KNDC1 (NM_152643) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bB439H18.3; C10orf23; RASGEF2; v-KIND; Very-KIND
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC306472 representing NM_152643. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGGCCATGGACCCGGCCGCGGCGGATCTTTACGAGGAGGACGGCAAAGACCTGGACTTCTACGAC TTCGAGCCGCTGCCCACCCTCCCCGAGGACGAGGAGAACGTGTCTCTGGCTGACATCCTCTCCCTGCGG GACCGCGGCCTCAGCGAGCAGGAAGCCTGGGCCGTGTGCCTGGAGTGCAGCCTGTCCATGCGGAGCGTG GCCCACGCCGCCATCTTCCAGAGCCTGTGCATCACGCCCGACACCCTGGCCTTCAACACCAGCGGGAAC GTGTGTTTCATGGAGCAGCTCAGCGACGACCCTGAGGGTGCCTTCGTTCCCCCCGAGTTCGACGTGACC GGGAACACCTTTGAGGCGCACATCTACTCTCTGGGGGCCACGCTGAAGGCCGCCCTCGAGTACGTGGCA GAGCCCACACTGGAACCCAGGCTGAGCCAAGACCTCGAGGCGCTGCTGAGCCGGATGCAGGCGGAGGAC CCCGGGGACCGGCCGGACCTTGAGAGCATCATCGCGCTGTGTGAAGAGAAGCTGCAGCTCACATCCTCC TGTCGCGTGTGCCGGAGCCTCTCTGCTGTGGGGAGGAGGGTCCTCTCCATCGAGTCCTTCGGAGCGCTG CAGGATGTCAGCGAGAGCAGCTGGCGGGAGAGACCTGCCCCAGGAAACGCTGGGCCCAGGAGGCCGCCC GGGGACCCCAGCACTGACCCGGAGGTTCTGCCGACCCCCGAAGGCCCGGAGTCTGAGACGAGCCGGGGC CCCAGAGCCTCCCCAACCAAGGCTCTGCTGTCCACCCCGGTGAGAAATGGCGAGAGCCACAGCCGGGAG GGGCTGGCCGGCCTCGTCCTGGATGCCGAGCGCACCCTCGGGGAGCTGGACAGAGACGCCCTCAGGAGA AGCCGCCTGCGGAAGGTGCAGACGTTCCCTAGGCTGCTGTCCGACAGCCCCGAGGCCACCCTCTGCCTG CCGCTGACCCGCGGGAAAAGCCAGCTGCCCATATCGGAATTATTCTCTCCGGACCCCAGGAAGGCCTTT CTGGACAGGAAAAATGGCCTTTCTAGCTTCCAGGCTCAGCCCAAATGCAGGCTGTGGCCGGAGCAGGAG CCGGAACACCAGCTGGGACGGGTTCCCTGTGCAGGCCGCAGCACGGACAGGGGCCCTGGGGTGCCCGGC AGTCCAGGACAGCCCGAGACTTCACACCCCAGCCAGGGGCCAGCAGAGGCCCCTGCAGACCCTCGAGAT GCTAGCGGTGAAGCCCAGACTCCCAGGGACGATGAGAGAATTCCAGAAGGAGCTAGGCAGCTGGAAAGT GCAGCCGCGGAGCAGTGGGTGTCCCTGCAGGACCTCCTGTCCCAGCTGGGCCGGCCCTTCCGGGAGTAC GAGCTGTGGGCCCTGTGCCTGGCCTGCCTCCGCGCACTGCAGACACGCCCTGAGCACCCAGCCTACCTG TGTCTGGACTCCGTGCTGGTTGCTGAGGACGGGGCTGTGCTCTTCCAGCCACCCCCTGCCAACGGTTCC TATGACTCGTTCTTTCTGGCTCCCGAGCTGGCAGAGGAGAGGCTGGTAACTGAAAAGGCCTCTGTGTAC TGTGTGGCCGCCGTTCTGTGGACCGCAGCCAAGTTCAGCGTCCCCCGCAACCACAAGCTGGCCCTGCCA CGCAGGCTCAAGACCCTCCTCCTGGACATGGCCAGGCGCAGTGCCCCGGAGCGGCCGTCCGCGGCTGAG GCCATCAAGGTGTGCGGCAGCTACCTCCTCCAGCGAGGCATGGACAGCCGGAAAATCCTTGCCCACCTC AGAGCTTCCATCTGCCAGGTGTACCAGGAGGAAGAGACCATCAGCCTCCAAAACGCCTTCTCAGTGGTT GAACTGAAGCCCAGTGTGGCACCAGCCCCAGAGCCCAGCCCAGGCTTCCTGCCGGTGAACAGCGACACC GGGCTTGTGGCTGTGCCAGGGCCCGTGCCCGGCCAGCACCCCTGCGGTGAAGAAGCCACCCAGCTGCCT GCAGCGTTCACCTCCGAGGCCACGCACTTCAAGCCCATTGTCCTCGCGCAGAACGCAAGTGTGGCCAGG GACCAGCCTGCCTTGGCCCAGGAGGAGTCCGAGGAGAGGGGCGGCCAGAGGGAGGGAGAAGGTGAGGAG AAGCTCTCCCTGGAGGCCCACGCTGGGTCCCCCAGCCTGAAGACGCCTGACGGGCCGGTGCCTGGTCCG GGGCCACAGGGAGCAGCCCCAGAGCCTCTTGGGGCGTCAGTGCAGCGTGACTCAGCCCAGGGCCGCCCC TGCCCTCCACCCCAGGCCCCAGCAAACCAGCCAGAGGGGGCCTCATCGGCAGCTCCCGGCTCTCCAGTC CCCGCCCCGCCCACGAAGGCATCTGCGCTGCCCGTAGAGCAAGGGCCGGCTGAGCCGATCCCACCTGGA GTTGCTTCCGGGGGCCTCAGGCCCGACGCCCTGGGGCCCACCACGGCCCACCACGGCCCACGCCACCCG CCCAAGCCCCCACGAAGCAAGGCCACCGAGCGCCCGGGCCAGGAGCCAGAGGGCCCCGGGGCCACCCCT GCCGGGGAACGTGATGACCAGAGTCCAGACAGTGTCCCAGAGAGGCCGCGGCCCGCAGACCGGAGGCTC TGTCTGCCCTGCGTGGATGCCTCGCCACTCCCAGGGAGGACGGCCTGCCCGTCGCTGCAGGAGGCCACG CGCCTCATCCAGGAGGAATTTGCCTTCGATGGCTACCTGGACAATGGGCTGGAGGCTCTGATCATGGGG GAGTACATCTTCGCCTTGAAAGATCTCACCTTTGCCACTTTCTGTGGCGCCATTTCCGAGAAGTTCTGT GACCTGTACTGGGATGAGAAGTTGCTGCAGAACCTCTTTAAGGTGGTCAACGGGCAGGCGTCACCCTCC CCAAGCACGGCCGAGGAGGCTGGGTCACAGCTCGAGGGCAGCCAAAGCCCCCGCTCCCCGTCCAGCAAG AGGCCGTCGCTGCACCGCCTGGGAAAAGAGAAGCCAGCCATGGCCAGGACCAGCAGCAGGGCCCCCTGC TCACCCACCTCGGTGTCGGATGTGGACTCGGACGCACTGTCACGGGGAAACTTCGAGGTGGGGTTTCGG CCTCAGAGGTCCGTAAAAGCCGAGAGAGCGCAGCAGCCTGAGGCTGGCGAGGACAGACGGCCAGCTGGC GGGGCCTCAGACGTGGAGGCAGTGACCCGACTGGCCAGGTCCAAAGGGGTCGGCCCAGCCTTGTCCCCC GGCCCAGCCGGATTCCAGAGCTGCAGCCCCGGCTGGTGCAGCGCCTTCTACGAGGCCGACTGCTTCGGG GCCGACGTCCACAACTACGTGAAGGACCTGGGGCGGCAGCAGGCGGACGGGGCCCTGCCCGACGCCCAG AGCCCGGAGCTGGAACAGCAGCTCATGATGGAGAAAAGAAACTACCGCAAGACCCTGAAGTTCTACCAG AAACTCTTACAGAAGGAAAAGAGGAACAAAGGTTCCGACGTCAAGACCATGCTGTCCAAGCTGAAAGGG CAGCTAGAAGAAATGAAATCCAGGGTGCAATTCCTCAGCTTGGTCAAGAAGTATCTGCAGGTCATGTAC GCGGAACGCTGGGGCCTGGAGCCCTGCACCCTCCCAGTGATCGTGAACATCGCGGCCGCACCCTGCGAC ACGCTGGACTTCAGCCCCCTGGACGAGTCCTCCTCGCTCATCTTCTACAACGTCAACAAGCACCCGGGC GGCCGGCAGAAGGCCCGCATCCTGCAGGCCGGCACGCCGCTGGGGCTCATGGCCTACCTGTACTCCAGT GATGCCTTCCTGGAGGGTTATGTGCAGCAATTCCTCTACACCTTCCGCTACTTCTGCACACCCCACGAC TTCCTGCACTTCCTCCTCGACCGCATCAACAGCACGCTGACCAGGGCCCACCAGGACCCCACCTCGACC TTCACCAAGATCTACAGGCGGAGCCTCTGCGTCCTGCAGGCCTGGGTGGAGGACTGCTACGCTGTGGAC TTCCCTCGGAACAGCGGGCTGCTGGGGAAGCTAGAGGACTTCATCTCCTCCAAGATCCTACCCCTGGAC GGCTCTGCCAAGCACCTGCTGGGCCTCCTGGAGGTGGGCATGGACCGGCGGGCCGAGGGCAACCCTCGC GGCACAGACCTGGAGAACCCCAGGGAGGCCGAGGAGGATGCCAGACCCTTCAACGCCCTCTGTAAGAGG CTCTCAGAGGACGGCATCTCCAGGAAGAGCTTCCCCTGGAGGCTGCCCCGAGGCAACGGGCTGGTGCTG CCGCCACACAAGGAGCGCCCCTACACCATTGCTGCCGCCCTGCCCAAGCCCTGCTTCCTCGAGGACTTC TACGGCCCCTGCGCCAAGACCAGTGAGAAGGGGCCCTACTTCCTGACGGAGTACAGCACTCACCAGCTC TTCAGCCAGCTCACGCTGCTACAGCAGGAGTTGTTTCAAAAGTGCCACCCGGTCCACTTCCTGAACTCA CGGGCCCTGGGCGTCATGGACAAGAGCACTGCCATCCCCAAAGCCAGCTCTTCTGAGTCTCTTTCGGCC AAAACCTGCAGCTTATTTCTGCCCAATTACGTTCAGGACAAGTATCTGTTACAGCTTCTAAGAAACGCA GATGACGTCAGCACCTGGGTGGCTGCAGAGATTGTGACCAGCCACACCTCCAAGCTGCAGGTGAACTTG CTGTCCAAATTTTTGCTGATTGCAAAATCTTGCTATGAGCAGAGAAACTTCGCGACAGCCATGCAGATC CTGAGCGGGCTGGAGCACCTGGCCGTGAGGCAGTCCCCTGCCTGGAGAATTCTGCCTGCAAAGATAGCA GAGGTCATGGAGGAGCTGAAAGCCGTGGAGGTCTTCCTGAAGAGCGACAGCCTGTGTCTGATGGAAGGG CGGCGCTTCCGGGCGCAGCCCACCCTGCCCTCGGCCCACCTCCTGGCCATGCACATCCAGCAGCTGGAG ACAGGCGGCTTCACCATGACCAACGGGGCCCACAGGTGGAGCAAGCTCAGGAACATCGCAAAGGTGGTG AGCCAGGTGCACGCGTTCCAGGAGAACCCTTACACCTTCAGCCCCGACCCCAAGCTCCAGTCGTACCTC AAGCAGAGGATTGCCCGCTTCAGCGGTGCCGACATTTCCACACTCGCCGCAGATAGCAGGGCCAACTTC CACCAGGTCTCCAGCGAGAAGCACTCACGGAAGATTCAGGACAAGCTACGGAGGATGAAGGCTACATTC CAGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_152643
Insert Size	5250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_152643.6
RefSeq Size	6773 bp
RefSeq ORF	5250 bp
Locus ID	85442
UniProt ID	Q76NI1
MW	191.4 kDa
Gene Summary	The protein encoded by this gene is a Ras guanine nucleotide exchange factor that appears to negatively regulate dendritic growth in the brain. Knockdown of this gene in senescent umbilical vein endothelial cells partially reversed the senescence, showing that this gene could potentially be targeted by anti-aging therapies. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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