Myosin IXA (MYO9A) (NM_006901) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMS24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308958 representing NM_006901. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATATAAATGATGGAGGAAGACGACGCTTTGAAGATAATGAACATACATTACGGATATATCCTGGG GCTATTTCAGAAGGGACAATCTACTGTCCGATTCCTGCCAGAAAAAACTCCACAGCTGCTGAGGTGATT GAGTCTCTTATAAACAAACTTCATCTTGACAAAACAAAATGTTATGTTCTAGCAGAGGTAAAGGAATTT GGTGGAGAAGAATGGATTCTCAATCCAACAGATTGTCCAGTTCAGCGAATGATGCTGTGGCCCCGAATG GCTCTGGAAAATCGCTTAAGTGGAGAGGACTACCGCTTCCTTCTGAGAGAGAAAAACCTTGATGGATCA ATCCATTATGGTAGCCTGCAGTCATGGCTACGGGTAACAGAAGAACGTCGCAGGATGATGGAACGGGGT TTTCTTCCACAGCCTCAACAGAAAGACTTTGATGATTTATGTAGTTTACCTGATTTGAATGAGAAAACT CTCTTAGAAAACCTACGAAATCGCTTTAAGCATGAAAAAATTTATACCTATGTTGGCAGTATTCTAATA GTTATTAACCCATTCAAGTTTCTTCCTATTTATAACCCCAAATATGTCAAAATGTATGATAACCACCAA CTGGGAAAACTTGAGCCCCACATTTATGCTGTGGCTGATGTAGCTTATCATGCCATGCTTCAGCGCAAA AAGAATCAGTGCATCGTGATTTCAGGAGAGAGTGGTTCTGGGAAGACTCAAAGCACAAACTTTCTTATT CACCACCTTACTGCTCTCAGTCAGAAAGGATTTGCCAGTGGAGTAGAACAGATTATTCTTGGAGCTGGA CCAGTACTTGAGGCCTTTGGAAATGCAAAGACAGCTCATAATAACAATTCAAGTCGTTTTGGGAAGTTT ATTCAAGTAAATTACCAGGAAACAGGCACTGTACTTGGTGCCTATGTTGAAAAATATCTACTGGAGAAG TCCAGACTCGTTTATCAGGAGCATAATGAACGGAACTATCATGTATTCTATTACCTCCTGGCAGGAGCA AGTGAAGATGAGAGATCAGCATTCCATCTTAAGCAACCAGAGGAATATCATTATCTCAATCAGATAACA AAGAAACCCCTCAGACAGAGCTGGGATGATTATTGCTATGACTCTGAGCCGGATTGCTTCACGGTGGAA GGAGAAGATTTGAGACATGACTTTGAGCGCCTACAACTTGCCATGGAAATGGTAGGATTTCTTCCCAAG ACACGAAGACAGATTTTCTCTCTTCTCTCAGCCATACTACATTTGGGTAATATCTGTTACAAAAAGAAG ACATACCGGGATGACTCCATTGATATCTGTAATCCTGAAGTTCTGCCTATTGTCTCAGAATTATTAGAG GTTAAAGAAGAGATGCTATTTGAAGCATTAGTTACAAGGAAGACGGTGACAGTGGGAGAAAAGCTTATT TTGCCATACAAGTTGGCAGAGGCTGTGACAGTGAGGAACTCCATGGCTAAGTCTCTGTATAGTGCCCTG TTTGACTGGATAGTTTTTCGAATTAATCATGCACTTCTGAATAGTAAAGATTTAGAGCATAATACCAAG ACATTGTCTATTGGTGTTCTTGATATTTTTGGGTTTGAAGATTATGAAAATAACAGCTTTGAACAGTTC TGTATTAATTTTGCTAATGAACGTTTACAGCACTACTTTAATCAGCATATCTTTAAATTGGAACAAGAG GAATATAGAACTGAAGGTATCAGCTGGCACAACATAGATTACATTGATAATACCTGCTGCATAAATCTT ATTAGCAAAAAACCAACAGGACTGCTTCATCTTTTGGATGAAGAAAGCAACTTTCCACAGGCTACAAAT CAAACATTGCTAGACAAGTTTAAGCATCAACATGAAGATAATTCTTACATCGAATTTCCAGCCGTGATG GAGCCTGCTTTCATTATAAAACATTATGCTGGAAAAGTAAAATATGGGGTAAAGGATTTCCGGGAAAAA AATACAGATCATATGCGCCCAGACATTGTAGCTCTTCTGAGAAGCAGCAAGAATGCATTTATCTCTGGG ATGATTGGAATTGATCCTGTAGCTGTTTTCCGATGGGCAATTCTCCGAGCTTTTTTCAGAGCCATGGTT GCTTTCAGGGAAGCTGGGAAAAGAAACATTCACAGAAAAACTGGACATGATGATACAGCGCCATGTGCA ATTTTGAAAAGTATGGATAGTTTTAGCTTTCTCCAACACCCAGTCCACCAGAGGAGCTTAGAGATTCTG CAGAGATGCAAGGAAGAGAAGTACAGTATAACCCGGAAAAATCCCAGAACACCTCTTTCTGATCTCCAG GGCATGAATGCTCTAAATGAAAAAAACCAACATGATACATTTGATATTGCCTGGAATGGCAGAACTGGG ATTCGCCAGAGCAGACTATCAAGTGGCACCTCCTTGCTTGATAAAGATGGAATATTTGCTAATTCAACT AGCAGCAAACTCCTGGAGAGAGCCCATGGAATTCTCACGAGAAACAAAAATTTCAAATCCAAGCCTGCC CTTCCAAAGCACTTGCTAGAAGTAAATTCTTTAAAGCACCTGACAAGACTGACACTACAAGATCGCATT ACCAAGTCTCTTCTTCATTTACACAAGAAGAAAAAACCTCCCAGCATCAGTGCCCAGTTTCAGGCATCA TTAAGCAAGCTAATGGAAACACTTGGTCAAGCAGAACCATATTTTGTAAAATGCATTCGCTCTAATGCT GAAAAGCTGCCCTTAAGGTTCAGTGATGTCTTGGTACTTAGACAGCTTCGATACACCGGGATGCTGGAA ACAGTTCGAATTCGCCAATCAGGATACAGCTCCAAATATTCTTTCCAGGATTTTGTGAGCCACTTCCAT GTACTTCTTCCCCGAAATATTATTCCATCCAAATTTAACATTCAGGATTTCTTCAGGAAAATAAATCTT AATCCAGATAATTATCAAGTTGGAAAAACCATGGTCTTTCTAAAGGAGCAGGAACGACAGCACTTACAA GATCTGCTTCACCAAGAGGTGCTCCGCAGAATCATATTGTTGCAGCGATGGTTCAGGGTCTTGCTGTGT AGGCAGCATTTCCTCCATCTGAGACAAGCATCTGTTATTATCCAGAGATTCTGGAGGAATTACCTAAAT CAGAAGCAAGTCAGAGATGCAGCTGTGCAGAAGGATGCTTTTGTTATGGCTAGTGCAGCTGCTCTTCTC CAAGCTTCCTGGCGTGCTCACTTAGAGAGGCAGCGGTACTTGGAGTTACGGGCTGCAGCCATCGTTATC CAGCAGAAATGGAGAGATTACTATAGGCGCAGGCACATGGCTGCTATTTGCATACAAGCAAGATGGAAA GCCTACAGGGAAAGTAAAAGGTACCAAGAACAAAGGAAAAAAATTATCCTTTTGCAATCAACATGTAGA GGATTCAGAGCAAGACAAAGATTTAAAGCTTTAAAAGAACAAAGGCTAAGAGAAACAAAGCCAGAAGTT GGATTGGTGAATATTAAGGGATATGGATCTCTGGAAATTCAGGGTTCAGACCCTTCAGGATGGGAGGAT TGTTCTTTTGACAACAGAATAAAAGCCATAGAGGAATGTAAATCTGTAATAGAGAGTAATCGAATTAGC CGTGAAAGTTCAGTGGACTGCTTGAAGGAGTCACCAAACAAGCAGCAGGAGAGAGCCCAAAGCCAGAGT GGTGTGGACTTGCAGGAAGATGTGCTTGTAAGAGAGAGACCCAGATCCTTGGAGGATCTCCATCAGAAA AAAGTAGGCCGGGCTAAGAGAGAAAGTAGGAGAATGAGAGAACTAGAGCAAGCTATATTTAGCTTAGAA TTGCTGAAAGTTCGTTCTCTTGGTGGTATTTCTCCTTCAGAGGATCGCAGATGGTCTACAGAATTGGTG CCTGAAGGCCTTCAGTCTCCACGGGGTACACCTGATAGTGAGAGCTCTCAAGGAAGCTTGGAACTTCTG AGCTATGAGGAAAGCCAAAAGAGCAAACTAGAGTCTGTCATTTCAGATGAAGGAGACTTGCAGTTTCCA TCACCTAAGATATCCAGCAGTCCAAAATTTGATTCACGGGACAATGCCCTCAGTGCCTCAAATGAGACT AGCAGTGCAGAGCATTTGAAGGATGGAACTATGAAGGAAATGGTGGTCTGCAGTTCTGAGTCTATTACC TGTAAACCACAGCTGAAAGACTCCTTCATTTCAAATAGTCTACCTACTTTTTTTTATATCCCCCAACAA GACCCACTGAAAACAAATTCCCAACTAGACACAAGTATCCAAAGAAACAAACTATTGGAAAATGAAGAC ACAGCGGGGGAAGCTCTTACTTTGGATATCAACAGGGAAACTAGAAGGTATCACTGCTCAGGAAAAGAT CAGATTGTTCCTTCTTTGAATACAGAGTCTTCTAATCCTGTGCTTAAGAAGTTAGAAAAGCTAAACACT GAGAAGGAAGAAAGGCAAAAACAGTTGCAGCAACAGAATGAAAAAGAGATGATGGAACAGATTCGCCAG CAAACAGATATTTTAGAGAAGGAGCGCAAAGCCTTCAAGACAATTGAAAAGCCAAGAATTGGAGAGTGT TTGGTGGCACCATCTTCCTATCAGTCAAAGCAAAGAGTAGAGAGGCCATCCTCTCTCCTCAGCTTAAAT ACCTCAAATAAGGGAGAACTTAATGTACTGGGGTCCCTATCATTAAAAGATGCAGCTCTTGCCCAAAAA GACAGTTCCTCTGCTCACTTACCCCCAAAGGACCGACCTGTCACCGTGTTCTTTGAAAGAAAAGGAAGT CCATGCCAATCTAGTACTGTCAAGGAATTATCCAAGACAGACAGAATGGGCACCCAGCTGAATGTAGCC TGTAAACTCTCAAATAATCGCATTTCAAAAAGAGAACACTTTAGGCCAACTCAGTCTTACAGCCACAAT TCTGATGACCTTTCCAGAGAGGGAAATGCTAGGCCCATTTTCTTCACTCCAAAGGACAATATGAGTATT CCCCTTGTCAGCAAAGAAGCCTTAAACAGTAAAAATCCTCAACTCCATAAAGAAGATGAACCAGCATGG AAACCTGTGAAGTTAGCTGGGCCAGGCCAAAGAGAGACATCACAGCGATTTTCGTCAGTTGATGAACAA GCAAAACTTCATAAGACTATGTCTCAAGGAGAGATTACCAAGTTGGCAGTGAGACAGAAGGCTTCAGAT TCAGATATAAGACCTCAGAGAGCTAAGATGAGATTCTGGGCCAAAGGGAAACAAGGGGAGAAGAAGACT ACCAGAGTGAAACCTACTACCCAGTCAGAGGTTTCGCCACTCTTTGCAGGCACAGATGTGATTCCAGCT CATCAGTTTCCAGATGAATTAGCTGCATATCACCCAACACCTCCTTTGAGCCCAGAACTGCCCGGCAGT TGCCGGAAGGAATTCAAAGAGAACAAAGAACCTTCTCCAAAGGCTAAGCGCAAGCGAAGTGTGAAGATT AGCAACGTGGCTTTGGATTCTATGCATTGGCAAAATGACTCTGTCCAGATCATAGCAAGTGTCAGTGAT TTAAAAAGCATGGATGAATTTCTTCTGAAAAAGGTGAATGACCTAGATAATGAAGACAGCAAGAAGGAT ACACTAGTGGATGTTGTATTTAAAAAAGCCCTGAAGGAATTTCGGCAGAATATCTTCAGCTTTTATTCA TCTGCATTGGCGATGGATGATGGGAAAAGCATACGGTATAAAGACCTCTATGCACTATTTGAACAGATT CTGGAAAAGACGATGAGGCTTGAGCAGCGTGATTCACTGGGTGAATCTCCAGTGAGAGTTTGGGTCAAC ACTTTTAAAGTGTTTTTAGATGAATATATGAATGAATTCAAGACTTCAGATTGCACAGCCACAAAGGTG CCAAAAACAGAAAGAAAGAAAAGAAGGAAAAAGGAAACTGATTTGGTGGAAGAACACAATGGTCACATC TTTAAAGCCACCCAATATAGCATCCCTACATACTGTGAATACTGTTCTTCTTTGATATGGATAATGGAC CGAGCCTCTGTTTGCAAATTATGCAAGTATGCTTGCCATAAGAAGTGCTGTCTGAAAACCACAGCCAAG TGCTCTAAAAAGTATGATCCAGAGCTGTCATCTCGACAATTTGGGGTTGAACTGTCCCGTTTGACCAGT GAAGACCGAACTGTTCCTTTAGTAGTGGAAAAGCTCATAAACTACATTGAAATGCATGGACTGTATACA GAAGGTATTTATCGAAAGTCTGGTTCGACTAATAAAATCAAGGAGCTTCGGCAGGGTCTAGATACAGAT GCTGAGAGTGTAAATCTAGATGACTATAACATACACGTCATTGCAAGTGTATTCAAACAATGGCTTCGA GATTTGCCCAATCCTCTCATGACCTTTGAACTCTATGAGGAATTTCTTCGAGCTATGGGCCTTCAGGAG AGGAAGGAGACAATCCGTGGTGTATACTCTGTGATTGATCAACTCTCCCGAACTCATCTCAATACACTG GAACGCCTCATCTTTCATCTAGTCAGGATTGCTCTGCAGGAAGACACTAATCGAATGTCTGCTAATGCT TTGGCCATTGTGTTTGCGCCCTGCATTCTCCGCTGCCCTGACACCACTGACCCACTACAAAGTGTACAG GACATCAGTAAGACTACCACTTGTGTGGAACTGATTGTTGTGGAACAAATGAATAAATACAAGGCTCGT CTCAAAGATATCAGTAGCTTGGAATTTGCTGAGAATAAGGCAAAGACCAGGTTGTCACTGATTCGTAGA TCAATGGGAAAGGGGCGTATTCGTCGAGGAAACTATCCAGGTCCATCGTCTCCTGTTGTAGTTCGGTTG CCTTCTGTGTCTGATGTCTCAGAGGAGACCTTGACTAGTGAGGCAGCCATGGAGACTGACATCACAGAA CAGCAGCAAGCAGCTATGCAGCAGGAGGAGAGAGTACTGACTGAGCAGATTGAGAACCTACAGAAGGAG AAGGAGGAGCTAACATTTGAGATGCTTGTACTGGAACCCCGTGCCTCTGATGATGAAACCCTTGAGTCT GAGGCCTCCATTGGGACTGCTGATAGCTCAGAGAATTTGAATATGGAGTCTGAATATGCTATCTCTGAG AAATCAGAAAGAAGCTTAGCCCTTAGCTCCCTGAAGACAGCTGGCAAGTCTGAACCTTCCAGCAAGTTG CGAAAGCAACTTAAAAAGCAGCAAGACTCTTTAGATGTCGTGGACTCTTCGGTCTCCTCTTTATGTCTG TCTAACACGGCATCATCTCATGGGACCAGAAAACTATTTCAGATTTATTCCAAATCTCCATTCTACCGA GCTGCCTCAGGTAATGAGGCCCTGGGAATGGAAGGACCATTGGGCCAGACCAAATTCCTGGAAGACAAG CCTCAGTTCATCAGCAGAGGAACCTTCAACCCGGAAAAGGGCAAACAAAAATTAAAGAATGTGAAAAAC TCACCTCAGAAAACCAAAGAGACCCCAGAGGGGACAGTCATGTCTGGCCGCAGAAAAACTGTGGACCCA GACTGCACCTCCAACCAACAGCTAGCACTCTTTGGAAATAATGAATTTATGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_006901
Insert Size	7647 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006901.3
RefSeq Size	8722 bp
RefSeq ORF	7647 bp
Locus ID	4649
UniProt ID	B2RTY4
Domains	IQ, RA, RhoGAP, myosin_head, DAG_PE-bind
MW	292.7 kDa
Gene Summary	This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. They function as actin-based molecular motors. Mutations in this gene have been associated with Bardet-Biedl Syndrome. [provided by RefSeq, Dec 2011]

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