KIF13A (NM_022113) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA500C11.2; RBKIN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308822 representing NM_022113. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGATACCAAGGTAAAAGTTGCCGTCCGGGTCCGGCCCATGAACCGACGAGAACTGGAACTGAAC ACCAAGTGCGTGGTGGAGATGGAAGGGAATCAAACGGTCCTGCACCCTCCTCCTTCTAACACCAAACAG GGAGAAAGGAAACCTCCCAAGGTATTTGCCTTTGATTATTGCTTTTGGTCCATGGATGAATCTAACACT ACAAAATACGCTGGTCAAGAAGTGGTTTTCAAGTGCCTTGGGGAAGGAATTCTTGAAAAAGCCTTTCAG GGGTATAATGCGTGTATTTTTGCATATGGACAGACAGGTTCGGGAAAATCCTTTTCCATGATGGGCCAT GCTGAGCAGCTGGGCCTTATTCCAAGGCTCTGCTGTGCTTTATTTAAAAGGATCTCTTTGGAGCAAAAT GAGTCACAGACCTTTAAAGTTGAAGTGTCCTATATGGAAATTTATAATGAGAAAGTTCGGGATCTTTTA GACCCCAAAGGGAGTAGACAGTCTCTTAAAGTTCGAGAACATAAAGTTTTGGGACCATATGTAGATGGT TTATCTCAACTAGCTGTCACTAGTTTTGAGGATATTGAGTCATTGATGTCTGAGGGAAATAAGTCTCGA ACGGTAGCTGCTACCAACATGAACGAAGAAAGCAGCCGCTCCCATGCTGTGTTCAACATCATAATCACA CAGACACTTTATGACCTGCAGTCTGGGAATTCCGGGGAGAAAGTCAGTAAGGTCAGCTTGGTAGACCTG GCGGGTAGCGAAAGAGTATCTAAAACAGGAGCTGCAGGAGAGCGACTGAAAGAAGGCAGCAACATTAAC AAATCGCTTACAACCTTGGGGTTGGTTATATCATCACTGGCTGACCAGGCAGCTGGCAAGGGTAAAAGC AAATTTGTGCCTTATCGAGATTCAGTCCTCACTTGGCTGCTTAAGGACAACTTGGGGGGCAACAGCCAA ACCTCTATGATAGCCACAATCAGCCCAGCCGCAGACAACTATGAAGAGACCCTCTCCACATTAAGATAT GCAGACCGAGCCAAAAGGATTGTGAACCATGCTGTTGTGAATGAGGACCCCAACGCAAAAGTGATCCGA GAACTGCGGGAGGAAGTCGAGAAACTGAGAGAGCAGCTCTCTCAGGCAGAGGCCATGAAGGCCCCTGAA CTGAAGGAGAAGCTCGAAGAGTCTGAAAAGCTGATAAAAGAACTAACAGTGACTTGGGAAGAGAAGCTG AGAAAAACAGAAGAGATAGCACAGGAAAGACAACGACAACTTGAAAGCATGGGGATTTCCCTGGAGATG TCCGGTATCAAGGTGGGGGATGACAAATGCTACTTAGTCAATCTGAATGCAGACCCTGCTCTTAACGAA CTTCTGGTTTATTATTTAAAGGATCACACCAGGGTGGGTGCAGATACCTCTCAAGATATCCAGCTTTTT GGCATAGGAATTCAGCCTCAGCACTGTGAGATTGACATTGCATCTGATGGAGACGTCACTCTCACTCCA AAAGAAAATGCAAGGTCCTGTGTGAACGGCACCCTTGTGTGCAGTACCACCCAGCTGTGGCATGGTGAC CGAATCCTATGGGGAAATAATCACTTTTTTAGAATAAACTTACCTAAGAGGAAACGTCGAGATTGGTTG AAAGACTTTGAAAAAGAAACGGGCCCGCCAGAGCATGACCTGGATGCAGCCAGTGAGGCTTCCTCTGAA CCAGACTATAACTATGAATTTGCACAGATGGAAGTTATCATGAAAACCCTGAATAGTAATGACCCAGTT CAAAATGTGGTTCAGGTCCTGGAGAAACAATACCTAGAAGAAAAGAGAAGTGCCCTAGAGGAGCAGCGG CTCATGTATGAGCGGGAACTGGAGCAACTCCGCCAGCAGCTCTCCCCCGACAGGCAGCCACAGAGTAGC GGCCCTGACCGCCTGGCCTACAGCAGCCAGACAGCGCAGCAGAAGGTGACCCAGTGGGCAGAAGAGAGG GATGAACTCTTCCGACAAAGCCTGGCAAAACTGCGAGAGCAGCTGGTTAAAGCTAATACCTTGGTGAGG GAAGCAAACTTCCTGGCTGAGGAAATGAGCAAACTCACCGATTACCAAGTGACTCTTCAGATCCCTGCT GCAAACCTCAGTGCCAATAGGAAGAGAGGTGCAATAGTGAGTGAACCAGCTATCCAAGTGAGGAGGAAA GGAAAGAGCACCCAAGTGTGGACCATTGAGAAGCTGGAGAATAAATTAATTGACATGAGAGACCTTTAC CAAGAATGGAAGGAAAAAGTTCCTGAGGCAAAGAGACTCTACGGAAAACGAGGTGACCCTTTCTATGAA GCCCAAGAAAATCACAACCTCATCGGGGTGGCGAATGTATTCTTGGAATGCCTCTTCTGTGATGTGAAA CTTCAGTATGCAGTCCCTATCATCAGCCAGCAGGGGGAGGTTGCAGGGCGTCTCCACGTGGAAGTGATG CGTGTTACAGGAGCTGTTCCAGAGCGTGTGGTGGAGGATGACTCTTCGGAGAATTCCAGTGAAAGTGGG AGCCTTGAAGTCGTAGACAGCAGCGGGGAAATCATTCACCGAGTCAAAAAGCTGACATGTCGGGTAAAA ATTAAAGAAGCAACGGGGCTGCCCTTAAACCTCTCAAATTTTGTCTTCTGTCAATACACATTCTGGGAC CAGTGTGAGTCTACGGTGGCTGCCCCGGTGGTGGACCCCGAGGTGCCTTCACCACAGTCCAAGGATGCC CAGTACACAGTGACCTTCTCCCACTGTAAGGACTATGTGGTGAATGTAACAGAAGAATTTCTGGAGTTC ATTTCAGATGGAGCACTGGCCATTGAAGTATGGGGCCACCGGTGTGCTGGAAATGGCAGCTCCATCTGG GAGGTCGATTCTCTTCATGCTAAGACAAGAACACTGCATGACAGGTGGAATGAAGTAACGCGAAGAATA GAAATGTGGATCTCCATATTAGAATTGAATGAGTTAGGAGAGTATGCTGCAGTGGAACTTCATCAGGCA AAAGATGTCAACACAGGAGGCATCTTTCAACTTAGACAGGGTCATTCCCGTAGAGTACAAGTCACGGTG AAACCTGTGCAGCATTCAGGGACACTGCCACTTATGGTTGAAGCCATCCTGTCAGTATCCATCGGCTGT GTAACTGCCAGGTCCACCAAACTCCAAAGAGGGCTGGACAGTTACCAGAGAGATGATGAGGATGGTGAT GATATGGATAGTTATCAGGAAGAAGACTTAAACTGCGTAAGGGAGAGGTGGTCAGATGCACTCATTAAA CGACGAGAATACCTGGATGAACAGATAAAAAAAGTCAGCAATAAAACAGAGAAAACAGAGGACGATGTG GAGCGGGAAGCCCAGCTTGTGGAGCAGTGGGTAGGGCTGACTGAGGAAAGGAATGCTGTGCTGGTGCCA GCCCCAGGCAGTGGGATTCCTGGGGCACCTGCCGACTGGATCCCACCTCCTGGAATGGAAACCCACATA CCAGTTCTCTTCCTCGATTTGAATGCGGATGACCTCAGTGCCAATGAGCAGCTTGTTGGCCCCCATGCA TCCGGCGTGAACTCCATCCTGCCCAAGGAGCATGGCAGCCAGTTTTTCTACCTGCCCATCATAAAGCAC AGTGATGATGAGGTTTCAGCCACAGCCTCTTGGGATTCCTCGGTGCATGATTCTGTTCACTTGAATAGG GTCACACCACAGAATGAAAGGATTTACCTAATTGTGAAAACCACAGTTCAACTCAGCCACCCTGCTGCT ATGGAGTTAGTATTACGAAAACGAATTGCAGCCAATATTTACAACAAACAGAGTTTCACGCAGAGTTTG AAGAGGAGAATATCCCTGAAAAATATATTTTATTCCTGTGGTGTAACCTATGAAATAGTATCCAATATA CCAAAGGCAACTGAGGAGATAGAGGACCGGGAAACGCTGGCTCTCCTGGCAGCAAGGAGTGAAAACGAA GGCACATCAGATGGGGAGACGTACATTGAGAAGTACACTCGAGGCGTGCTGCAGGTGGAAAACATTCTG AGTCTTGAACGGCTCCGGCAGGCCGTCACAGTCAAAGAAGCACTTTCCACCAAAGCCCGGCACATTCGG AGGAGCCTCAGTACACCAAATGTTCATAATGTCTCTTCCAGCCGACCGGACCTTTCTGGCTTTGATGAA GATGACAAGGGTTGGCCAGAGAACCAGTTGGACATGTCTGACTATAGCTCCAGTTACCAAGATGTAGCA TGTTATGGAACTTTACCCAGGGATTCTCCTCGAAGGAATAAAGAAGGTTGTACATCAGAGACTCCTCAT GCCTTAACCGTCAGCCCTTTTAAAGCATTCTCTCCTCAGCCACCAAAGTTTTTCAAGCCCCTAATGCCT GTAAAAGAGGAGCATAAGAAAAGGATAGCCCTGGAAGCAAGGCCTCTTCTAAGCCAGGAGAGCATGCCT CCACCTCAGGCACATAACCCTGGCTGCATTGTACCCTCAGGAAGCAATGGCAGCAGCATGCCAGTAGAA CACAATAGCAAACGTGAGAAGAAGATTGACTCTGAGGAGGAAGAAAATGAGCTGGAAGCTATTAACAGG AAGCTAATAAGTTCACAGCCTTATGTACCTGTGGAGTTTGCTGACTTCAGTGTTTACAATGCCAGCTTG GAGAACAGGGAATGGTTTTCCTCTAAAGTAGATCTGTCAAACTCACGGGTCTTGGAGAAAGAAGTGTCC CGTAGCCCTACCACCAGCAGTATTACCAGTGGCTACTTTTCCCACAGTGCCTCCAATGCCACCCTGTCT GACATGGTGGTCCCTTCTAGTGACAGCTCAGACCAGCTGGCCATTCAGACGAAGGATGCAGACTCCACC GAGCACTCCACACCATCGCTTGTGCATGATTTCAGGCCGTCCTCAAACAAAGAGTTGACAGAAGTCGAA AAAGGCTTGGTAAAGGACAAGATAATTGTGGTGCCACTCAAGGAAAACAGTGCCTTAGCCAAAGGGAGC CCATCATCCCAGAGCATCCCTGAGAAAAACTCCAAATCACTGTGCAGGACTGGCTCATGTTCAGAACTA GATGCCTGCCCCAGCAAAATTAGCCAGCCAGCCAGGGGATTCTGCCCCAGGGAGGTGACGGTAGAACAC ACCACCAACATCCTTGAAGACCATTCTTTCACAGAATTTATGGGAGTGTCAGAGGGAAAAGATTTTGAT GGTTTGACAGATTCTTCTGCTGGAGAGCTTTCCAGTAGGAGGAGTCTACCAAATAAAACAGGCGGCAAG ACTGTCTCCGATGGGCTCCACCACCCCAGCCAGCTGCATTCCAAGTTAGAGAATGACCAGGTAATAATT CCAGAGGCAGCCTTTTGGGTTCTGTGCTGTCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_022113
Insert Size	5418 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022113.5
RefSeq Size	6005 bp
RefSeq ORF	5418 bp
Locus ID	63971
UniProt ID	Q9H1H9
Domains	kinesin
Protein Families	Druggable Genome
MW	202.3 kDa
Gene Summary	This gene encodes a member of the kinesin family of microtubule-based motor proteins that function in the positioning of endosomes. This family member can direct mannose-6-phosphate receptor-containing vesicles from the trans-Golgi network to the plasma membrane, and it is necessary for the steady-state distribution of late endosomes/lysosomes. It is also required for the translocation of FYVE-CENT and TTC19 from the centrosome to the midbody during cytokinesis, and it plays a role in melanosome maturation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) encodes the longest isoform (a, also known as RBKIN1).

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