GBF1 (NM_004193) Human Untagged Clone
CAT＃: SC308995
GBF1 (untagged)-Human golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1
CNY 35820.00
货期*
5周
规格
Product images
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARF1GEF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF Sequence for NM_004193. The custom clone sequence may differ by one or more nucleotides
ATGGTGGATAAGAATATTTACATCATTCAAGGGGAGATTAACATTGTGGTTGGGGCCATC AAACGAAATGCCCGATGGAGCACCCATACACCACTGGATGAAGAACGGGATCCTCTGCTG CATAGTTTCGGTCATCTAAAGGAGGTTTTAAACAGTATAACAGAACTCTCAGAAATTGAG CCCAATGTATTCCTTCGACCTTTTCTGGAAGTGATTCGCTCTGAAGATACCACTGGCCCT ATCACTGGACTGGCACTCACCTCTGTCAACAAGTTCCTGTCCTATGCACTCATAGATCCC ACCCATGAGGGCACAGCAGAGGGCATGGAGAACATGGCAGATGCTGTCACCCATGCTCGT TTTGTGGGCACGGATCCTGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAGGTTCTA CGGACTCTGCTGCTAACCCCAGTGGGTGCCCACCTAACCAATGAATCTGTGTGTGAGATT ATGCAGTCTTGCTTCCGGATCTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCC GCAGAGCACACTCTCGTAGACATGGTGCAGCTGCTCTTCACAAGGTTACCTCAGTTTAAA GAAGAACCCAAGAACTATGTGGGGACCAACATGAAGAAGCTGAAAATGAGAGCCGGAGGC ATGAGTGATTCATCCAAATGGAAGAAACAGAAGAGATCCCCTCGGCCCCCACGCCATATG ACCAAAGTCACACCAGGTTCAGAGCTGCCCACTCCCAATGGAACCACCTTATCATCTAAC CTCACTGGTGGCATGCCCTTCATTGATGTGCCCACTCCCATCTCCTCTGCAAGTTCAGAA GCTGCCTCAGCAGTGGTCAGTCCCTCTACAGACAGTGGCCTGGAATTCTCCTCCCAAACC ACTTCCAAGGAAGACCTTACTGATCTAGAGCAACCTGGCTCTCCAGGGTACAGCACAGCT ACAGAGCCTGGAAGCAGTGAGCTAGGTGTTCCCGAGCAGCCTGACCTCCAGGAAGGGACC CATGTGGAAAAGTCCCAGTCAGCATCTGTGGAGTCCATCCCTGAAGTGTTAGAGGAGTGC ACGTCCCCTGCCGACCACTCTGACTCTGCCTCTGTCCATGACATGGATTACGTCAATCCC CGGGGCGTGCGCTTTACACAGTCCTCCCAGAAAGAAGGCACAGCTTTGGTCCCCTATGGT CTTCCCTGCATCCGCGAGCTCTTCCGCTTCCTCATCTCCCTCACCAATCCACACGACCGC CATAACTCAGAGGTTATGATTCACATGGGACTGCATTTGCTGACAGTGGCCCTTGAGTCA GCCCCTGTAGCCCAGTGCCAGACCCTCTTGGGCCTCATCAAGGATGAGATGTGCCGTCAC TTATTCCAGCTACTCAGCATAGAGCGACTAAACCTTTATGCTGCTTCCCTGCGAGTATGC TTCCTACTGTTTGAGAGCATGCGAGAGCACCTCAAGTTCCAAATGGAGATGTACATCAAA AAGCTTATGGAGATCATCACTGTGGAGAACCCCAAGATGCCTTATGAGATGAAGGAGATG GCACTGGAGGCCATTGTGCAGCTCTGGCGCATCCCCAGCTTTGTCACAGAGCTCTACATC AACTATGATTGTGACTACTACTGTTCCAACCTCTTTGAGGAACTCACAAAGCTGCTGTCC AAGAATGCCTTCCCTGTGTCTGGTCAACTCTATACAACACACCTACTATCTCTTGATGCC CTATTGACAGTGATTGACAGCACCGAGGCCCACTGCCAGGCTAAAGTCCTCAACAGCCTC ACCCAGCAAGAGAAGAAGGAGACAGCCAGACCAAGCTGTGAGATAGTAGATGGCACCCGA GAAGCTAGCAATACTGAGAGAACTGCCAGCGATGGGAAAGCTGTAGGCATGGCCTCAGAC ATCCCAGGCCTGCATCTGCCAGGTGGAGGGCGGCTGCCACCAGAACATGGGAAATCAGGA TGCAGTGATCTGGAGGAAGCTGTTGACTCTGGGGCTGACAAAAAGTTTGCCCGGAAGCCA CCCCGATTTTCCTGTCTCCTGCCAGATCCACGGGAACTAATTGAAATTAAAAACAAAAAG AAGCTGCTAATCACTGGCACAGAGCAGTTCAATCAGAAACCAAAGAAGGGGATTCAGTTT CTGCAAGAGAAAGGCCTCCTCACCATCCCAATGGACAACACAGAGGTTGCTCAGTGGCTC CGAGAGAACCCTCGGCTGGACAAGAAGATGATTGGAGAGTTTGTGAGTGACCGCAAAAAC ATTGACCTGTTGGAGAGCTTTGTGAGCACCTTCAGTTTTCAGGGTCTGCGACTGGACGAA GCCCTCCGCCTCTACCTGGAAGCCTTCCGTTTGCCTGGGGAAGCACCAGTCATCCAGAGG TTGCTGGAGGCATTCACAGAGCGTTGGATGAATTGTAATGGCTCCCCATTTGCCAATAGC GATGCCTGCTTTTCCCTGGCCTATGCTGTCATCATGCTTAATACTGACCAGCACAACCAC AATGTTCGTAAACAGAATGCACCCATGACCCTGGAGGAGTTTCGCAAAAATCTGAAAGGT GTGAATGGAGGCAAGGACTTTGAGCAAGACATCCTGGAGGACATGTACCATGCCATCAAG AATGAGGAAATTGTAATGCCTGAGGAGCAGACAGGCTTGGTTCGGGAGAACTATGTGTGG AATGTGCTGCTTCATCGAGGTGCCACCCCTGAGGGCATATTCCTGCGTGTGCCTACTGCC AGCTATGATCTTGACCTCTTCACCATGACCTGGGGCCCCACTATTGCTGCTCTCTCTTAT GTCTTTGACAAAAGCCTTGAGGAGACAATCATCCAGAAAGCCATCTCAGGCTTCAGGAAG TGCGCCATGATCTCCGCCCACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCT CTATGCAAATTCACAGCTCTCAGCAGTGAGTCTATTGAGAACCTGCCCAGTGTATTTGGA AGCAACCCTAAAGCCCATATTGCAGCCAAGACAGTATTCCATTTGGCCCATCGTCATGGT GACATCCTGCGGGAGGGCTGGAAGAATATCATGGAGGCCATGCTGCAGCTCTTCCGAGCC CAACTACTGCCCAAGGCTATGATAGAGGTAGAAGATTTCGTGGATCCCAATGGCAAGATC TCTCTACAGCGGGAAGAGACACCATCAAACCGAGGAGAGTCAACAGTGCTGAGCTTTGTG AGCTGGCTAACACTGAGTGGTCCTGAGCAGTCTAGTGTTCGGGGCCCATCCACTGAAAAC CAAGAGGCCAAGAGAGTGGCCTTAGAGTGTATAAAGCAATGTGACCCAGAAAAAATGATC ACAGAAAGCAAGTTCCTCCAGCTGGAGTCACTACAGGAGCTCATGAAGGCTCTGGTCTCA GTGACACCAGATGAAGAGACATATGATGAGGAAGATGCTGCTTTCTGCCTAGAGATGCTG CTAAGGATTGTGTTGGAGAACAGGGATCGTGTGGGCTGTGTGTGGCAGACTGTTCGAGAC CATCTATACCACCTCTGTGTTCAGGCACAAGATTTCTGCTTCCTTGTGGAGCGGGCAGTG GTGGGGTTGCTACGCCTGGCCATTCGGCTTCTCCGGAGAGAAGAGATCAGTGCTCAGGTG CTGCTCTCCCTGCGCATTTTGCTACTGATGAAGCCCAGTGTGCTATCCCGAGTCAGCCAC CAGGTTGCGTATGGGCTCCATGAACTCCTGAAGACCAATGCAGCCAACATCCACTCAGGT GATGACTGGGCCACACTCTTCACACTGCTGGAGTGCATCGGCTCAGGTGTGAAGCCTCCA GCTGCTCTGCAGGCCACAGCCAGGGCAGATGCACCTGATGCCGGGGCCCAGTCAGATAGT GAGCTCCCATCCTACCATCAGAATGACGTGAGCCTGGATCGAGGGTACACTTCCGACTCA GAGGTCTACACTGACCATGGCAGGCCGGGCAAGATACACCGATCAGCCACAGATGCCGAT GTGGTCAACAGTGGTTGGTTAGTGGTTGGGAAGGATGACGTTGATAACTCCAAGCCAGGG CCCAGCCGCCCAGGCCCTTCACCCCTGATCAATCAATACAGCCTAACAGTGGGACTGGAT TTGGGGCCACACGACACTAAGTCTCTGCTTAAGTGTGTGGAATCGCTGTCCTTCATCGTG CGTGATGCTGCCCACATCACACCTGACAACTTTGAGCTCTGCGTCAAGACTCTCCGGATC TTTGTGGAGGCCAGTCTGAATGGCGGATGCAAGTCCCAGGAGAAACGTGGCAAGAGTCAC AAATATGACAGCAAAGGGAACCGCTTCAAGAAGAAATCCAAAGAGGGATCAATGCTTCGC CGGCCTCGAACCTCCAGCCAACATGCCTCTCGGGGCGGGCAGAGTGATGATGATGAGGAC GAAGGCGTGCCTGCCAGCTACCATACGGTGTCTTTACAGGTCAGTCAGGACTTGCTAGAC CTGATGCACACCCTGCACACGCGGGCAGCCTCTATCTACAGCTCATGGGCGGAGGAGCAA CGCCACCTGGAGACAGGTGGCCAGAAGATTGAAGCTGATTCTCGCACCCTCTGGGCCCAC TGCTGGTGCCCTTTACTGCAGGGTATTGCCTGCCTGTGCTGCGATGCCCGGCGCCAGGTA CGGATGCAGGCACTGACCTATCTGCAGCGAGCACTACTTGTACATGATCTGCAAAAGCTA GATGCCCTGGAATGGGAGTCCTGTTTTAACAAGGTGCTGTTTCCTCTACTTACCAAGCTC TTGGAGAACATCAGCCCTGCAGATGTGGGTGGGATGGAGGAGACCCGGATGAGGGCTTCC ACATTGCTCTCTAAGGTCTTCCTGCAGCACCTGTCTCCACTGCTGTCACTCTCTACCTTT GCGGCCCTCTGGCTCACCATCTTGGACTTCATGGACAAGTACATGCACGCAGGCTCCAGC GACTTACTGTCAGAGGCGATCCCTGAGTCTCTGAAGAACATGCTTCTGGTGATGGACACA GCGGAGATTTTCCACAGTGCAGATGCACGGGGAGGCGGCCCCTCGGCCCTCTGGGAGATC ACCTGGGAACGCATTGACTGTTTTCTCCCTCACCTACGAGATGAACTCTTCAAGCAGACC GTCATCCAGGACCCCATGCCCATGGAGCCTCAAGGCCAAAAGCCTCTCGCCTCAGCCCAC CTGACTTCCGCTGCTGGCGACACTAGGACACCTGGCCATCCACCGCCCCCAGAGATTCCA TCTGAGCTGGGGGCCTGTGACTTTGAGAAGCCCGAGAGCCCCCGAGCCGCCAGCAGCAGC TCCCCAGGATCACCAGTGGCCTCAAGCCCCAGCAGGCTGAGCCCCACCCCCGACGGGCCT CCACCCTTGGCTCAGCCCCCACTGATCCTGCAGCCCTTGGCCTCCCCACTGCAGGTGGGC GTGCCACCTATGACTCTGCCCATCATCCTCAACCCTGCGCTCATCGAGGCCACCTCACCA GTGCCCCTCCTGGCCACACCCCGCCCCACAGATCCCATACCCACCTCTGAGGTCAAC |
| Restriction Sites | Please inquire |
| ACCN | NM_004193 |
| Insert Size | 6400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004193.1, NP_004184.1 |
| RefSeq Size | 6376 bp |
| RefSeq ORF | 5580 bp |
| Locus ID | 8729 |
| UniProt ID | Q92538 |
| Domains | Sec7 |
| Gene Summary | This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220987 | GBF1 (Myc-DDK-tagged)-Human golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1 |
CNY 12496.00 |
|
| RG220987 | GBF1 (tGFP-tagged) - Human golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant 1 |
CNY 20330.00 |
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