DDHD1 (NM_030637) Human Untagged Clone
CAT＃: SC305276
DDHD1 (untagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 1
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | iPLA1alpha; PA-PLA1; PAPLA1; SPG28 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_030637 edited
GTTGAATGTGTGGCGGAAGCGCTGGGGGTCACGGCTCCGCGCGCCGCCGGACAGCCGGCG GCGTCTCCACAGCATGAATTACCCGGGCCGCGGGTCCCCACGGAGCCCCGAGCATAACGG CCGAGGCGGCGGCGGCGGCGCCTGGGAGCTGGGCTCAGACGCGAGGCCAGCGTTCGGCGG CGGCGTCTGCTGCTTCGAGCACCTGCCCGGCGGGGACCCGGACGACGGCGACGTGCCCCT GGCCCTGCTGCGCGGGGAACCCGGGCTGCATTTGGCGCCGGGCACCGACGACCACAACCA CCACCTCGCGCTGGACCCCTGCCTCAGTGACGAGAACTATGACTTTAGCTCCGCCGAGTC GGGCTCCTCGCTGCGCTACTACAGCGAGGGTGAGAGCGGCGGCGGCGGCGGCGGCAGCTC CTTGTCGCTGCATCCGCCGCAGCAGCCTCCGCTGGTCCCGACGAACTCGGGGGGCGGCGG CGCGACAGGAGGGTCCCCCGGGGAAAGGAAACGTACCCGGCTTGGCGGCCCGGCGGCCCG GCACCGCTATGAGGTAGTGACGGAGCTGGGCCCGGAGGAGGTACGCTGGTTCTACAAGGA GGACAAGAAGACCTGGAAGCCCTTCATCGGCTACGACTCGCTCCGCATCGAGCTCGCCTT CCGGACCCTGCTGCAGACCACGGGTGCCCGGCCCCAGGGCGGGGACCGGGACGGCGACCA TGTGTGCTCCCCCACGGGCCCAGCCTCCAGTTCCGGAGAAGATGACGATGAGGACCGCGC CTGCGGCTTCTGCCAGAGTACGACGGGGCACGAGCCGGAGATGGTGGAGCTTGTGAACAT CGAGCCTGTGTGCGTGCGGGGCGGCCTCTACGAGGTGGATGTGACCCAAGGAGAGTGCTA CCCGGTGTACTGGAACCAGGCTGATAAAATACCAGTAATGCGTGGACAGTGGTTTATTGA CGGCACTTGGCAGCCTCTAGAAGAGGAAGAAAGTAATTTAATTGAGCAAGAACATCTCAA TTGTTTTAGGGGCCAGCAGATGCAGGAAAATTTCGATATTGAAGTGTCAAAATCCATAGA TGGAAAAGATGCTGTTCATAGTTTCAAGTTGAGTCGAAACCATGTGGACTGGCACAGTGT GGATGAAGTATATCTTTATAGTGATGCAACAACATCTAAAATTGCAAGAACAGTTACCCA AAAACTGGGATTTTCTAAAGCATCAAGTAGTGGTACCAGACTTCATAGAGGTTATGTAGA AGAAGCCACATTAGAAGACAAGCCATCACAGACTACCCATATTGTATTTGTTGTGCATGG CATTGGGCAGAAAATGGACCAAGGAAGAATTATCAAAAATACAGCTATGATGAGAGAAGC TGCAAGAAAAATAGAAGAAAGGCATTTTTCCAACCATGCAACACATGTTGAATTTCTGCC TGTTGAGTGGCGGTCAAAACTTACTCTTGATGGAGACACTGTTGATTCCATTACTCCTGA CAAAGTACGAGGTTTAAGGGATATGCTGAACAGCAGTGCAATGGACATAATGTATTATAC TAGTCCACTTTATAGAGATGAACTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTGTA TTCCCTTTTCTGTTCTCGGAATCCAGACTTTGAAGAAAAAGGGGGTAAAGTCTCAATAGT ATCACATTCCTTGGGATGTGTAATTACTTATGACATAATGACTGGCTGGAATCCAGTTCG GCTGTATGAACAGTTGCTGCAAAAGGAAGAAGAGTTGCCTGATGAACGATGGATGAGCTA TGAAGAACGACATCTTCTTGATGAACTCTATATAACAAAACGACGGCTGAAGGAAATAGA AGAACGGCTTCACGGATTGAAAGCATCATCTATGACACAAACACCTGCCTTAAAATTTAA GGTTGAGAATTTCTTCTGTATGGGATCCCCATTAGCAGTTTTCTTGGCGTTGCGTGGCAT CCGCCCAGGAAATACTGGAAGTCAAGACCATATTTTGCCTAGAGAGATTTGTAACCGGTT ACTAAATATTTTTCATCCTACAGATCCAGTGGCTTATAGATTAGAACCATTAATACTGAA ACACTACAGCAACATTTCACCTGTCCAGATCCACTGGTACAATACTTCAAATCCTTTACC TTATGAACATATGAAGCCAAGCTTTCTCAACCCAGCTAAAGAACCTACCTCAGTTTCAGA GAATGAAGGCATTTCAACCATACCAAGCCCTGTGACCTCACCAGTTTTGTCCCGCCGACA CTATGGAGAATCTATAACAAATATAGGCAAAGCAAGCATATTAGGGGCTGCTAGCATTGG AAAGGGACTTGGAGGAATGTTGTTCTCAAGATTTGGACGTTCATCTACAACACAGTCATC TGAAACATCAAAAGACTCAATGGAAGATGAGAAGAAGCCAGTTGCCTCACCTTCTGCTAC CACCGTAGGGACACAGACCCTTCCACATAGCAGTTCTGGCTTCCTCGATTCTGCATTGGA GTTGGATCACAGGATTGATTTTGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTC AGCTGTCACGTCGCATACTGCCTATTGGTCATCCTTGGATGTTGCCCTTTTTCTTTTAAC CTTCATGTATAAACATGAGCACGATGATGATGCAAAACCCAATTTAGATCCAATCTGAAC TCTTGAAGGACATGAATGGCCTAAAACTGATTTTTTTTTTTTCCGTTAAAATGTGTGTGT CAAGATACGGAGATTTCAGGGTTAAAGTATATTTCAGTTTTCTTTAGGGCAACATATATT TGAATTTAAAAGCACTTTATTTAAAAAAAAAAGAAGTTTTCAGTTCTGAAGAAGTCATTT ACAGTTTGCATCATTTTAATTATGAGTCTGACAAAACCTTCTCCAGAGAATCAAGCAAGA CCTGGATGTGAAGAAGGTTTGGGTAAACTGCATGTAAAGGCTACAAATCACAATCTGATT CCTCCCAAATATAAAGGCATATGGAACATAATGTATTAACCAAAGTATGTTATAAATCAA AAATGGTCAAGGTTCAGCATATTCTATATGAAGATCACAAGGTGGTATCGTTTTAGATTT CTATGAAGGCTTTCATTTGTACATCCCTTTGAAAAAATATAACAGATTTAAAATGTTTTG AATTTAACTTGTTTAGAAAAACTAATGCTTAAAACAATATTTGAACTACTGTATTTATAA TTTATTACCTCTAATTGCTTTATTTCAGTGTATGAGACATTACTGTTTTAATGTTTGCTT TGAACATAATTTAAGAACCAGATTTATTTTCTATAGTGATAAACCCTTTTTTCTCAGAAC TCCATCTTTGTACTCTTCAGATGAATATATAGACACTGTGGCATACATTTTTTTTCATTA AAAACTTATGGCTTCATACAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_030637 unedited
CATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGTTGAATGTGTGGCG GAAGCGCTGGGGGTCACGGCTCCGCGCGCCGCCGGACAGCCGGCGGCGTCTCCACAGCAT GAATTACCCGGGCCGCGGGTCCCCACGGAGCCCCGAGCATAACGGCCGAGGCGGCGGCGG CGGCGCCTGGGAGCTGGGCTCAGACGCGAGGCCAGCGTTCGGCGGCGGCGTCTGCTGCTT CGAGCACCTGCCCGGCGGGGACCCGGACGACGGCGACGTGCCCCTGGCCCTGCTGCGCGG GGAACCCGGGCTGCATTTGGCGCCGGGCACCGACGACCACAACCACCACCTCGCGCTGGA CCCCTGCCTCAGTGACGAGAACTATGACTTTAGCTCCGCCGAGTCGGGCTCCTCGCTGCG CTACTACAGCGAGGGTGAGAGCGGCGGCGGCGGCGGCGGCAGCTCCTTGTCGCTGCATCC GCCGCAGCAGCCTCCGCTGGTCCCGACGAACTCGGGGGGCGGCGGCGCGACAGGAGGGTC CCCCGGGGAAAGGAAACGTACCCGGCTTGGCGGCCCGGCGGCCCGGCACCGCTATGAGGT AGTGACGGAGCTGGGCCCGGAGGAGGTACGCTGGTTCTACAAGGAGGACAAGAAGACCTG GAAGCCCTTCATCGGCTACGACTCGCTCCGCATCGAGCTCGCCTTCCGGACCCTGCTGCA GACCACGGGTGCCCGGCCCCAGGGCGGNGACCGGGACGGCGACCATGTGTGCTCCCCCAC GGGCCCAGCCTNCAGTCCGGAGAAGAGACGATGAGGACCGCGCCTNGGCGCTCTGCCAGA GTACGAA >OriGene 3' read for NM_030637 unedited
CCCCGAGTCAATGAATATGAAAAAAAGTATGCCCAGGGAAAATATTTTCTCTGAGAGTAC CAAGAGGGAGTTCTGAGAAAAAAGGGTTTATCACTATAGAAAATAAATCTGGTTCTTAAA TTATGTTCAAAGCAAACATTAAAACAGTAATGTCTCATACTCTGAAATAAAGCAATTAGA GGTAATAAATTATAAATACAGTAGTTCAAATATTGTTTTAAGCATTAGTTTTTCTAAACA AGTTAAATTCAAAACATTTTAAATCTGTTATATTTTTTCAAAGGGATGTACAAATGAAAG CCTTCATAGAAATCTAAAACGATACCACCTTGTGATCTTCATATAGAATATGCTGAACCT TGACCATTTTTGATTTATAACATACTTTGGTTAATACATTATGTTCCATATGCCTTTATA TTTGGGAGGAATCAAATTGTGATTTGTAGCCTTTACATGCAGTTTACCCAAACCTTCTTA ACATCCAGGTCTTGCTTGATACTCTGGAGAAGGTTTTGTCCAACTCATAATTAAAATGAT GCCAACTGTAAATGACTTCTTCATAACTGAAAACTTCTTTTTTTTTTTAAATAAAATGCT TTTAAATTCACATATATGTTGCCCTCAACAAAACTGAAATATACTTTAAGACCTGAAATC TTCGTACTTGAAACACACATTTTAACCGGAAAAAAAAAAAATAACTTCTAGGCCTTTCCT GTCCCTCAATAGACGAGAATGTATATAAATTGGGTTTTTCAATAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_030637 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by two amino acids. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030637.1, NP_085140.1 |
| RefSeq Size | 3563 bp |
| RefSeq ORF | 2619 bp |
| Locus ID | 80821 |
| UniProt ID | Q8NEL9 |
| Gene Summary | This gene is a member of the intracellular phospholipase A1 gene family. The protein encoded by this gene preferentially hydrolyzes phosphatidic acid. It is a cytosolic protein with some mitochondrial localization, and is thought to be involved in the regulation of mitochondrial dynamics. Overexpression of this gene causes fragmentation of the tubular structures in mitochondria, while depletion of the gene results in mitochondrial tubule elongation. Deletion of this gene in male mice caused fertility defects, resulting from disruption in the organization of the mitochondria during spermiogenesis. In humans, mutations in this gene have been associated with hereditary spastic paraplegia (HSP), also known as Strumpell-Lorrain disease, or, familial spastic paraparesis (FSP). This inherited disorder is characterized by progressive weakness and spasticity of the legs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205548 | DDHD1 (Myc-DDK-tagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 1 |
CNY 6384.00 |
|
| RC205548L3 | Lenti ORF clone of Human DDHD domain containing 1 (DDHD1), transcript variant 1, Myc-DDK-tagged |
CNY 9030.00 |
|
| RC205548L4 | Lenti ORF clone of Human DDHD domain containing 1 (DDHD1), transcript variant 1, mGFP tagged |
CNY 9030.00 |
|
| RG205548 | DDHD1 (tGFP-tagged) - Human DDHD domain containing 1 (DDHD1), transcript variant 1 |
CNY 7984.00 |
