Sperm Flagellar 2 (SPEF2) (NM_024867) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT122; KPL2; SPGF43
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC305171 representing NM_024867. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGAGATCCTGTGCCAGTGGCTCAACAAGGAGTTGAAGGTGTCCCGGACCGTGAGTCCCAAGTCA TTTGCAAAGGCATTTTCCAGTGGCTATCTACTTGGAGAAGTTCTACACAAGTTTGAACTTCAGGATGAT TTTTCAGAATTTTTGGACAGCAGGGTTTCAAGTGCCAAACTTAATAATTTTTCTCGCTTGGAGCCAACA CTTAACCTTCTGGGTGTGCAGTTTGATCAGAATGTGGCCCATGGCATCATCACAGAAAAGCCTGGGGTG GCAACAAAGCTGTTATATCAATTGTACATTGCTCTTCAGAAAAAGAAGAAAAGTGGACTGACTGGAGTG GAGATGCAAACCATGCAACGTCTGACAAATTTAAGACTTCAAAACATGAAAAGTGATACTTTTCAAGAG AGACTTAGACACATGATACCACGTCAAACTGATTTCAATCTGATGCGGATTACATACAGGTTTCAAGAA AAATATAAACACGTGAAAGAAGATCTTGCCCATTTGCATTTTGAGAAACTTGAAAGATTTCAAAAACTC AAGGAAGAGCAAAGATGTTTTGATATTGAAAAGCAATACTTAAACAGAAGACGACAAAATGAAATAATG GCCAAAATCCAAGCAGCTATTATACAGATTCCTAAACCTGCATCAAATCGTACTTTGAAAGCACTCGAG GCCCAAAAAATGATGAAAAAGAAAAAAGAGGCAGAAGATGTGGCTGATGAAATTAAGAAGTTCGAAGCA TTAATAAAAAAGGATCTCCAAGCAAAAGAAAGTGCATCCAAGACTTCTTTAGATACAGCAGGCCAGACA ACCACCGATTTGTTAAATACTTACTCAGATGACGAGTACATTAAAAAAATCCAGAAGCGCCTTGAAGAA GATGCTTTTGCACGAGAGCAAAGGGAGAAAAGACGGCGGAAATTGTTAATGGACCAGTTAATAGCCCAC GAAGCACAAGAGGAGGCTTATCGGGAGGAACAGCTGATTAACCGGCTGATGCGGCAGTCCCAGCAGGAG CGCAGGATTGCCGTGCAGCTCATGCATGTTCGGCATGAAAAGGAAGTTTTATGGCAAAACAGAATTTTC AGAGAAAAACAACATGAGGAAAGACGACTTAAAGATTTCCAGGATGCTCTTGATCGAGAAGCGGCTTTG GCAAAACAAGCCAAGATTGACTTTGAAGAACAATTCCTTAAAGAAAAGAGATTTCATGATCAGATTGCT GTGGAAAGAGCTCAAGCTCGTTATGAAAAGCATTATTCAGTATGTGCAGAAATTTTGGATCAAATAGTT GATTTGTCCACTAAAGTGGCAGACTATCGAATGTTGACAAATAATCTGATTCCGTATAAGTTGATGCAT GATTGGAAGGAACTATTTTTTAATGCAAAACCCATATATGAACAAGCCTCTGTTAAGACACTACCTGCT AACCCCTCAAGAGAACAACTTACAGAACTGGAGAAAAGGGACTTGCTAGATACCAATGATTATGAAGAA TATAAGAACATGGTTGGAGAGTGGGCCTTACCAGAAGAAATGGTTGACAATTTACCACCCTCCAACAAT TGCATACTGGGCCATATTCTTCACAGGCTAGCTGAAAAATCTCTTCCTCCTCGAGCGGAATCAACAACA CCTGAATTACCTTCATTTGCTGTTAAAGGATGCTTATTGGGGAAAACATTAAGTGGAAAAACTACCATT TTAAGGTCTCTACAAAAAGACTTTCCTATACAGATACTTTCTATTGACACTCTTGTCCAAGAAGCTATC CAAGCATTTCATGACAATGAAAAAGTCAGTGAGGTTCTACCAATTCAGAAAAATGATGAAGAAGATGCT CTACCAGTTCTGCAAGAGGAGATTAAAGAAAGCCAGGATCCACAACATGTATTTTCAGCTGGTCCAGTT TCAGATGAAGTATTACCAGAAACAGAAGGTGAAACAATGCTTAGTGCTAATGCTGATAAAACACCAAAA GCTGAAGAAGTCAAATCAAGTGATAGTTTCTTAAAACTCACTACACGTGCTCAGCTTGGTGCAAAATCA GAACAGTTGCTGAAGAAAGGAAAGAGCATTCCTGATGTGCTGCTTGTTGACATCATAGTAAATGCTATT AATGAGATACCTGTGAATCAAGACTGTATCCTAGATGGTTTTCCAATGACTTTAAACCAAGCACAGCTT CTGGAAGAAGCTCTTACAGGCTGCAATAGAAACCTCACAGAAGTGGAAAGAAAAAAAGCACAAAAATCC ACATTGGCTATTGATCCTGCGACTTCCAAAGAAATACCTCTTCCCTCTCCTGCATTTGATTTTGTCATA TTATTAGATGTTTCAGATACTTCCTCAATGAGTCGCATGAATGATATTATAGCTGAAGAATTGTCCTAT AAAACTGCTCACGAAGATATCAGTCAACGTGTAGCTGCTGAAAACCAAGATAAGGATGGAGACCAAAAT TTAAGAGACCAGATACAACATAGAATTATAGGCTTCTTGGACAACTGGCCTTTATTGGAGCAATGGTTT TCAGAGCCAGAAAATATTTTGATAAAAATCAATGCTGAAATAGATAAGGAATCTTTATGTGAAAAAGTA AAAGAAATTCTTACGACTGAAATAGCAAAAAAAAAGAATAAAGTTGAGAAGAAATTAGAAGAAAAGGAA GCTGAGAAAAAAGCAGCAGCTTCCCTGGCTGAGCTTCCACTTCCTACACCTCCTCCTGCTCCTCCTCCT GAACCAGAAAAAGAGAAGGAAATTCATCAAAGCCATGTGGCTTCAAAAACTCCTACTGCAAAAGGAAAA CCTCAATCAGAAGCCCCGCATGGTAAGCAAGAATCTCTTCAGGAAGGAAAAGGGAAGAAAGGTGAGACC GCACTCAAAAGAAAAGGTTCTCCTAAAGGAAAATCATCAGGAGGAAAAGTACCAGTAAAGAAATCACCT GCTGACTCTACAGATACATCACCTGTTGCAATAGTGCCACAGCCACCTAAGCCAGGATCAGAAGAATGG GTCTATGTGAATGAACCAGTTCCTGAGGAAATGCCTTTGTTTTTAGTACCTTACTGGGAACTAATAGAA AATTCCTATATAAACACCATCAAAACAGTACTCAGGCATCTGAGGGAAGACCAGCATACTGTGCTTGCT TACTTATATGAGATTAGAACAAGTTTCCAGGAGTTTCTAAAGCGTCCGGATCACAAGCAAGATTTTGTA GCTCAATGGCAGGCTGATTTCAACTCCCTTCCTGATGACCTGTGGGATGATGAGGAAACAAAGGCTGAA CTACATCAACGAGTGAATGATCTGCGAGACCGCCTGTGGGACATTTGTGATGCCCGGAAGGAAGAGGCG GAGCAGGAGCGGCTTGACATCATTAATGAGAGCTGGTTACAGGACACTCTTGGAATGACAATGAACCAT TTCTTTTCCCTGATGCAGGCAGAGCTGAACCGTTTCCAAGATACAAAGAGACTCCTTCAAGATTATTAC TGGGGAATGGAAAGTAAAATCCCAGTAGAGGACAACAAGAGATTTACTCGAATCCCTTTGGTCCAACTG GATAGTAAAGACAATTCTGAAAGCCAGCTTAGAATTCCTCTAGTTCCTCGAATATCCATTTCTCTGGAA ACAGTTACACCCAAACCAAAAACAAAATCAGTACTGAAGGGCAAGATGGATAACTCCCTAGAAAACGTT GAGTCCAACTTTGAGGCCGATGAAAAGTTGGTCATGGACACCTGGCAGCAGGCTTCTTTAGCAGTATCT CACATGGTGGCTGCTGAAATTCATCAGAGGCTTATGGAAGAAGAAAAAGAAAACCAGCCAGCAGACCCC AAAGAAAAATCTCCTCAGATGGGTGCAAATAAAAAAGTCAAAAAGGAGCCACCCAAGAAAAAACAGGAA GACAAAAAACCCAAAGGTAAATCACCACCTATGGCAGAAGCAACTCCTGTCATAGTAACAACAGAGGAA ATTGCTGAAATCAAAAGGAAAAATGAACTGAGGGTCAAAATAAAAGAAGAACACCTTGCTGCCTTGCAA TTTGAAGAAATAGCCACGCAATTTCGACTTGAACTGATAAAGACAAAAGCATTGGCTCTTCTTGAAGAT TTAGTAACAAAGGTGGTTGATGTATATAAACTCATGGAAAAATGGCTTGGTGAGAGGTATTTGAATGAA ATGGCCAGTACAGAAAAATTAACTGACGTAGCTCGCTATCACATTGAAACATCTACAAAAATTCAGAAT GAACTTTATTTAAGCCAAGAAGACTTCTTCATTAATGGCAATATAAAAGTCTTCCCAGATCCTCCCCCA TCAATACGTCCTCCACCTGTAGAAAAGGAAGAAGATGGTACCCTGACCATTGAACAGCTTGACAGTCTT CGAGATCAGTTCTTAGATATGGCACCTAAAGGCATAATAGGAAATAAAGCATTTACTGACATTCTGATC GATTTGGTGACCCTGAACCTTGGCACAAACAACTTTCCTAGTAATTGGATGCACCTTACCCAACCTGAA TTACAGGAATTAACATCTTTATTAACAGTCAACTCCGAGTTCGTGGACTGGCGGAAGTTCCTGTTAGTA ACCTCAATGCCTTGGCCCATTCCCTTGGAGGAGGAGCTCCTTGAGACCCTTCAGAAGTTCAAGGCTGTG GATAAGGAGCAGTTGGGCACCATCACTTTTGAGCAGTATATGCAGGCTGGTCTGTGGTTTACAGGAGAT GAAGATATAAAAATTCCAGAAAATCCTCTTGAACCCCTTCCATTTAATAGGCAGGAGCATCTTATAGAG TTTTTCTTTAGGCTATTTGCTGACTATGAGAAGGATCCACCCCAGCTTGACTACACACAGATGCTGCTT TACTTTGCTTGCCACCCAGACACCGTGGAAGGAGTCTACAGGGCCCTCAGTGTGGCTGTTGGAACTCAT GTCTTCCAACAAGTCAAAGCTTCCATTCCAAGTGCAGAAAAGACCTCCTCAACTGATGCAGGTCCAGCT GAGGAATTTCCTGAACCTGAGGAAAATGCTGCAAGAGAAGAAAGGAAATTAAAAGACGACACGGAGAAA AGGGAACAGAAGGATGAAGAAATCCCTGAAAATGCAAACAATGAAAAGATGTCCATGGAAACACTACTC AAAGTGTTCAAAGGGGGAAGTGAAGCACAGGACTCCAATAGATTTGCCAGCCACCTAAAGATAGAGAAC ATTTATGCAGAGGGCTTCATAAAAACATTTCAAGACCTAGGTGCCAAGAACCTGGAGCCAATTGAAGTC GCTGTTCTCTTGAAGCATCCTTTTATTCAAGACCTGATTTCAAATTATTCAGACTATAAGTTTCCTGAT ATTAAAATAATTCTCCAAAGGAGTGAACATGTACAAGGAAGTGATGGAGAGAGATCACCTTCAAGACAT ACAGAGGAAAAGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_024867
Insert Size	5469 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024867.3
RefSeq Size	5651 bp
RefSeq ORF	5469 bp
Locus ID	79925
UniProt ID	Q9C093
MW	209.8 kDa
Gene Summary	Required for correct axoneme development in spermatozoa. Important for normal development of the manchette and sperm head morphology. Essential for male fertility. Plays a role in localization of the intraflagellar transport protein IFT20 to the manchette, suggesting function as an adapter for dynein-mediated protein transport during spermatogenesis. Also plays a role in bone growth where it seems to be required for normal osteoblast differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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