ATP6V1H (NM_213620) Human Untagged Clone
CAT＃: SC308683
ATP6V1H (untagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 3
CNY 7220.00
Product images
CNY 6281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-11; MSTP042; NBP1; SFD; SFDalpha; SFDbeta; VMA13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308683 representing NM_213620.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCAAAATGGATATCCGAGGTGCTGTGGATGCTGCTGTCCCCACCAATATTATTGCTGCCAAGGCT GCAGAAGTTCGTGCAAACAAAGTCAACTGGCAATCCTATCTTCAGGGACAGATGATTTCTGCTGAAGAT TGTGAGTTTATTCAGAGGTTTGAAATGAAACGAAGCCCTGAAGAGAAGCAAGAGATGCTTCAAACTGAA GGCAGCCAGTGTGCTAAAACATTTATAAATCTGATGACTCATATCTGCAAAGAACAGACCGTTCAGTAT ATACTAACTATGGTGGATGATATGCTGCAGGAAAATCATCAGCGTGTTAGCATTTTCTTTGACTATGCA AGATGTAGCAAGAACACTGCGTGGCCCTACTTTCTGCCAATGTTGAATCGCCAGGATCCCTTCACTGTT CATATGGCAGCAAGAATTATTGCCAAGTTAGCAGCTTGGGGAAAAGAACTGATGGAAGGCAGTGACTTA AATTACTATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTGCGTGGTAGCGGTGTTGCTGTT GAAACAGGAACAGTCTCTTCAAGTGATAGTTCGCAGTATGTGCAGTGCGTGGCCGGGTGTTTGCAGCTG ATGCTCCGGGTCAATGAGTACCGCTTTGCTTGGGTGGAAGCAGATGGGGTAAATTGCATAATGGGAGTG TTGAGTAACAAGTGTGGCTTTCAGCTCCAGTATCAAATGATTTTTTCAATATGGCTCCTGGCATTCAGT CCTCAAATGTGTGAACACCTGCGGCGCTATAATATCATTCCAGTTCTGTCTGATATCCTTCAGGAGTCT GTCAAAGAGAAAGTAACAAGAATCATTCTTGCAGCATTTCGTAACTTTTTAGAAAAATCAACTGAAAGA GAAACTCGCCAAGAATATGCCCTGGCTATGATTCAGTGCAAAGTTCTGAAACAGTTGGAGAACTTGGAA CAGCAGAAGTACGATGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAAAAACTTGGAGAGAGT GTCCAGGACCTTAGTTCATTTGATGAATACAGTTCAGAACTTAAATCTGGAAGGTTGGAATGGAGTCCT GTGCACAAATCTGAGAAATTTTGGAGAGAGAATGCTGTGAGGTTAAATGAGAAGAATTATGAACTCTTG AAAATCTTGACAAAACTTTTGGAAGTGTCAGATGATCCCCAAGTCTTAGCTGTTGCTGCTCACGATGTT GGAGAATATGTGCGGCATTATCCACGAGGCAAACGGGTCATCGAGCAGCTCGGTGGGAAGCAGCTGGTC ATGAACCACATGCATCATGAAGACCAGCAGGTCCGCTATAATGCTCTGCTGGCCGTGCAGAAGCTCATG GTGCACAACTGGGAATACCTTGGCAAGCAGCTCCAGTCCGAGCAGCCCCAGACCGCTGCCGCCCGAAGC TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_213620 |
| Insert Size | 1452 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_213620.2 |
| RefSeq Size | 2080 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 51606 |
| UniProt ID | Q9UI12 |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| MW | 55.9 kDa |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217790 | ATP6V1H (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 3 |
CNY 3656.00 |
|
| RC217790L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 3, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC217790L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 3, mGFP tagged |
CNY 5890.00 |
|
| RG217790 | ATP6V1H (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 3 |
CNY 4370.00 |
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