CRB1 (NM_201253) Human Untagged Clone
CAT＃: SC307996
CRB1 (untagged)-Human crumbs homolog 1 (Drosophila) (CRB1), transcript variant 1
CNY 14440.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRB1-A; CRB1-B; CRB1-C; LCA8; RP12 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_201253 edited
GCCACCATGGCACTTAAGAACATTAACTACCTTCTCATCTTCTACCTCAGTTTCTCACTG CTTATCTACATAAAAAATTCCTTTTGCAATAAAAACAACACCAGGTGCCTCTCAAATTCT TGCCAAAACAATTCTACATGCAAAGATTTTTCAAAAGACAATGATTGTTCTTGTTCAGAC ACAGCCAATAATTTGGACAAAGACTGTGACAACATGAAAGACCCTTGCTTCTCCAATCCC TGTCAAGGAAGTGCCACTTGTGTGAACACCCCAGGAGAAAGGAGCTTTCTGTGCAAATGT CCTCCTGGGTACAGTGGGACAATCTGTGAAACTACCATTGGTTCCTGTGGCAAGAACTCC TGCCAACATGGAGGTATTTGCCATCAGGACCCTATTTATCCTGTCTGCATCTGCCCTGCT GGATATGCTGGAAGATTCTGTGAGATAGATCACGATGAGTGTGCTTCCAGCCCTTGCCAA AATGGGGCCGTGTGCCAGGATGGAATTGATGGTTACTCCTGCTTCTGTGTCCCAGGATAT CAAGGCAGACACTGCGACTTGGAAGTGGATGAATGTGCTTCAGATCCCTGCAAGAACGAG GCTACATGCCTCAATGAAATAGGAAGATATACTTGTATCTGTCCCCACAATTATTCTGGT GTAAACTGTGAATTGGAAATTGACGAATGTTGGTCCCAGCCTTGTTTAAATGGTGCAACT TGTCAGGATGCTCTGGGGGCCTATTTCTGCGACTGTGCCCCTGGATTCCTGGGGGATCAC TGTGAACTCAACACTGATGAGTGTGCCAGTCAACCTTGTCTCCATGGAGGGCTGTGTGTG GATGGAGAAAACAGATATAGCTGTAACTGCACGGGTAGTGGATTCACAGGGACACACTGT GAGACCTTGATGCCTCTTTGTTGGTCAAAACCTTGTCACAATAATGCTACATGTGAGGAC AGTGTTGACAATTACACTTGTCACTGCTGGCCTGGATACACAGGTGCCCAGTGTGAGATC GACCTCAATGAATGCAATAGTAACCCCTGCCAGTCCAATGGGGAATGTGTGGAGCTGTCC TCAGAGAAACAATATGGACGCATCACTGGACTGCCTTCTTCTTTCAGCTACCATGAAGCC TCAGGTTATGTCTGTATCTGTCAGCCTGGATTCACAGGAATCCACTGCGAAGAAGACGTC AATGAATGTTCTTCAAACCCTTGCCAAAATGGTGGTACTTGTGAGAACTTGCCTGGGAAT TATACTTGCCATTGCCCATTTGATAACCTTTCTAGAACTTTTTATGGAGGAAGGGACTGT TCTGATATTCTCCTGGGCTGTACCCATCAGCAATGTCTAAATAATGGAACATGCATCCCT CACTTCCAAGATGGCCAGCATGGATTCAGCTGCCTGTGTCCATCTGGCTACACCGGGTCC CTGTGTGAAATCGCAACCACACTTTCATTTGAGGGCGATGGCTTCCTGTGGGTCAAAAGT GGCTCAGTGACAACCAAGGGCTCAGTTTGTAACATAGCCCTCAGGTTTCAGACTGTTCAG CCAATGGCTCTTCTACTTTTCCGAAGCAACAGGGATGTGTTTGTGAAGCTGGAGCTGCTA AGTGGCTACATTCACTTATCAATTCAGGTCAATAATCAGTCAAAGGTGCTTCTGTTCATT TCCCACAACACCAGCGATGGAGAGTGGCATTTCGTGGAGGTAATATTTGCAGAGGCTGTG ACCCTTACCTTAATCGACGACTCCTGTAAGGAGAAATGCATCGCGAAAGCTCCTACTCCA CTTGAAAGTGATCAATCAATATGTGCTTTTCAGAACTCCTTTTTGGGTGGTTTACCAGTG GGAATGACCAGCAATGGTGTTGCTCTGCTTAACTTCTATAATATGCCATCCACACCTTCG TTTGTAGGCTGTCTCCAAGACATTAAAATTGATTGGAATCACATTACCCTGGAGAACATC TCGTCTGGCTCATCATTAAATGTCAAGGCAGGCTGTGTGAGAAAGGATTGGTGTGAAAGC CAACCTTGTCAAAGCAGAGGACGCTGCATCAACTTGTGGCTGAGTTACCAGTGTGACTGC CACAGGCCCTATGAAGGCCCCAACTGTCTGAGAGAGTATGTGGCAGGCAGATTTGGCCAG GATGACTCCACTGGTTATGTCATCTTTACTCTTGATGAGAGCTATGGAGACACCATCAGC CTCTCCATGTTTGTCCGAACGCTTCAACCATCAGGCTTACTTCTAGCTTTGGAAAACAGC ACTTATCAATATATCCGTGTCTGGCTAGAGCGCGGCAGACTAGCAATGCTGACTCCAAAC TCTCCCAAATTAGTAGTAAAATTTGTTCTTAATGATGGAAATGTCCACTTGATATCTTTG AAAATCAAGCCATATAAAATTGAACTGTATCAGTCTTCACAAAACCTAGGATTTATTTCT GCTTCTACGTGGAAAATCGAAAAGGGAGATGTCATCTACATTGGTGGCCTACCTGACAAG CAAGAGACTGAACTTAATGGTGGATTCTTCAAAGGCTGTATCCAAGATGTAAGACTAAAC AACCAAAATCTGGAATTCTTTCCAAATCCAACAAACAATGCATCTCTCAATCCAGTTCTT GTCAATGTAACCCAAGGCTGTGCTGGAGACAACAGCTGCAAGTCCAACCCCTGTCACAAT GGAGGTGTTTGCCATTCCCGGTGGGATGACTTCTCCTGTTCCTGTCCTGCCCTCACAAGT GGGAAAGCCTGTGAGGAGGTTCAGTGGTGTGGATTCAGCCCGTGTCCTCACGGAGCCCAG TGCCAGCCGGTGCTTCAAGGATTTGAATGTATTGCAAATGCTGTTTTTAATGGACAAAGC GGTCAAATATTATTCAGAAGCAATGGGAATATTACCAGAGAACTCACCAATATCACATTT GGTTTCAGAACAAGGGATGCAAATGTAATAATATTGCATGCAGAAAAAGAGCCTGAATTT CTTAATATTAGCATTCAAGATTCCAGATTATTCTTTCAATTGCAAAGTGGCAACAGCTTT TATATGCTAAGTCTGACAAGTTTGCAGTCAGTGAATGATGGCACATGGCACGAAGTGACC CTTTCCATGACAGACCCACTGTCCCAGACCTCCAGGTGGCAAATGGAAGTGGACAACGAA ACACCTTTTGTGACCAGCACAATTGCTACTGGAAGCCTCAACTTTTTGAAGGATAATACA GATATTTATGTGGGAGACAGAGCTATTGACAATATAAAGGGCCTGCAAGGGTGTCTAAGT ACAATAGAAATCGGAGGCATTTATCTCTCTTACTTTGAAAATGTTCATGGTTTCATTAAT AAACCTCAGGAAGAGCAATTTCTCAAAATCTCTACCAATTCAGTGGTCACTGGCTGTTTG CAGTTAAATGTCTGCAACTCCAACCCCTGTTTGCATGGAGGAAACTGTGAAGACATCTAT AGCTCTTATCATTGCTCCTGTCCCTTGGGATGGTCAGGGAAACACTGTGAACTCAACATC GATGAATGCTTTTCAAACCCCTGTATCCATGGCAACTGCTCTGACAGAGTTGCAGCCTAC CACTGCACATGTGAGCCTGGATACACTGGTGTGAACTGTGAAGTGGATATAGACAACTGC CAGAGTCACCAGTGTGCAAATGGAGCCACCTGCATTAGTCATACTAATGGCTATTCTTGC CTCTGTTTTGGAAATTTTACAGGAAAATTTTGCAGACAGAGCAGATTACCCTCAACAGTC TGTGGGAATGAGAAGACAAATCTCACTTGCTACAATGGAGGCAACTGCACAGAGTTCCAG ACTGAATTAAAATGTATGTGCCGGCCAGGTTTTACTGGAGAATGGTGTGAAAAGGACATT GATGAGTGTGCCTCTGATCCGTGTGTCAATGGAGGTCTGTGCCAGGACTTACTCAACAAA TTCCAGTGCCTCTGTGATGTTGCCTTTGCTGGCGAGCGCTGCGAGGTGGACTTGGCAGAT GACTTGATCTCCGACATTTTCACCACTATTGGCTCAGTGACTGTCGCCTTGTTACTGATC CTCTTGCTGGCCATTGTTGCTTCTGTTGTCACCTCCAACAAAAGGGCAACTCAGGGAACC TACAGCCCCAGCCGTCAGGAGAAGGAGGGCTCCCGAGTGGAAATGTGGAACTTGATGCCA CCCCCTGCAATGGAGAGACTGATTTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_201253 |
| Insert Size | 4300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_201253.1, NP_957705.1 |
| RefSeq Size | 4950 bp |
| RefSeq ORF | 4221 bp |
| Locus ID | 23418 |
| UniProt ID | P82279 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.[provided by RefSeq, Apr 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212347 | CRB1 (Myc-DDK-tagged)-Human crumbs homolog 1 (Drosophila) (CRB1), transcript variant 1 |
CNY 14432.00 |
|
| RC212347L3 | Lenti-ORF clone of CRB1 (Myc-DDK-tagged)-Human crumbs homolog 1 (Drosophila) (CRB1), transcript variant 1 |
CNY 15960.00 |
|
| RC212347L4 | Lenti-ORF clone of CRB1 (mGFP-tagged)-Human crumbs homolog 1 (Drosophila) (CRB1), transcript variant 1 |
CNY 15960.00 |
|
| RG212347 | CRB1 (tGFP-tagged) - Human crumbs homolog 1 (Drosophila) (CRB1), transcript variant 1 |
CNY 15490.00 |
