ARHGEF4 (NM_032995) Human Untagged Clone
CAT＃: SC305530
ARHGEF4 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2
CNY 13020.00
Product images
CNY 1999.00
CNY 3600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASEF; ASEF1; GEF4; STM6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305530 representing NM_032995.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTCCACATGGAG CCTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGACT GTGCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCACCGCTCGCCCGTCAGTGTGGATGACCTG TGGCTGGAGAAGACACAGAGAAAGAAGTTGCAGAAGCAGGCCCACGTCGAAAGGAGGCTGCACATAGGG GCAGTGCACAAAGATGGAGTCAAGTGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTC CCTAGAAGAAGCCATCCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCAC ACACCAGGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGCGAG GAGGACCTGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGGGGTGGGGAGCAG CTGGCTATCAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAAGCACTCTGGGACCATGTCACC ATGGACGACCAGGAGCTGGGCTTCAAAGCTGGGGACGTCATCGAAGTGATGGATGCCACCAACAGAGAG TGGTGGTGGGGCCGGGTCGCCGATGGCGAGGGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAAT CAGGACGAGCCCGCGGATGACGACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAG GCGCAGAGCAGCAAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTAC ATCAAGCACCTGCGCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATGTTCAGC GAGGAGCAGCTGCGTACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAGAAGGCCTTCGTGAAG GCCCTGGAGCAGAGGTTCAACCGCGAGCGCCCACACCTGAGCGAGCTGGGTGCCTGCTTCCTGGAGCAT CAAGCCGACTTCCAGATCTACTCGGAGTACTGCAATAACCACCCCAACGCCTGCGTGGAGCTCTCCCGG CTCACCAAGCTCAGCAAGTACGTGTACTTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATC TCCCTGGATGGCTTCCTGCTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTG CTCAAATACACGCACCCCCAGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAAC GTGGCCCAGCTCATCAACGAGCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAGTGGCAGAGC TCCATAGAGGACTGGGAGGGAGAAGATCTCTTGGTCAGGAGCTCAGAACTCATCTACTCGGGGGAGCTG ACTCGAGTTACACAGCCTCAAGCCAAAAGCCAGCAGCGAATGTTCTTTCTCTTTGACCACCAGCTCATC TACTGTAAGAAGGACCTGCTCCGCCGCGACGTGTTGTACTACAAGGGCCGGCTGGACATGGACGGCCTG GAGGTGGTGGACCTGGAGGACGGGAAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTG CACCGTGGCGCCACAGGGGACAGCCACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTC AAGGCCTTTGCCAGGGAGAGGGAGCAGGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAACTG CAGAGGAAGCAGGCCATGCTGAATGCCAGCAAGCAGCAGGTCACAGGGAAGCCCAAAGGTAGGCGGACA GCAGCCCCACCTCCTCGGCTGCCCGGTCCTTACCCTGCTGACATCATTCCCTTCTCTGAACCACAAAGC CAAGCCAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_032995 |
| Insert Size | 2013 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032995.2 |
| RefSeq Size | 3860 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 50649 |
| UniProt ID | Q9NR80 |
| Protein Families | Druggable Genome |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 76.4 kDa |
| Gene Summary | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (2) has an additional segment in the 3' region, compared to variant 1. This causes the reading frame shift, and the resulting isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216845 | ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 4912.00 |
|
| RC216845L3 | Lenti-ORF clone of ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 9030.00 |
|
| RC216845L4 | Lenti-ORF clone of ARHGEF4 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 9030.00 |
|
| RG216845 | ARHGEF4 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 7890.00 |
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