OTOA (NM_144672) Human Untagged Clone
CAT＃: SC306178
OTOA (untagged)-Human otoancorin (OTOA), transcript variant 1
CNY 21950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT108; DFNB22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306178 representing NM_144672.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTCAGGAACCTACGACATACTCCCTTTTCCTATTCCTTTTTCTGAGCCATGGAGTGTCGAGTTAT ACAGTGCCAAATTCCAGGCAGGATTTGCATCCATTGTTGCAAAACATGGCGGAAGAAATAATAGATGGA AGCTATCTGAATGCACTGCTGGATCTCATACAGTTTCAAAGCTCCCACGTGTGGACGGATGACCTGTCC CACAGAGTCCTGGCCTATCTGAATTCCCGGAATGTTGCCTTCACCATCCCCAGCCTGCAGGCAGCCGTG GAAAACCACCTGGAGCAGCGTCTGCACCAGCCCCAGAAGCTGCTGGAGGACCTGAGGAAGACAGACGCC CAGCAGTTCCGCACTGCCATGAAATGCCTCTTAGAAGACAAGAAGGACGGCTTGGACCTGAAAGACATC ATCATCGACTTAGGAGAGATTCGAGAACGAGCCTTGCAGAGCCCTGGCGTGAACCGCAGCCTGTTTCTC ATCACACTGGAGAGGTGTTTCCAGATGCTGAACTCCCTGGAGTGTGTGGAGATCCTGGGCAAGGTGCTG AGGGGGTCCTCAGGGAGCTTTCTCCAGCCAGACATCACAGAGCGGCTCCCTCGGGACCTGCGCGAGGAT GCCTTTAAGAACCTATCTGCAGTGTTCAAAGATCTCTACGACAAAACCTCGGCTCATTCCCAGAGAGCT CTCTATTCCTGGATGACTGGAATACTGCAGACATCCTCCAATGCCACTGATGACTCTGCTTCATGGGTC AGTGCGGAACACTTATGGGTTTTGGGCAGATACATGGTTCACCTATCGTTTGAAGAAATTACGAAAATT AGTCCTATAGAAATTGGGCTGTTTATCAGCTATGACAACGCCACCAAGCAGCTGGACATGGTCTATGAC ATCACACCTGAGCTGGCCCAGGCGTTTCTGGAGAGGATCAGCTCCTCCAACTTTAACATGAGGAATACC TCCACCATCCACAGGCTGGGGCTGCTGGTTTGTTTCTACAATGACCTGGAATTGCTGGATGCCACTGTG GCTCAAGTCCTGCTTTACCAGATGATCAAGTGCAGCCACCTGAGGGGCTTCCAGGCTGGCGTCCAGAAG CTCAAAGCAGAACTCCTGGACATTGCCATGGAGAACCAGACCCTCAATGAGACCCTGGGTTCTTTGTCG GATGCAGTTGTAGGTTTGACCTACAGCCAACTGGAATCCCTCTCCCCCGAGGCTGTGCACGGAGCCATC TCCACCCTCAACCAGGTCTCAGGTTGGGCCAAGAGCCAGGTCATCATCTTGTCTGCCAAATACTTGGCC CATGAGAAGGTGCTGTCTTTCTACAATGTCAGCCAGATGGGCGCACTGCTGGCTGGGGTCAGCACCCAG GCCTTCTGCAGCATGAAACGCAAGGACATCTCGCAGGTCCTGAGAAGTGCCGTCTCCCAGTATGTATCC GACTTGTCACCTGCCCAGCAGCAAGGTATCCTCAGCAAGATGGTCCAAGCGGAAGACACTGCCCCAGGC ATCGTGGAGATACAAGGGGCTTTCTTTAAGGAAGTGTCTCTCTTTGATTTAAGGAGGCAACCTGGATTC AACTCTACAGTCCTGAAGGATAAGGAACTTGGAAGGAGCCAGGCTCTGTTCCTGTATGAGCTTCTGTTA AAGACCACCAGAAGGCCTGAGGAGCTTTTGAGTGCTGGGCAGCTGGTCAAAGGCGTGACCTGCTCACAC ATTGATGCCATGAGCACTGACTTCTTTCTGGCCCATTTCCAGGATTTTCAGAACAACTTCGCCCTGCTT TCACCCTATCAGGTTAATTGTTTGGCGTGGAAATACTGGGAAGTTTCCAGATTGTCTATGCCACCTTTC CTCTTGGCTGCACTCCCGGCCCGCTACCTGGCTTCTGTCCCAGCCTCCCAGTGTGTGCCCTTTCTGATC AGCCTGGGGAAGAGCTGGTTGGACTCCTTGGTTTTAGATTCCCACAAAAAGACTTCAGTCCTCAGGAAA GTGCAGCAGTGCCTGGACGACTCCATTGCTGATGAGTACACTGTGGACATCATGGGGAACCTGCTGTGT CACTTGCCGGCAGCCATCATCGACAGGGGGATCTCCCCCAGGGCTTGGGCGACTGCTCTACACGGCCTC AGAGACTGCCCAGACCTCAACCCTGAGCAAAAGGCTGCAGTGAGGCTCAAGCTCCTGGGACAGTATGGA CTCCCTCAGCACTGGACAGCCGAGACCACGAAGGACTTGGGACCCTTTCTAGTACTTTTCTCAGGAGAT GAATTAAGCTCTATAGCCACAAAGTTTCCTGAGATCCTTCTGCAAGCAGCTTCCAAGATGGCCAGGACC CTGCCCACTAAAGAATTCCTCTGGGCTGTCTTTCAGTCTGTTCGGAACAGCAGTGATAAGATCCCCAGC TATGACCCTATGCCTGGTTGCCATGGAGTCGTGGCCCCCTCTTCTGATGACATCTTCAAGTTGGCCGAA GCCAACGCCTGCTGGGCCCTGGAGGACCTGCGGTGCATGGAGGAAGACACATTCATCAGGACCGTGGAA CTGCTGGGAGCTGTCCAGGGTTTCAGCCGGCCTCAGCTGATGACCCTGAAGGAGAAAGCAATACAGGTT TGGGACATGCCATCTTACTGGAGAGAACACCATATCGTCTCCCTGGGGCGCATTGCTCTGGCTCTTAAT GAGAGTGAGCTGGAGCAGCTGGACCTCAGCTCCATAGACACTGTGGCTTCCCTAAGCTGGCAAACAGAA TGGACCCCGGGACAGGCTGAATCCATTTTGCAAGGGTACCTGGATGATTCAGGATACAGTATCCAGGAC CTGAAGAGCTTTCATTTGGTAGGACTTGGTGCAACCCTGTGTGCTATAAACATCACTGAAATCCCACTT ATAAAGATCTCAGAATTCAGGGTGGTAGTGGCCAGAATTGGGACCCTGCTCTGCAGCACACATGTCTTA GCCGAGTTTAAGAGGAAGGCTGAAGTTGTGTTTGGGGATCCCACTGAGTGGACCAGTTCTGTCTTGCAG GAGCTTGGGACCATTGCAGCTGGATTAACTAAGGCAGAGCTCCGGATGCTTGACAAGGATTTGATGCCA TATTTCCAGCCATCAGCAATAAAATGCCTTCCTGATGAGATATTCAAAGAGCTGTCCGCGGAGCAGATC GCCTCCCTGGGTCCGGAGAACGCCGCGGCGGTGACCCACGCCCAGCGCCGGCGGCTCAGTCCACTGCAG CTGCAGAGCCTCCAGCAGGCGCTAGATGGCGCCAAGACTCACTCCTGGCAGGACGCGCCCGCTAGCGCC GGTCCCACTAGAACCTCATCCTCGCGTTCTCCCGCAGGAGCTCTCCAGTCGTGGGGTCTTTGGCTTGGT TGTCCCCTGCTGGTTCTAATGGCCAAGCTCCTGTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_144672 |
| Insert Size | 3420 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144672.3 |
| RefSeq Size | 3624 bp |
| RefSeq ORF | 3420 bp |
| Locus ID | 146183 |
| MW | 126.9 kDa |
| Gene Summary | The protein encoded by this gene is specifically expressed in the inner ear, and is located at the interface between the apical surface of the inner ear sensory epithelia and their overlying acellular gels. It is prposed that this protein is involved in the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in this gene are associated with autosomal recessive deafness type 22 (DFNB22). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221145 | OTOA (Myc-DDK-tagged)-Human otoancorin (OTOA), transcript variant 1 |
CNY 7928.00 |
|
| RC221145L3 | Lenti ORF clone of Human otoancorin (OTOA), transcript variant 1, Myc-DDK-tagged |
CNY 13300.00 |
|
| RC221145L4 | Lenti ORF clone of Human otoancorin (OTOA), transcript variant 1, mGFP tagged |
CNY 13300.00 |
|
| RG221145 | OTOA (tGFP-tagged) - Human otoancorin (OTOA), transcript variant 1 |
CNY 12540.00 |
