RGPD5 (NM_005054) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BS-63; BS63; HEL161; RGP5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309094 representing NM_005054. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGCGCAGCAAGGCCGATGTGGAGCGGTACGTCGCCTCGGTGCTGGGTCTCACCCCGTCGCCTCGA CAGAAGTCAATGAAAGGATTCTATTTTGCAAAGCTGTATTATGAAGCTAAAGAATATGATCTTGCTAAA AAATACATATGTACTTACATTAATGTGCAAGAGAGGGATCCCAAAGCTCACAGATTTCTGGGTCTTCTT TATGAATTGGAAGAAAACACAGAGAAAGCCGTTGAATGTTACAGGCGTTCAGTGGAATTAAACCCAACA CAAAAAGATCTTGTGTTGAAGATTGCAGAATTGCTTTGTAAAAATGATGTTACTGATGGAAGAGCAAAA TACTGGGTCGAAAGAGCAGCAAAACTTTTCCCAGGAAGTCCTGCAATTTATAAACTAAAGGAACAGCTT CTAGATTGTGAAGGTGAAGATGGATGGAATAAACTTTTTGACTTGATTCAGTCAGAACTTTATGTAAGA CCTGATGACGTCCATGTGAACATCCGGCTAGTGGAGTTGTATCGCTCAACTAAAAGATTGAAGGATGCT GTGGCCCACTGCCATGAGGCAGAGAGGAACATAGCTTTGCGTTCAAGTTTAGAGTGGAATTCGTGTGTT GTACAGACCCTTAAGGAATATCTGGAGTCTTTACAGTGTTTGGAGTCTGATAAAAGTGACTGGCAAGCA ACCAATACAGACTTACTGCTGGCCTATGCTAATCTTATGCTTCTTACGCTTTCCACTAGAGATGTGCAG GAAAATAGAGAATTACTGGAAAGTTTTGATAGTGCTCTTCAGTCTGCGAAATCTTCTTTGGGTGGAAAT GATGAACTGTCAGCTACTTTCTTAGAAATGAAAGGACATTTCTATATGTATGCTGGTTCTCTGCTCTTG AAGATGGGTCAGCATGGTAATAATGTTCAATGGCGAGCTCTTTCTGAGCTGGCTGCATTGTGCTATCTC ATAGCATTTCAGGTTCCAAGACCAAAGATTAAATTAAGAGAAGGTAAAGCTGGACAAAATCTGCTGGAA ATGATGGCCTGTGACCGACTGAGCCAATCAGGGCACATGTTGCTAAGCTTAAGTCGTGGCAAGCAAGAT TTCTTAAAAGAGGTTGTTGAAACTTTTGCCAACAAAATTGGGCAGTCTGCGTTATATGATGCTCTGTTT TCTAGTCAGTCACCTAAGGATACATCTTTTCTTGGTAGCGATGATATTGGAAAAATTGATGTACAAGAA CCAGAGCTTGAAGATTTGGCTAGATACGATGTTGGTGCTATTCGAGCACATAATGGTAGTCTTCAGCAT CTTACTTGGCTTGGCTTACAGTGGAATTCATTGCCTGCTTTACCTGGAATCCGAAAATGGCTAAAACAG CTTTTCCATCGTTTGCCCCATGAAACCTCAAGGCTTGAAACAAATGCGCCTGAATCAATATGTATTTTA GATCTTGAAGTATTTCTCCTTGGAGTAGTATATACCAGCCACTTACAATTAAAGGAGAAATGTAATTCT CACCATAGCTCCTATCAGCCGTTATGCCTGCCCTTTCCTGTGTGTAAACAGCTTTGTACAGAAAGACAA AAATCTTGGTGGGATGCGGTTTGTACTCTGATTCACAGAAAAGCAGTACCTGGAAACTTGGCAAAATTG AGACTTCTAGTTCAGCATGAAATAAACACTCTAAGAGCCCAGGAAAAACATGGCCTTCAACCTGCTCTG CTTGTACATTGGGCAAAATACCTTCAGAAAACGGGCAGCGGTCTTAATTCTTTTTATGGTCAACTAGAA TACATAGGGAGAAGTGTTCATTATTGGAAGAAAGTTTTGCCATTGTTGAAGATAATAAAGAAGAACAGT ATTCCTGAACCTATTGATCCTCTGTTTAAACATTTTCATAGTGTAGACATTCAGGCATCAGAAATTGTT GAATATGAAGAAGACGCACACATAACTTTTGCTATGTTGGATGCAGTAAATGGAAATATAGAAGATGCT GTGACTGCTTTTGAATCTATAAAAAGTGTTGTTTCTTATTGGAATCTTGCACTGATTTTTCACAGGAAG GCAGAAGACATTGAAAATGATGCCCTTTCTCCTGAAGAACAAGAAGAATGCAGAAATTATCTGACAAAG ACCAGGGACTACCTAATAAAGATTATAGATGACGGTGATTCAAATCTTTCAGTGGTCAAGAAATTGCCT GTGCCCCTGGAGTCTGTAAAACAGATGCTTAATTCAGTCATGCAGGAACTCGAAGACTATAGTGAAGGA GGTCCTCTCTATAAAAATGGTTCTTTGCGAAATGCAGATTCAGAAATAAAACATTCTACACCGTCTCCT ACCAAATATTCACTATCACCAAGTAAAAGTTACAAGTATTCTCCCGAAACACCACCTCGATGGACAGAA GATCGGAATTCTTTACTGAATATGATTTGCCAACAAGTAGAGGCCATTAAGAAAGAAATGCAGGAGTTG AAACTAAATAGCAGTAAGTCAGCATCCCGTCATCGTTGGCCCACAGAGAATTATGGACCAGACTCGGTG CCTGATGGATATCAGGGGTCACAGACATTTCATGGGGCTCCACTAACAGTTGCAACTACTGGCCCTTCA GTATATTATAGTCAGTCACCAGCATATAATTCCCAGTATCTTCTCAGACCAGCAGCTAATGTTACTCCC ACAAAGGGTTCTTCTAATACAGAATTTAAGTCAACCAAAGAAGGATTTTCCATCCCTGTGTCTGCTGAT GGATTTAAATTTGGCATTTCGGAACCAGGAAATCAAGAAAAGAAAAGGGAAAAGCCTCTTGAAAATGAT ACTGGCTTCCAGGCTCAGGATATTAGTGGCCGGAAGAAGGGCCGTGGTGTGATTTTTGGCCAAACAAGT AGCACTTTTACATTTGCAGATGTTGCAAAATCAACTTCAGGAGAAGGATTTCAGTTTGGCAAAAAAGAC CTCAATTTCAAGGGATTTTCAGGTGCTGGAGAAAAATTATTCTCATCACGATACGGTAAAATGGCCAAT AAAGCAAACACTTCCGGTGACTTTGAGAAAGATGATGATGCCTATAAGACTGAGGACAGCGATGACATC CATTTTGAACCAGTAGTTCAAATGCCTGAAAAAGTAGAACTTGTAACAGGAGAAGAAGGTGAAAAAGTT CTGTATTCACAGGGGGTAAAACTATTTAGATTTGATGCTGAGGTAAGGCAGTGGAAAGAAAGGGGCTTG GGGAACTTAAAAATTCTCAAAAACGAGGTCAATGGCAAACTAAGAATGCTGATGCGAAGAGAACAAGTA CTAAAAGTGTGTGCTAATCATTGGATAACGACTACAATGAACCTGAAGCCCCTCTCTGGATCAGATAGA GCATGGATGTGGTCAGCCAGTGATTTCTCTGACGGTGATGCCAAACTAGAGCGGTTGGCAGCAAAATTT AAAACACCAGAGCTGGCTGAAGAATTCAAGCAGAAATTTGAGGAATGCCAGCGGCTTCTGTTAGACATA CCACTTCAAACTCCCCATAAACTTGTAGATACTGGCAGAGCTGCCAAGTTAATACAGAGAGCTGAAGAA ATGAAGAGTGGACTGAAAGATTTCAAAACATTTTTGACAAATGATCAAACAAAAGTCACTGAGGAAGAA AATAAGGGTTCAGGTACAGGTGCGGCCGGTGCCTCAGACACAACAATAAAACCCAATGCTGAAAACACT GGGCCCACATTAGAATGGGATAACTATGACTTAAGGGAAGATGCTTTGGATGATAGTGTCAGTAGTAGC TCAGTACATGCTTCTCCATTGGCAAGTAGCCCTGTGAGAAAAAATCTTTTCCGCTTTGATGAGTCAACA ACAGGATCTAACTTCAGTTTTAAATCTGCTTTGAGTCTATCTAAGTCTCCTGCCAAGTTGAATCAGAGT GGGACTTCAGTTGGCACTGATGAAGAATCTGTTGTTACTCAAGAAGAAGAGAGAGATGGACAGTACTTT GAACCTGTTGTTCCTTTACCTGATCTAGTTGAAGTATCCAGTGGTGAGGAAAATGAACAAGTTGTTTTT AGTCACAGGGCAGAAATCTACAGATATGATAAAGATGTTGGTCAATGGAAAGAAAGGGGCATTGGTGAT ATAAAGATTTTACAGAATTATGATAATAAGCAAGTTCGTATAGTGATGAGAAGGGACCAAGTATTAAAA CTTTGTGCCAATCACAGAATAACTCCAGACATGAGTTTGCAAAATATGAAAGGGACAGAAAGAGTATGG GTGTGGACTGCATGTGATTTTGCAGATGGAGAAAGAAAAGTAGAGCATTTAGCTGTTCGTTTTAAACTA CAGGATGTTGCAGACTCGTTTAAGAAAATTTTTGATGAAGCAAAAACAGCCCAGGAAAAAGATTCTTTG ATAACACCTCATGTTTCTCGGTCAAGCACTCCCAGAGAGTCACCATGTGGCAAAATTGCTGTAGCTATA TTAGAAGAAACCACAAGAGAGAGGACAGATGTTATTCAGGGTGATGATGTAGCAGATGCAGCTTCAGAA GTTGAAGTGTCTAGCACATCTGAAACAACAACAAAAGCAGTGGTTTCTCCTCCAAAGTTTGTATTTGTT TCAGAGTCTGTTAAAAGAATTTTTAGTAGTGAAAAATCAAAACCATTTGTATTTGGCAACAGTTCTGCC ACTGGGTCTTTGTTTGGATTTAGTTTTAATGCACCTTTGAAAAGTAACAATAGTGAAACTAGTTCAGTA GCCCAGAGTGGATCTGAAAGCAAAGTGGAACCTAAAAAATGTGAACTGTCAAAGAACTCTGATATCGAA CAGTCTTCAGATAGCAAAGTCAAAAATCTCTCTGCTTCCTTTCCAACGGAAGAATCTTCAATCAACTAC ACATTTAAAACACCAGAAAAGGAGCCTCCATTATGGCATGCTGAATTTACCAAAGAAGAATTGGTTCAG AAGCTCCGTTCCACCACAAAAAGTGCAGATCACTTAAACGGCCTGCTTCGGGAAATAGAGGCAACCAAT GCAGTCCTTATGGAGCAAATTAAGCTTCTCAAAAGTGAAATAAGAAGATTGGAAAGGAATCAAGAGCGA GAGAAGTCTGCAGCTAACCTGGAATACTTGAAGAACGTCTTGCTGCAGTTCATTTTCTTGAAGCCAGGT AGTGAAAGAGAGAGACTTCTTCCTGTTATAAATACGATGTTGCAGCTCAGCCCTGAAGAAAAGGGAAAA CTTGCTGCGGTTGCTCAAGATGAGGAAGAAAATGCTTCCCGTTCTTCTGGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_005054
Insert Size	5298 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_005054.2
RefSeq Size	7182 bp
RefSeq ORF	5298 bp
Locus ID	84220
UniProt ID	Q99666
Protein Families	Druggable Genome
MW	198.9 kDa
Gene Summary	RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene shares a high degree of sequence identity with RANBP2, a large RAN-binding protein localized at the cytoplasmic side of the nuclear pore complex. It is believed that this RANBP2 gene family member arose from a duplication event 3 Mb distal to RANBP2. Alternative splicing has been observed for this locus and two variants are described. Additional splicing is suggested but complete sequence for further transcripts has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the longest transcript encoding the largest isoform (1).

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