DGKB (NM_145695) Human Untagged Clone
CAT＃: SC306260
DGKB (untagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2
CNY 7220.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAGK2; DGK; DGK-BETA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306260 representing NM_145695.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACAAACCAGGAAAAATGGGCCCACCTCAGCCCTTCGGAATTTTCCCAACTTCAGAAATATGCTGAG TATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAAT CCTGAAGGGAAACAAGACATTCTTAACCAAACAATAGATTTTGAAGGTTTCAAACTATTCATGAAGACA TTCCTGGAAGCCGAGCTTCCTGATGATTTCACTGCACACCTTTTCATGTCATTTAGCAACAAGTTTCCT CATTCTAGTCCAATGGTAAAAAGTAAGCCTGCTCTCCTATCAGGCGGTCTGAGAATGAATAAAGGTGCC ATCACCCCTCCCCGGACTACTTCTCCTGCAAATACGTGTTCCCCAGAAGTAATCCATCTGAAGGACATT GTCTGTTACCTGTCTCTGCTTGAAAGAGGAAGACCTGAGGATAAGCTTGAGTTTATGTTTCGCCTTTAT GACACGGATGGGAATGGCTTCCTGGACAGCTCGGAGCTAGAAAATATCATCAGTCAGATGATGCATGTT GCAGAATACCTTGAGTGGGATGTCACTGAACTTAATCCAATCCTCCATGAAATGATGGAAGAAATTGAC TATGATCATGATGGAACCGTGTCTCTGGAGGAATGGATTCAAGGAGGAATGACAACGATTCCACTTCTT GTGCTCCTGGGCTTAGAAAATAACGTGAAGGATGATGGACAGCACGTGTGGCGACTGAAGCACTTTAAC AAACCTGCCTATTGCAACCTTTGCCTGAACATGCTGATTGGCGTGGGGAAGCAGGGCCTCTGCTGTTCC TTCTGCAAGTACACAGTCCATGAGCGCTGTGTGGCTCGAGCACCTCCCTCTTGCATCAAGACCTATGTG AAGTCCAAAAGGAACACTGATGTCATGCACCATTACTGGGTTGAAGGTAACTGCCCAACCAAGTGTGAT AAGTGCCACAAAACTGTTAAATGTTACCAGGGCCTGACAGGACTGCATTGTGTTTGGTGTCAGATCACA CTGCATAATAAATGTGCTTCTCATCTAAAACCTGAATGTGACTGTGGACCTTTGAAGGACCATATTTTA CCACCCACAACAATCTGTCCAGTGGTACTGCAGACTCTGCCCACTTCAGGAGTTTCAGTTCCTGAGGAA AGACAATCAACAGTGAAAAAGGAAAAGAGTGGTTCCCAGCAGCCAAACAAAGTGATTGACAAGAATAAA ATGCAAAGAGCCAACTCTGTTACTGTAGATGGACAAGGCCTGCAGGTCACTCCTGTGCCTGGTACTCAC CCACTTTTAGTTTTTGTGAACCCCAAAAGTGGTGGAAAACAAGGAGAACGAATTTACAGAAAATTCCAG TATCTATTAAATCCTCGTCAGGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTAAACTTTTTC CGTGATGTTCCTGACTTCAGAGTGTTAGCCTGTGGTGGAGATGGAACCGTGGGCTGGGTTTTGGATTGC ATAGAAAAGGCCAATGTAGGCAAGCATCCTCCAGTTGCGATTCTGCCTCTTGGGACTGGCAATGATCTA GCAAGATGCCTGCGATGGGGAGGAGGTTACGAAGGTGAGAATCTGATGAAAATTCTAAAAGACATTGAA AACAGCACAGAAATCATGTTGGACAGGTGGAAGTTTGAAGTCATACCTAATGACAAAGATGAGAAAGGA GACCCAGTGCCTTACAGTATCATCAATAATTACTTTTCCATTGGCGTGGATGCCTCCATTGCACACAGA TTCCACATCATGAGAGAAAAACACCCAGAGAAATTCAACAGTAGAATGAAGAACAAATTTTGGTATTTT GAGTTTGGCACATCTGAAACTTTCTCAGCCACCTGCAAGAAGCTACATGAATCTGTAGAAATAGAATGT GATGGAGTACAGATAGATTTAATAAACATCTCTCTGGAAGGAATTGCTATTTTGAATATACCAAGCATG CATGGAGGATCCAATCTTTGGGGAGAGTCTAAGAAAAGACGAAGCCATCGACGAATAGAGAAAAAAGGG TCTGACAAAAGGACCACCGTCACAGATGCCAAAGAGTTGAAGTTTGCAAGTCAAGATCTCAGTGACCAG CTGCTGGAGGTGGTCGGCTTGGAAGGAGCCATGGAGATGGGGCAAATATACACAGGCCTGAAAAGTGCT GGCCGGCGGCTGGCTCAGTGCTCCTGCGTGGTCATCAGGACGAGCAAGTCTCTGCCAATGCAAATTGAT GGGGAGCCATGGATGCAGACCCCATGCACAGTGAGTACAGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145695 |
| Insert Size | 2322 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145695.2 |
| RefSeq Size | 3360 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 1607 |
| UniProt ID | Q9Y6T7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 87.1 kDa |
| Gene Summary | Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (2) has a distinct 3' UTR and 3' coding region compared to variant (1). The encoded protein (2) has a shorter, alternate C-terminus compared to isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211233 | DGKB (Myc-DDK-tagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2 |
CNY 5664.00 |
|
| RC211233L3 | Lenti ORF clone of Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC211233L4 | Lenti ORF clone of Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2, mGFP tagged |
CNY 8064.00 |
|
| RG211233 | DGKB (tGFP-tagged) - Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2 |
CNY 7264.00 |
