CLNK (NM_052964) Human Untagged Clone
CAT＃: SC305708
CLNK (untagged)-Human cytokine-dependent hematopoietic cell linker (CLNK)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MIST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305708 representing NM_052964.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACAGGCAGGGCAATAGAAAGACAACTAAAGAAGGATCCAACGATTTGAAATTCCAGAACTTCAGT CTGCCAAAAAACAGGTCATGGCCTCGCATCAATAGTGCCACAGGCCAGTACCAGAGGATGAACAAGCCT CTTCTAGACTGGGAAAGAAACTTTGCTGCAGTCCTGGATGGAGCAAAAGGCCACAGTGATGATGACTAT GATGACCCTGAGCTTCGGATGGAAGAGACATGGCAGTCGATTAAAATTTTACCAGCCCGGCCTATAAAG GAATCTGAATATGCAGATACACACTATTTCAAGGTTGCAATGGACACTCCCCTTCCGTTAGACACCAGG ACCTCTATCTCCATTGGACAGCCGACCTGGAACACACAGACGAGGTTGGAAAGAGTGGACAAACCCATT TCCAAGGACGTCAGAAGCCAAAACATTAAAGGAGATGCATCCGTAAGAAAGAACAAGATTCCTTTACCA CCTCCTCGGCCTCTCATAACACTTCCGAAGAAGTACCAACCCTTGCCCCCTGAGCCGGAGAGCAGCAGG CCACCTTTATCTCAGAGACACACCTTTCCAGAAGTCCAGAGAATGCCCAGTCAGATAAGCTTAAGGGAC TTAAGTGAGGTCCTTGAAGCAGAAAAAGTTCCTCATAACCAGAGGAAGCCTGAATCAACTCATCTGTTA GAAAACCAAAATACTCAAGAGATTCCACTTGCCATTAGCAGTTCTTCATTCACGACAAGCAACCACAGT GTGCAAAACAGAGATCATAGAGGAGGCATGCAGCCCTGTTCTCCTCAGAGATGCCAGCCTCCAGCCAGC TGCAGCCCTCACGAAAATATACTGCCCTATAAATACACAAGCTGGAGACCACCTTTCCCCAAAAGGTCT GATAGAAAGGATGTCCAGCACAATGAATGGTACATTGGAGAATACAGCCGCCAGGCAGTGGAAGAGGCA TTCATGAAGGAGAACAAGGATGGTAGTTTCTTGGTCCGAGATTGTTCCACAAAATCCAAGGAAGAGCCC TATGTTTTGGCTGTGTTTTATGAGAACAAAGTCTACAATGTAAAAATCCGCTTCCTGGAGAGGAATCAG CAGTTTGCCCTGGGGACAGGACTCAGAGGAGATGAGAAGTTTGATTCAGTAGAAGACATCATCGAACAC TACAAGAATTTTCCCATTATACTAATTGATGGGAAAGATAAAACTGGGGTCCACAGGAAACAGTGTCAC CTCACTCAGCCACTCCCTCTCACCAGACACCTCTTGCCTCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_052964 |
| Insert Size | 1287 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_052964.2 |
| RefSeq Size | 1710 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 116449 |
| UniProt ID | Q7Z7G1 |
| MW | 49.6 kDa |
| Gene Summary | MIST is a member of the SLP76 family of adaptors (see LCP2, MIM 601603; BLNK, MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling, including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A, MIM 147140)-mediated mast cell degranulation (Cao et al., 1999 [PubMed 10562326]; Goitsuka et al., 2000, 2001 [PubMed 10744659] [PubMed 11463797]).[supplied by OMIM, Mar 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213867 | CLNK (Myc-DDK-tagged)-Human cytokine-dependent hematopoietic cell linker (CLNK) |
CNY 3656.00 |
|
| RC213867L3 | Lenti ORF clone of Human cytokine-dependent hematopoietic cell linker (CLNK), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC213867L4 | Lenti ORF clone of Human cytokine-dependent hematopoietic cell linker (CLNK), mGFP tagged |
CNY 5890.00 |
|
| RG213867 | CLNK (tGFP-tagged) - Human cytokine-dependent hematopoietic cell linker (CLNK) |
CNY 4370.00 |
