RNF145 (NM_144726) Human Untagged Clone
CAT＃: SC306192
RNF145 (untagged)-Human ring finger protein 145 (RNF145), transcript variant 2
CNY 13400.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_144726, the custom clone sequence may differ by one or more nucleotides
ATGATGAGAAACCACAGAATTGCTAGTAGTTTATGTGGAGATCAGGTCTTCTCCAAGAAA AAAAAAAAGAAAAAAAAAAACAACATGGCTGCAAAGGAGAAACTGGAGGCAGTGTTAAAT GTGGCCCTGAGGGTGCCAAGCATCATGCTGTTGGATGTCCTGTACAGATGGGATGTCAGC TCCTTTTTCCAGCAGATCCAAAGAAGTAGCCTTAGTAATAACCCTCTTTTCCAGTATAAG TATTTGGCTCTTAATATGCATTATGTAGGTTATATCTTAAGTGTGGTGCTGCTAACATTG CCCAGGCAGCATCTGGTTCAGCTTTATCTATATTTTTTGACTGCTCTGCTCCTCTATGCT GGACATCAAATTTCCAGGGACTATGTTCGGAGTGAACTGGAGTTTGCCTATGAGGGACCA ATGTATTTAGAACCTCTCTCTATGAATCGGTTTACCACAGCCTTAATAGGTCAGTTGGTG GTGTGTACTTTATGCTCCTGTGTCATGAAAACAAAGCAGATTTGGCTGTTTTCAGCTCAC ATGCTTCCTCTGCTAGCACGACTCTGCCTTGTTCCTTTGGAGACAATTGTTATCATCAAT AAATTTGCTATGATTTTTACTGGATTGGAAGTTCTCTATTTTCTTGGGTCTAATCTTTTG GTACCTTATAACCTTGCTAAATCTGCATACAGAGAATTGGTTCAGGTAGTGGAGGTATAT GGCCTTCTCGCCTTGGGAATGTCCCTGTGGAATCAACTGGTAGTCCCTGTTCTTTTCATG GTTTTCTGGCTCGTCTTATTTGCTCTTCAGATTTACTCCTATTTCAGTACTCGAGATCAG CCTGCATCACGTGAGAGGCTTCTTTTCCTTTTTCTGACAAGTATTGCGGAATGCTGCAGC ACTCCTTACTCTCTTTTGGGTTTGGTCTTCACGGTTTCTTTTGTTGCCTTGGGTGTTCTC ACACTCTGCAAGTTTTACTTGCAGGGTTATCGAGCTTTCATGAATGATCCTGCCATGAAT CGGGGCATGACAGAAGGAGTAACGCTGTTAATCCTGGCAGTGCAGACTGGGCTGATAGAA CTGCAGGTTGTTCATCGGGCATTCTTGCTCAGTATTATCCTTTTCATTGTCGTAGCTTCT ATCCTACAGTCTATGTTAGAAATTGCAGATCCTATTGTTTTGGCACTGGGAGCATCTAGA GACAAGAGCTTGTGGAAACACTTCCGTGCTGTAAGCCTTTGTTTATTTTTATTGGTATTC CCTGCTTATATGGCTTATATGATTTGCCAGTTTTTCCACATGGATTTTTGGCTTCTTATC ATTATTTCCAGCAGCATTCTTACCTCTCTTCAGGTTCTGGGAACACTTTTTATTTATGTC TTATTTATGGTTGAGGAATTCAGAAAAGAGCCAGTGGAAAACATGGATGATGTCATCTAC TATGTGAATGGCACTTACCGCCTGCTGGAGTTTCTTGTGGCCCTCTGTGTGGTGGCCTAT GGCGTCTCAGAGACCATCTTTGGAGAATGGACAGTGATGGGCTCAATGATCATCTTCATT CATTCCTACTATAACGTGTGGCTTCGGGCCCAGCTGGGGTGGAAGAGCTTTCTTCTCCGC AGGGATGCTGTGAATAAGATTAAATCGTTACCCATTGCTACGAAAGAGCAGCTTGAGAAA CACAATGATATTTGTGCCATCTGTTATCAGGACATGAAATCTGCTGTGATCACGCCTTGC AGTCATTTTTTCCATGCAGGCTGTCTTAAGAAATGGCTGTATGTCCAGGAGACCTGCCCT CTGTGCCACTGCCATCTGAAAAACTCCTCCCAGCTTCCAGGATTAGGAACTGAGCCAGTT CTACAGCCTCATGCTGGAGCTGAGCAAAACGTCATGTTTCAGGAAGGTACTGAACCCCCA GGCCAGGAGCATACTCCAGGGACCAGGATACAGGAAGGTTCCAGGGACAATAATGAGTAC ATTGCCAGACGACCAGATAACCAGGAAGGGGCTTTTGACCCCAAAGAATATCCTCACAGT GCGAAAGATGAAGCACATCCTGTTGAATCAGCCTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_144726 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144726.1, NP_653327.1 |
| RefSeq Size | 3362 bp |
| RefSeq ORF | 2076 bp |
| Locus ID | 153830 |
| UniProt ID | Q96MT1 |
| Domains | RING |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'-linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species. Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function. In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately downregulating cholesterol biosynthesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216033 | RNF145 (Myc-DDK-tagged)-Human ring finger protein 145 (RNF145), transcript variant 2 |
CNY 5800.00 |
|
| RC216033L3 | Lenti-ORF clone of RNF145 (Myc-DDK-tagged)-Human ring finger protein 145 (RNF145), transcript variant 2 |
CNY 7700.00 |
|
| RC216033L4 | Lenti-ORF clone of RNF145 (mGFP-tagged)-Human ring finger protein 145 (RNF145), transcript variant 2 |
CNY 7700.00 |
|
| RG216033 | RNF145 (tGFP-tagged) - Human ring finger protein 145 (RNF145), transcript variant 2 |
CNY 6370.00 |
