WDR65 (CFAP57) (NM_152498) Human Untagged Clone
CAT＃: SC306421
WDR65 (untagged)-Human WD repeat domain 65 (WDR65), transcript variant 1
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | VWS2; WDR65 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306421 representing NM_152498.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCAGCCGTGGTAGCTCAGACGCTGCATGTTTTTGGTCTTCGATCCCACGTGGCCAACAATATCTTC TACTTCGATGAACAGATCATTATATTTCCTTCAGGAAATCACTGTGTGAAGTACAATGTGGATCAGAAA TGGCAAAAATTCATTCCAGGCTCAGAGAAGAGTCAGGGCATGTTGGCCTTGTCCATCAGTCCCAATCGG CGGTACCTCGCTATCTCTGAGACTGTGCAAGAAAAACCTGCCATCACCATTTATGAATTGTCATCCATC CCTTGCCGGAAGCGCAAAGTTCTTAATAATTTTGACTTCCAAGTTCAGAAATTTATTAGCATGGCTTTT TCTCCAGACTCCAAATACCTATTGGCTCAGACGTCACCTCCAGAGTCAAATCTTGTCTACTGGCTGTGG GAAAAACAGAAAGTAATGGCCATTGTTAGAATCGACACTCAGAACAACCCTGTCTACCAGGTGAGCTTC AGTCCACAGGATAACACTCAGGTGTGTGTCACTGGAAATGGGATGTTTAAGCTTCTCCGTTTTGCTGAG GGAACCCTGAAGCAAACCAGCTTTCAGAGGGGAGAACCCCAAAACTATCTAGCTCACACCTGGGTGGCT GATGACAAGATTGTCGTTGGCACTGACACAGGCAAACTCTTCCTCTTTGAATCTGGAGATCAGCGTTGG GAGACCAGCATAATGGTCAAGGAACCTACCAATGGCTCAAAGAGCCTGGATGTCATTCAGGAATCAGAG AGCCTGATTGAATTTCCACCAGTCAGTTCTCCACTCCCTTCCTATGAACAGATGGTGGCGGCCAGTAGC CATAGCCAGATGTCCATGCCCCAGGTGTTTGCCATTGCAGCCTATTCAAAGGGATTTGCCTGTTCTGCT GGGCCAGGGAGAGTTCTGCTGTTTGAGAAGATGGAAGAAAAGGATTTTTACCGTGAGAGCAGAGAAATC AGGATTCCTGTGGACCCGCAGAGCAATGATCCAAGTCAGTCTGACAAACAGGACGTTCTCTGCCTGTGC TTCAGCCCCTCAGAGGAAACTCTGGTTGCCAGCACCAGTAAGAACCAACTCTACAGCATCACCATGTCC CTGACAGAGATCAGCAAGGGGGAGCCTGCTCACTTTGAGTATTTGATGTATCCATTGCACTCAGCACCC ATCACCGGTCTAGCTACCTGCATCCGCAAACCCCTTATAGCCACCTGTTCTCTGGATCGATCCATCCGC CTTTGGAATTATGAAACAAACACCCTGGAACTATTTAAGGAATACCAAGAAGAGGCATATTCCATCAGC CTTCATCCATCTGGACACTTCATTGTAGTAGGGTTTGCTGACAAACTACGCCTCATGAATCTACTCATT GATGATATACGTTCTTTCAAAGAATACTCTGTTAGAGGATGCGGAGAGTGTTCCTTTAGCAATGGAGGT CACCTGTTTGCTGCAGTCAATGGAAATGTGATTCACGTTTACACCACCACGAGCCTAGAGAACATCTCA AGCCTGAAAGGACACACAGGGAAGATTCGCTCAATTGTGTGGAATGCAGATGATAGCAAACTGATTTCT GGTGGCACAGATGGTGCTGTGTATGAATGGAATCTGTCCACAGGAAAGAGAGAGACAGAATGCGTGCTC AAGTCTTGCAGCTACAACTGTGTTACTGTCTCCCCCGATGCCAAAATTATCTTTGCTGTTGGATCAGAC CACACCCTCAAGGAGATTGCAGATTCCTTGATCCTTCGAGAGATATCGGCGTTTGATGTCACCTACACC GCCATTGTCATCTCGCATTCTGGACGCATGATGTTTGTGGGCACCTCGGTGGGAACCATTCGTGCCATG AAGTACCCTCTGCCTCTGCAGAAGGAATTCAATGAGTACCAGGCCCATGCCGGTCCTATCACCAAGGTG AGCAGGGCCCTCTCCCCAGGAACCCAGTCCCACACCTGCCTGCTACGTGCCTTGTTCATCCCTTCAACC TCCCAATGTCTTTTCTCTCTCCTTCTTCTCTCTTATTTATTCATCCATCATTCATTGAATCACCATCTA TTGACTATGAATATACTCTTTGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_152498 |
| Insert Size | 2097 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152498.3 |
| RefSeq Size | 3037 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 149465 |
| UniProt ID | Q96MR6 |
| MW | 78.2 kDa |
| Gene Summary | This protein encoded by this gene belongs to the WD repeat-containing family of proteins, which function in the formation of protein-protein complexes in a variety of biological pathways. This family member is thought to function in craniofacial development, possibly in the fusion of lip and palate. A missense mutation in this gene is associated with Van der Woude syndrome 2. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) differs in the 3' UTR and lacks several exons in the 3' coding region, but includes an alternate 3' exon, compared to variant 5. The resulting isoform (b, also known as WDR65b) has a shorter and distinct C-terminus, compared to isoform a. Both variants 1 and 3 encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214887 | WDR65 (Myc-DDK-tagged)-Human WD repeat domain 65 (WDR65), transcript variant 1 |
CNY 5112.00 |
|
| RC214887L3 | Lenti ORF clone of Human WD repeat domain 65 (WDR65), transcript variant 1, Myc-DDK-tagged |
CNY 7700.00 |
|
| RC214887L4 | Lenti ORF clone of Human WD repeat domain 65 (WDR65), transcript variant 1, mGFP tagged |
CNY 7700.00 |
|
| RG214887 | WDR65 (tGFP-tagged) - Human WD repeat domain 65 (WDR65), transcript variant 1 |
CNY 6370.00 |
