTGGCATCATGAGCATGGCAGCCAGCCAGAATTGAGCTAT CTGATGGCCTCTGGCTCCAACAATGCAGTCATTTACGTGCACAACCTGAAGACTGTCATAGAGAGCAGC CCTGAGTCTCCAGTGACCATTACAGAGCCCTACCGGACCCTCTCAGGGCATACGGCCAAGATTACCAGT GTGGCGTGGAGCCCACATCATGATGGAAGGCTGGTATCTGCTTCCTATGATGGTACAGCCCAGGTGTGG GATGCTCTCCGGGAAGAGCCCCTGTGCAATTTCCGAGGACATCGAGGTCGACTGCTTTGTGTGGCATGG TCTCCTTTGGATCCAGACTGCATCTATTCAGGGGCAGATGACTTTTGTGTGCACAAGTGGCTCACTTCC ATGCAAGATCATTCCCGGCCTCCTCAAGGCAAAAAAAGTATTGAATTGGAGAAAAAACGGCTCTCTCAA CCTAAGGCAAAGCCCAAAAAGAAGAAAAAGCCCACCTTGAGAACTCCTGTAAAGCTGGAATCGATTGAT GGAAATGAAGAAGAAAGCATGAAGGAGAACTCAGGACCTGTTGAGAATGGTGTGTCAGACCAAGAAGGG GAGGAGCAAGCACGGGAGCCGGAATTACCCTGTGGCCTTGCTCCAGCGGTTTCTAGAGAACCAGTTATC TGCACTCCAGTTTCCTCAGGCTTTGAAAAGTCAAAAGTCACCATTAATAACAAAGTCATTTTACTGAAA AAGGAGCCACCAAAAGAGAAGCCAGAAACCTTAATCAAGAAGAGAAAAGCTCGTTCCTTGCTTCCCCTG AGTACAAGCCTGGACCACAGATCCAAAGAGGAGCTTCATCAGGACTGTTTGGTACTAGCAACTGCAAAG CACTCCAGAGAGCTGAATGAAGATGTGTCTGCTGATGTTGAGGAAAGATTTCATCTGGGGCTTTTCACA GACAGGGCTACCCTGTATAGAATGATTGATATTGAAGGAAAAGGTCACTTAGAAAATGGCCACCCTGAG TTATTTCACCAGCTTATGCTTTGGAAAGGAGATCTCAAAGGTGTTCTCCAGACTGCAGCAGAAAGAGGG GAGCTGACAGACAACCTTGTGGCTATGGCACCAGCAGCTGGCTACCATGTGTGGCTATGGGCTGTGGAA GCTTTTGCCAAACAGCTGTGTTTTCAGGATCAGTATGTCAAGGCTGCTTCTCACCTACTTTCCATCCAC AAAGTGTATGAAGCGGTGGAGCTGCTCAAGTCAAACCATTTTTACAGGGAAGCTATTGCGATTGCCAAG GCCCGGCTGCGCCCGGAGGACCCAGTCCTGAAGGACTTGTACCTCAGCTGGGGAACCGTCCTAGAAAGA GATGGCCACTATGCTGTAGCTGCCAAATGCTATTTAGGGGCCACTTGTGCTTATGATGCAGCCAAAGTT TTGGCCAAAAAGGGGGATGCGGCATCACTTAGAACGGCTGCAGAGTTGGCTGCCATCGTAGGAGAGGAT GAGTTGTCTGCTTCCCTGGCTCTCAGATGTGCCCAAGAGCTGCTTCTGGCCAACAACTGGGTGGGAGCC CAGGAAGCCCTGCAGCTGCATGAAAGTCTACAGGGTCAGAGATTGGTGTTTTGCCTTCTGGAGCTACTG TCCAGGCATCTGGAGGAAAAGCAGCTTTCAGAGGGCAAAAGCTCCTCCTCTTACCACACTTGGAACACG GGCACCGAAGGGCCTTTCGTGGAGAGGGTGACTGCAGTGTGGAAGAGCATCTTCAGCCTTGACACCCCT GAGCAGTATCAGGAAGCCTTTCAGAAGCTGCAGAACATCAAGTACCCATCTGCTACAAATAACACACCT GCCAAACAGCTCCTGCTTCACATTTGCCATGACTTGACCCTGGCAGTGCTGAGCCAACAGATGGCCTCC TGGGACGAGGCTGTGCAGGCGCTCCTTCGGGCGGTGGTCCGGAGCTATGACTCAGGGAGCTTCACCATC ATGCAGGAAGTGTACTCAGCCTTTCTCCCTGACGGCTGTGACCACCTAAGAGACAAGTTGGGGGACCAT CAATCCCCTGCCACACCAGCTTTCAAAAGTTTGGAGGCCTTTTTTCTTTATGGGCGTCTGTATGAATTC TGGTGGTCTCTCTCCAGACCTTGCCCAAATTCCAGTGTCTGGGTAAGGGCTGGTCACAGAACACTCTCT GTTGAGCCAAGCCAGCAGTTAGACACTGCCAGCACTGAAGAAACGGACCCTGAAACTTCTCAGCCAGAG CCAAACAGGCCTTCAGAACTAGACTTGAGACTCACAGAAGAAGGTGAGCGAATGCTGAGTACTTTTAAG GAGCTCTTTTCAGAAAAGCATGCCAGTCTCCAAAACTCACAGAGAACTGTTGCTGAAGTCCAAGAGACC TTGGCAGAAATGATCCGACAACACCAAAAGAGTCAACTCTGTAAATCCACAGCAAATGGTCCTGATAAG AATGAACCGGAAGTAGAAGCAGAGCAGCCCCTCTGCAGTTCTCAGAGCCAGTGTAAAGAAGAAAAAAAT GAGCCACTTTCTCTGCCTGAGTTAACCAAAAGGCTTACCGAGGCAAATCAGAGAATGGCGAAATTTCCT GAGAGCATTAAGGCCTGGCCCTTCCCAGATGTGCTGGAGTGCTGCCTCGTCCTGCTTCTCATCAGGTCC CACTTTCCTGGCTGTCTGGCCCAGGAAATGCAGCAGCAGGCCCAAGAGCTCCTTCAGAAATACGGCAAC ACGAAAACTTACAGAAGACACTGCCAGACCTTCTGTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_015465 |
| Insert Size | 4527 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015465.4 |
| RefSeq Size | 5404 bp |
| RefSeq ORF | 4527 bp |
| Locus ID | 25929 |
| UniProt ID | Q8TEQ6 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| MW | 168.6 kDa |
| Gene Summary | This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217516 | GEMIN5 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 5 (GEMIN5) |
CNY 10320.00 |
|
| RC217516L3 | Lenti ORF clone of Human gem (nuclear organelle) associated protein 5 (GEMIN5), Myc-DDK-tagged |
CNY 16910.00 |
|
| RC217516L4 | Lenti ORF clone of Human gem (nuclear organelle) associated protein 5 (GEMIN5), mGFP tagged |
CNY 12720.00 |
|
| RG217516 | GEMIN5 (tGFP-tagged) - Human gem (nuclear organelle) associated protein 5 (GEMIN5) |
CNY 16530.00 |
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