USH1G (NM_173477) Human Untagged Clone
CAT＃: SC306825
USH1G (untagged)-Human Usher syndrome 1G (autosomal recessive) (USH1G)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANKS4A; SANS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306825 representing NM_173477.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACGACCAGTACCACCGGGCAGCCCGGGATGGCTACCTGGAGCTCCTCAAGGAGGCCACCCGAAAG GAGCTGAATGCCCCCGACGAGGATGGCATGACCCCCACTCTCTGGGCTGCCTACCATGGCAACCTCGAG TCGCTGCGTCTCATTGTGAGCCGCGGGGGTGACCCGGACAAGTGTGACATCTGGGGCAACACACCCCTG CATCTGGCAGCTTCCAATGGCCACTTGCACTGCCTGTCCTTCCTGGTGTCCTTCGGAGCCAACATCTGG TGCCTAGACAACGACTACCACACGCCGCTGGACATGGCTGCCATGAAGGGCCACATGGAATGCGTGCGC TACCTGGACTCCATCGCGGCCAAGCAGAGCAGCCTCAACCCCAAGCTGGTGGGTAAGCTGAAGGACAAG GCCTTCCGCGAGGCGGAGCGGCGCATCCGCGAGTGCGCCAAGCTGCAGCGGAGGCACCACGAACGCATG GAGCGGCGATACCGGCGCGAGCTGGCCGAGCGTTCCGACACCCTCAGCTTCTCCAGCCTCACGTCCAGC ACCCTGAGCCGCCGGCTGCAGCATCTGGCGCTGGGCAGCCACCTGCCGTACTCTCAGGCCACGCTGCAC GGCACGGCCAGGGGCAAGACCAAGATGCAGAAGAAGCTGGAGCGGCGCAAGCAGGGCGGCGAAGGCACC TTCAAGGTCTCCGAGGATGGGCGCAAGAGCGCCCGCTCGCTCTCGGGCCTGCAGCTGGGCAGCGACGTG ATGTTCGTGCGCCAGGGCACCTACGCCAATCCCAAGGAGTGGGGCCGAGCCCCGCTCCGGGACATGTTC CTCTCGGACGAGGACAGCGTCTCCCGTGCCACGCTGGCGGCCGAGCCTGCCCACTCGGAGGTCAGCACC GACTCAGGCCACGACTCCCTGTTTACCCGCCCCGGCCTGGGCACCATGGTGTTCCGCAGAAATTACTTG AGCAGTGGGCTGCACGGACTGGGCCGCGAGGATGGGGGTCTGGATGGGGTGGGAGCGCCGCGGGGTCGG CTGCAGAGCTCCCCCAGCCTGGACGATGACAGCCTGGGCAGTGCCAACAGCCTGCAGGACCGCAGCTGT GGGGAGGAGCTGCCCTGGGATGAGCTCGATTTAGGCTTGGACGAGGACCTGGAGCCCGAGACTAGCCCG CTGGAGACCTTCCTGGCCTCTCTGCACATGGAGGACTTTGCCGCCCTCCTGCGGCAGGAGAAGATCGAC CTCGAGGCTTTGATGCTGTGCTCTGACCTCGACCTCCGCAGCATCAGCGTCCCACTGGGGCCCCGAAAG AAGATCTTGGGGGCCGTGAGGAGGCGGCGGCAGGCGATGGAGCGCCCGCCGGCCCTGGAGGACACAGAG CTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173477 |
| Insert Size | 1386 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173477.4 |
| RefSeq Size | 3565 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 124590 |
| UniProt ID | Q495M9 |
| MW | 51.5 kDa |
| Gene Summary | This gene encodes a protein that contains three ankyrin domains, a class I PDZ-binding motif and a sterile alpha motif. The encoded protein interacts with harmonin, which is associated with Usher syndrome type 1C. This protein plays a role in the development and maintenance of the auditory and visual systems and functions in the cohesion of hair bundles formed by inner ear sensory cells. Mutations in this gene are associated with Usher syndrome type 1G (USH1G). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218255 | USH1G (Myc-DDK-tagged)-Human Usher syndrome 1G (autosomal recessive) (USH1G) |
CNY 3656.00 |
|
| RC218255L1 | Lenti ORF clone of Human Usher syndrome 1G (autosomal recessive) (USH1G), Myc-DDK-tagged |
CNY 6056.00 |
|
| RC218255L2 | Lenti ORF clone of Human Usher syndrome 1G (autosomal recessive) (USH1G), mGFP tagged |
CNY 5890.00 |
|
| RC218255L3 | Lenti ORF clone of Human Usher syndrome 1G (autosomal recessive) (USH1G), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC218255L4 | Lenti ORF clone of Human Usher syndrome 1G (autosomal recessive) (USH1G), mGFP tagged |
CNY 5890.00 |
|
| RG218255 | USH1G (tGFP-tagged) - Human Usher syndrome 1G (autosomal recessive) (USH1G) |
CNY 4370.00 |
