SP8 (NM_182700) Human Untagged Clone
CAT＃: SC307485
SP8 (untagged)-Human Sp8 transcription factor (SP8), transcript variant 1
CNY 8080.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BTD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC307485 representing NM_182700.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAACTTCACTTCTAGGGGAAGAACCGAGGTTGGGATCGACTCCTCTGGCCATGCTTGCCGCTACC TGTAATAAGATAGGCAGCCCCAGCCCGTCTCCCTCCTCCCTCTCGGACAGCTCTTCTTCCTTCGGCAAA GGCTTCCACCCCTGGAAACGCTCCTCGTCCTCTTCTTCCGCCAGCTGCAACGTAGTGGGTTCCAGTCTC TCAAGCTTCGGCGTGTCCGGGGCCTCCAGGAACGGCGGCTCGTCCTCGGCGGCTGCGGCGGCCGCGGCA GCAGCCGCGGCTGCCGCGGCCCTGGTGTCCGACTCGTTCAGCTGCGGCGGCTCGCCTGGCTCCAGCGCC TTCTCCCTCACCTCCAGCAGCGCCGCAGCCGCCGCCGCCGCCGCCGCAGCCGCCGCCTCCAGCTCGCCC TTCGCCAACGACTACTCTGTTTTCCAGGCCCCCGGAGTTTCCGGGGGCAGCGGCGGCGGCGGCGGGGGC GGCGGCGGCGGCTCCTCCGCGCACTCGCAGGACGGCTCCCACCAGCCGGTGTTCATCTCCAAGGTGCAC ACCTCTGTGGACGGGCTGCAGGGCATCTACCCGCGGGTGGGCATGGCGCACCCGTACGAGTCGTGGTTT AAGCCCTCGCACCCGGGCCTGGGTGCTGCGGGCGAGGTGGGCTCGGCCGGCGCCTCCAGCTGGTGGGAC GTGGGGGCCGGCTGGATCGACGTGCAGAACCCGAACAGCGCGGCTGCGCTGCCCGGCTCGCTGCACCCT GCCGCCGGGGGGCTCCAAACCTCGCTGCACTCGCCGCTCGGAGGCTACAACTCGGATTACTCGGGCCTG AGTCACTCGGCCTTCAGCAGCGGCGCCTCCTCGCACCTGCTCAGCCCCGCCGGGCAGCACCTCATGGAC GGCTTCAAGCCAGTGCTACCCGGCTCCTACCCGGACTCGGCCCCGTCGCCGCTGGCCGGCGCGGGGGGC TCCATGTTGAGCGCTGGGCCTTCGGCGCCGCTGGGGGGCTCCCCGCGCTCCTCAGCTCGCCGCTACTCC GGCCGCGCCACCTGCGACTGCCCCAACTGCCAGGAGGCAGAGCGGCTGGGCCCTGCCGGGGCGAGCTTG CGGCGCAAGGGCCTGCACAGCTGCCACATCCCGGGCTGCGGCAAGGTGTACGGCAAGACTTCGCACCTC AAGGCGCACCTGCGCTGGCACACGGGCGAGCGGCCCTTCGTGTGCAACTGGCTTTTCTGCGGCAAGCGC TTCACGCGCTCCGACGAGCTGCAGCGGCACCTGCGGACCCACACCGGCGAGAAGCGCTTCGCCTGTCCA GTTTGCAACAAGCGCTTCATGCGCAGCGACCACCTCAGCAAGCACGTGAAGACGCACAGTGGCGGCGGC GGCGGCGGCGGCTCGGCGGGCTCGGGCAGCGGCGGCAAGAAGGGCAGCGACACCGACAGCGAGCACAGC GCCGCGGGCAGCCCGCCCTGCCACTCCCCAGAGCTGCTGCAGCCCCCCGAGCCCGGGCACCGCAACGGC CTAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_182700 |
| Insert Size | 1527 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182700.5 |
| RefSeq Size | 3643 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 221833 |
| UniProt ID | Q8IXZ3 |
| MW | 50.5 kDa |
| Gene Summary | The protein encoded by this gene is an SP family transcription factor that in mouse has been shown to be essential for proper limb development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212880 | SP8 (Myc-DDK-tagged)-Human Sp8 transcription factor (SP8), transcript variant 1 |
CNY 3784.00 |
|
| RC212880L3 | Lenti ORF clone of Human Sp8 transcription factor (SP8), transcript variant 1, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC212880L4 | Lenti ORF clone of Human Sp8 transcription factor (SP8), transcript variant 1, mGFP tagged |
CNY 6460.00 |
|
| RG212880 | SP8 (tGFP-tagged) - Human Sp8 transcription factor (SP8), transcript variant 1 |
CNY 5040.00 |
