FREM2 (NM_207361) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CRYPTOP; FRASRS2
Vector	PCMV6-Neo
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308479 representing NM_207361. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCACTCAGCCGGGACTCCCGGGTTATCCTCGCGCCGGACAGGCAACTCCACCAGCTTTCAACCAGGA CCGCCACCGCCGCCCCGGCTGCTGCTGCTGCTGCTGCTTCTCCTGTCACTGGTAAGCCGCGTCCCGGCA CAGCCCGCTGCCTTCGGCAGGGCGTTGCTGTCCCCTGGTCTCGCGGGGGCTGCAGGGGTCCCTGCTGAG GAGGCCATAGTGCTGGCGAACCGCGGACTCCGGGTGCCTTTCGGCCGTGAAGTCTGGCTGGATCCCCTG CATGACCTGGTGTTGCAGGTGCAGCCCGGGGACCGCTGCGCGGTTTCGGTACTAGACAACGACGCACTG GCCCAGCGACCGGGCCGCCTGAGTCCCAAGCGCTTCCCGTGCGACTTTGGCCCTGGCGAGGTGCGCTAC TCTCACCTGGGCGCGCGCAGCCCGTCTCGGGACCGCGTCCGGCTGCAGCTGCGCTATGACGCGCCCGGA GGGGCAGTAGTGCTACCACTGGTACTGGAGGTGGAGGTGGTCTTCACCCAGCTGGAGGTTGTGACTCGG AACTTGCCTCTGGTCGTGGAAGAACTGCTGGGGACCAGCAATGCCCTGGACGCGCGGAGCCTGGAGTTC GCCTTCCAGCCCGAGACAGAGGAGTGCCGCGTGGGCATCCTGTCCGGCTTGGGCGCGCTGCCTCGCTAT GGAGAACTCCTCCACTACCCGCAGGTCCCTGGAGGAGCCAGAGAGGGAGGCGCCCCGGAGACTCTCCTG ATGGACTGCAAAGCTTTCCAGGAACTAGGCGTGCGCTATCGCCACACAGCCGCCAGTCGCTCACCAAAC AGGGACTGGATACCCATGGTGGTGGAGCTGCGTTCACGAGGGGCTCCTGTGGGCAGCCCTGCTTTGAAA CGCGAGCACTTCCAGGTTCTGGTGAGGATCCGAGGAGGGGCCGAGAACACTGCACCCAAGCCCAGTTTC GTGGCCATGATGATGATGGAGGTGGACCAGTTTGTACTGACGGCCCTGACCCCAGACATGCTGGCAGCC GAGGATGCTGAGTCTCCCTCTGACCTGTTGATCTTCAACCTTACTTCTCCATTCCAGCCTGGCCAGGGC TACTTGGTGAGCACCGATGATCGCAGCCTGCCCCTTTCCTCCTTCACTCAGAGGGATCTGCGGCTCCTG AAGATTGCCTACCAGCCCCCTTCTGAAGACTCTGACCAGGAGCGCCTCTTTGAACTGGAATTGGAGGTA GTGGATCTAGAAGGAGCAGCTTCAGACCCTTTTGCCTTCATGGTAGTGGTGAAGCCCATGAACACAATG GCTCCGGTGGTCACCCGGAATACCGGTCTTATTCTCTATGAGGGTCAGTCTCGGCCCCTCACAGGCCCT GCAGGCAGTGGTCCGCAAAACTTGGTCATCAGCGATGAGGATGACCTAGAAGCAGTGCGGCTAGAGGTG GTGGCTGGGCTCCGGCATGGTCACCTTGTCATTCTGGGTGCTTCCAGTGGCAGCTCTGCTCCCAAGAGC TTTACAGTGGCTGAGCTGGCAGCTGGCCAGGTGGTCTACCAGCATGATGACAGAGACGGCTCGCTGAGC GACAACCTGGTGCTTCGCATGGTGGATGGAGGAGGCAGGCACCAGGTACAGTTTCTGTTCCCCATCACC TTAGTGCCTGTGGATGACCAGCCACCTGTTCTCAATGCCAACACGGGGCTGACACTGGCAGAGGGTGAA ACAGTGCCCATCCTGCCCCTTTCCCTGAGTGCAACTGACATGGATTCAGATGATTCTCTGCTGCTTTTT GTGCTGGAGTCACCCTTCTTAACTACGGGGCATCTGCTTCTCCGCCAAACTCACCCTCCCCATGAGAAG CAGGAACTTCTCAGAGGCCTTTGGAGGAAGGAGGGGGCATTTTATGAGCGAACAGTGACAGAGTGGCAG CAGCAGGACATAACAGAGGGCAGGCTGTTCTATAGACACTCTGGGCCCCATAGTCCTGGGCCAGTCACA GACCAGTTCACATTTAGAGTCCAGGATAACCATGACCCTCCTAATCAGTCCGGGCTACAGCGGTTTGTG ATTCGTATCCATCCTGTGGATCGCCTCCCTCCGGAGCTGGGCAGTGGCTGTCCCCTTCGTATGGTGGTA CAGGAATCCCAGCTCACACCACTGAGGAAGAAGTGGCTGCGCTACACTGACCTGGACACAGATGACCGA GAACTACGTTACACAGTGACTCAGCCCCCCACAGACACAGACGAAAATCACCTGCCAGCCCCACTGGGT ACCTTGGTCTTGACTGACAACCCCTCAGTCGTGGTGACCCATTTTACCCAAGCCCAGATCAACCATCAT AAAATTGCTTACAGACCCCCGGGTCAAGAACTGGGCGTGGCTACTCGAGTGGCCCAGTTCCAGTTCCAG GTGGAAGACCGAGCTGGGAATGTGGCTCCAGGTACCTTTACCCTTTACTTGCATCCCGTGGACAACCAG CCACCTGAGATCCTCAACACCGGCTTCACTATTCAGGAGAAGGGTCACCACATCCTGAGTGAGACAGAG TTGCACGTGAATGATGTAGACACTGATGTTGCCCATATCTCTTTCACTCTCACTCAGGCACCCAAACAT GGCCACATGAGAGTGTCTGGACAGATCCTGCATGTAGGGGGTCTCTTCCACTTGGAGGACATAAAACAG GGCCGAGTTTCCTATGCCCATAATGGGGACAAGTCCCTGACTGATAGCTGCTCCTTGGAAGTCAGTGAC AGACATCATGTGGTGCCCATCACTCTCAGAGTAAATGTCCGGCCAGTGGATGATGAAGTGCCCATACTG AGCCATCCTACTGGCACTCTGGAGTCCTATCTAGATGTCTTAGAAAATGGGGCTACTGAAATCACTGCC AATGTTATTAAGGGGACCAATGAGGAAACTGATGACTTGATGTTGACTTTCCTCTTGGAAGATCCACCT TTGTATGGGGAAATCTTGGTCAATGGCATTCCAGCAGAGCAGTTTACTCAAAGGGACATCTTGGAGGGC TCTGTTGTATATACCCACACCAGTGGTGAGATAGGCCTATTGCCTAAAGCGGATTCTTTTAACCTGAGT CTGTCAGATATGTCTCAAGAATGGAGAATTGGTGGCAATACTATCCAAGGAGTTACTATATGGGTGACC ATCCTGCCTGTTGATAGCCAGGCCCCAGAAATCTTTGTAGGTGAACAGTTGATAGTAATGGAAGGTGAT AAAAGTGTTATAACATCAGTGCATATAAGTGCTGAAGATGTCGACTCCCTGAATGATGACATCTTGTGC ACTATAGTTATTCAGCCTACTTCAGGTTATGTTGAAAACATTTCTCCAGCACCAGGCTCTGAGAAATCA AGAGCAGGGATTGCCATAAGTGCTTTCAACTTGAAAGATCTCAGGCAGGGCCACATAAACTATGTCCAG AGTGTCCATAAAGGGGTGGAACCTGTGGAGGACCGATTTGTATTTCGTTGTTCTGATGGCATTAACTTT TCAGAGAGACAGTTCTTCCCCATTGTAATCATTCCCACCAATGATGAACAGCCAGAGATGTTTATGAGA GAATTTATGGTGATGGAAGGCATGAGTCTGGTAATTGATACACCCATTCTCAATGCTGCTGATGCTGAT GTTCCCCTGGATGATTTAACTTTCACTATTACCCAATTCCCCACTCATGGTCACATCATGAATCAGCTG ATAAATGGCACGGTTTTGGTCGAAAGCTTCACCTTGGATCAGATCATAGAGAGTTCCAGCATTATTTAT GAGCATGATGACTCCGAGACCCAGGAAGACAGTTTTGTGATTAAACTAACAGATGGGAAGCACTCTGTG GAAAAGACGGTCCTCATTATAGTTATCCCTGTTGATGATGAGACGCCCAGAATGACTATCAATAATGGA CTAGAAATAGAAATTGGGGATACCAAGATTATCAACAACAAAATATTAATGGCAACAGATTTAGATTCA GAAGACAAATCTTTGGTTTATATTATTCGTTATGGGCCAGGACATGGCTTATTACAGAGACGAAAACCT ACTGGTGCCTTTGAAAATATCACACTGGGCATGAATTTTACCCAGGATGAAGTAGACAGAAACTTAATT CAGTATGTCCATTTGGGGCAAGAGGGCATTCGGGACCTAATTAAATTTGATGTGACTGATGGAATAAAT CCCCTCATAGATCGTTACTTTTATGTGTCCATCGGGAGCATTGACATTGTCTTCCCTGATGTGATAAGT AAGGGAGTGTCCTTGAAAGAAGGTGGCAAAGTCACTCTTACAACAGACCTACTAAGCACTAGTGACTTG AACAGTCCTGATGAAAACTTGGTTTTTACCATCACCAGGGCTCCCATGCGAGGTCACCTGGAATGCACG GATCAGCCTGGTGTGTCCATCACGTCTTTCACTCAGCTGCAACTGGCTGGAAACAAAATCTACTACATC CACACAGCTGATGATGAAGTGAAAATGGACAGTTTTGAGTTTCAAGTCACCGATGGACGTAACCCTGTC TTTCGGACATTCCGTATCTCCATTAGCGATGTGGACAATAAAAAGCCAGTGGTCACCATCCACAAGCTG GTTGTCAGTGAAAGTGAAAACAAGCTGATTACTCCTTTTGAGCTCACTGTCGAAGACAGAGATACTCCT GACAAGCTCCTGAAATTCACTATCACCCAGGTGCCTATTCATGGCCATCTCCTATTCAACAATACCAGA CCTGTCATGGTTTTTACCAAGCAAGACTTGAATGAAAACTTAATCAGCTACAAACATGATGGCACTGAG TCAAGTGAAGATAGCTTCTCCTTCACAGTGACTGATGGCACCCATACAGACTTCTATGTTTTTCCTGAT ACGGTGTTTGAAACAAGGAGACCCCAAGTGATGAAGATCCAGGTCTTGGCTGTTGACAACAGTGTCCCC CAAATCGCAGTGAATAAGGGGGCCTCTACACTTCGCACTCTAGCCACTGGCCACTTGGGGTTCATGATC ACAAGCAAAATATTGAAAGTGGAGGACAGAGACAGCTTACACATTTCTCTTAGATTTATCGTGACAGAG GCCCCTCAACATGGATATCTTCTCAACCTGGACAAAGGCAACCACAGCATCACTCAGTTCACACAAGCT GACATTGATGACATGAAAATATGCTATGTCTTAAGAGAAGGGGCTAATGCCACAAGTGATATGTTCTAT TTTGCAGTTGAAGATGGTGGTGGAAACAAGTTAACGTACCAGAATTTTCGTCTGAATTGGGCATGGATC TCCTTTGAAAAGGAATATTACCTGGTCAATGAGGACTCCAAATTTCTAGATGTTGTTCTTAAACGTAGA GGTTACTTGGGAGAAACTTCTTTTATAAGTATTGGCACAAGAGACAGAACTGCAGAAAAAGACAAAGAC TTCAAGGGCAAAGCACAGAAACAAGTGCAGTTCAACCCAGGCCAGACCAGGGCCACATGGCGAGTGCGG ATCCTGAGTGATGGGGAGCATGAGCAGTCTGAAACCTTTCAGGTGGTACTCTCAGAGCCCGTGCTGGCT GCCTTGGAATTCCCCACAGTCGCCACTGTTGAGATCGTTGATCCAGGAGATGAGCCAACTGTGTTTATT CCCCAGTCCAAATACTCCGTTGAAGAAGATGTTGGTGAGCTGTTCATTCCCATCAGGAGGAGCGGAGAT GTGAGCCAGGAGTTGATGGTGGTCTGTTATACCCAACAAGGAACAGCAACTGGAACTGTGCCGACTTCC GTGTTGTCTTACTCTGATTACATATCCAGGCCTGAGGACCACACCAGTGTTGTCCGCTTTGACAAAGAT GAACGGGAGAAACTGTGTCGGATAGTCATAATTGATGACTCTTTGTACGAGGAGGAGGAAACCTTCCAT GTCCTTCTGAGCATGCCCATGGGGGGAAGAATCGGATCAGAGTTCCCAGGGGCTCAAGTTACAATCGTT CCTGACAAAGATGATGAACCCATCTTTTACTTCGGTGATGTGGAATACTCTGTGGATGAGAGTGCTGGC TATGTGGAAGTGCAGGTGTGGAGAACGGGCACTGACCTGTCCAAGTCTTCTAGTGTCACAGTGAGGTCT CGGAAAACAGATCCTCCCTCTGCAGATGCTGGAACAGACTATGTGGGCATCAGCCGTAATTTAGATTTT GCACCTGGAGTCAACATGCAGCCTGTTCGTGTTGTCATTCTGGATGACCTTGGACAACCAGCGCTGGAG GGAATTGAGAAATTTGAACTGGTGCTTCGCATGCCTATGAACGCAGCCCTTGGCGAGCCCAGCAAAGCC ACAGTGTCCATAAATGACTCTGTCTCCGATTTGCCTAAGATGCAATTCAAAGAACGAATATATACTGGC AGCGAAAGTGATGGGCAGATAGTTACAATGATCCATAGGACTGGGGATGTCCAGTACAGATCTTCAGTG AGATGCTACACCCGGCAGGGGTCTGCACAGGTGATGATGGACTTTGAAGAACGCCCAAACACTGATACC TCCATCATCACATTCCTCCCTGGTGAGACAGAAAAGCCCTGCATTCTTGAGCTGATGGACGATGTGCTC TATGAGGAGGTAGAGGAGCTCCGCCTGGTACTCGGCACTCCACAAAGCAACTCTCCCTTTGGGGCTGCA GTTGGTGAACAAAATGAAACTCTCATAAGGATCCGAGATGATGCTGATAAGACTGTTATTAAATTTGGA GAAACCAAATTTAGTGTCACTGAACCCAAAGAACCTGGAGAGTCGGTGGTTATAAGAATTCCAGTGATT CGCCAAGGAGACACTTCAAAGGTTTCCATTGTGAGAGTCCACACCAAGGATGGCTCGGCCACCTCTGGA GAAGACTACCACCCTGTGTCAGAAGAAATTGAGTTTAAGGAAGGGGAAACCCAGCACGTGGTTGAAATC GAAGTTACCTTTGACGGGGTGAGAGAGATGAGAGAGGCCTTCACTGTTCACCTAAAACCTGATGAAAAT ATGATAGCAGAGATGCAGTTGACGAAAGCCATTGTGTACATAGAAGAAATGAGCAGCATGGCAGATGTC ACTTTTCCTTCTGTCCCTCAAATTGTATCCCTGTTGATGTATGACGACACTTCCAAAGCTAAGGAGAGT GCTGAACCCATGTCTGGCTATCCTGTCATCTGTATCACAGCTTGCAACCCCAAATATTCAGACTACGAT AAAACAGGCTCTATCTGTGCAAGTGAGAACATCAATGACACTTTGACGCGGTACCGGTGGCTGATTAGT GCACCTGCGGGCCCTGACGGTGTGACCAGCCCTATGAGAGAAGTGGACTTCGACACCTTTTTTACGTCA TCCAAGATGGTCACACTGGACTCCATATACTTTCAGCCTGGCTCCCGGGTACAGTGCGCAGCTCGTGCT GTGAACACCAATGGGGATGAAGGCCTGGAGCTCATGAGCCCTATTGTAACCATCAGCAGAGAAGAAGGT CTTTGTCAGCCCCGTGTACCTGGGGTTGTTGGAGCAGAGCCGTTCTCAGCTAAATTGCGCTACACAGGC CCTGAGGATGCAGACTACACAAACCTTATCAAGCTCACTGTCACAATGCCACACATAGATGGCATGCTC CCCGTGATCTCCACTAGAGAGCTTTCCAACTTTGAGCTCACCCTCAGCCCTGATGGCACAAGAGTTGGA AACCACAAGTGCTCCAACCTCCTGGATTATACTGAAGTGAAGACTCATTATGGTTTCTTGACTGATGCT ACCAAAAATCCAGAAATAATTGGAGAGACATATCCTTACCAGTACAGCTTGTCCATCAGAGGTTCCACT ACCTTGCGCTTCTACCGGAACCTGAACCTAGAGGCCTGTTTATGGGAGTTCGTTAGCTACTATGACATG TCAGAACTCCTTGCTGACTGTGGTGGCACCATTGGAACAGATGGACAGGTCCTAAACCTAGTGCAGTCC TATGTGACCCTTCGAGTCCCTCTGTATGTTTCCTACGTGTTCCATTCCCCCGTGGGGGTAGGAGGCTGG CAGCATTTTGACTTGAAGTCAGAGCTTCGTCTAACTTTTGTGTACGACACCGCCATCTTGTGGAATGAT GGAATTGGCAGCCCCCCAGAGGCTGAACTTCAAGGTTCTCTCTATCCAACCAGCATGCGCATCGGTGAT GAGGGGCGCTTGGCCGTGCACTTCAAGACAGAGGCTCAGTTCCATGGCTTATTTGTGCTGTCACATCCC GCATCCTTTACAAGCTCAGTGATCATGTCAGCTGATCATCCAGGCCTGACATTTTCCCTCCGCCTCATA AGGAGTGAACCAACCTATAACCAGCCAGTACAGCAGTGGAGCTTTGTCTCTGACTTTGCCGTACGTGAC TACTCAGGGACCTATACTGTGAAGCTGGTGCCATGCACTGCCCCATCACATCAGGAATACCGCCTGCCA GTCACCTGCAACCCCAGAGAACCTGTCACCTTTGACCTTGACATCCGATTCCAACAGGTCAGTGATCCA GTGGCTGCTGAGTTTAGCTTGAACACCCAAATGTACCTGCTCTCTAAGAAGAGTCTCTGGTTGTCTGAT GGATCCATGGGATTCGGGCAAGAGAGTGATGTTGCTTTTGCAGAAGGTGATATAATTTATGGTCGTGTC ATGGTGGATCCTGTCCAGAATCTGGGTGACTCCTTTTACTGCAGCATTGAGAAGGTGTTTCTATGCACT GGAGCTGATGGCTATGTTCCCAAGTATAGTCCAATGAATGCAGAATATGGCTGCTTAGCCGACTCTCCT TCACTCTTATATAGATTTAAAATTGTGGACAAAGCTCAGCCAGAGACACAAGCGACCAGTTTTGGAAAT GTCCTATTTAATGCCAAACTAGCAGTGGATGACCCTGAAGCCATTCTCTTAGTGAATCAGCCTGGATCT GATGGATTTAAAGTCGACTCAACACCACTCTTTCAGGTCGCTCTAGGCCGAGAATGGTATATACATACG ATCTATACAGTGAGATCGAAAGACAATGCCAATCGAGGTATTGGCAAAAGAAGTGTGGAGTACCATTCT CTGGTGAGTCAAGGAAAGCCCCAATCCACCACCAAGAGCCGGAAGAAGAGAGAGATCAGGAGCACACCC TCACTGGCATGGGAGATTGGTGCTGAAAACAGTCGAGGAACAAACATCCAGCACATTGCCCTGGACCGC ACCAAGAGGCAGATCCCCCATGGGAGAGCACCTCCAGATGGCATCCTCCCCTGGGAGCTCAACAGCCCC AGCTCTGCAGTCAGCCTGGTCACTGTGGTGGGAGGCACCACGGTAGGGTTACTCACCATCTGCCTCACT GTCATTGCAGTGCTGATGTGCAGGGGCAAGGAAAGTTTCAGGGGGAAGGATGCCCCGAAAGGCTCCAGC AGCAGTGAGCCCATGGTGCCCCCACAGAGCCATCACAATGACAGCTCAGAAGTTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_207361
Insert Size	9510 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This clone has been fully sequenced and found three SNPs within the protein associated with this reference, NM_207361.3, and only one SNP changes amino acid.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_207361.3
RefSeq Size	9510 bp
RefSeq ORF	9510 bp
Locus ID	341640
UniProt ID	Q5SZK8
Protein Families	Transmembrane
MW	351.2 kDa
Gene Summary	This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014]

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